专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09435799B2 Methods and reagents for improved selection of biological materials 有权
标题翻译：改善生物材料选择的方法和试剂
公开(公告)号：US09435799B2
公开(公告)日：2016-09-06
申请号：US10208939
申请日：2002-07-31
申请人： Thomas R Russell , Pauline McGann , Mike Musick , Michael Ciocci
发明人： Thomas R Russell , Pauline McGann , Mike Musick , Michael Ciocci
IPC分类号： G01N33/543
CPC分类号： G01N33/5434 , G01N33/54326 , G01N33/54346 , G01N33/54353 , G01N33/553 , G01N33/577 , G01N2440/32
摘要： Methods, apparatus and compositions for separating a desired or undesired population or subpopulation from a biological sample are disclosed herein. The selection procedure is based on ferromagnetic, dense particles in a preferred size range from about 0.8 to about 1.2 microns. Specific binding agents are bound to the particles that recognize and bind to specific molecules on the targeted population or subpopulation, and the particles are mixed with the sample in such a way as to promote movement of the particles relative to the sample, promoting binding to the targeted population or subpopulation without non-specifically binding to non-targeted populations in the sample. Because of the large particle density, the bound population is separated from the fluid sample by gravity. Alternatively, the sample, including the bound, targeted population, is placed in a magnetic field such that the particles separate from the sample by evenly distributing over the vessel wall thus limiting non-specific trapping of the non-targeted population.
摘要翻译：本文公开了用于从生物样品中分离所需或不需要的群体或亚群体的方法，装置和组合物。选择程序基于约0.8至约1.2微米的优选尺寸范围内的铁磁致密颗粒。特异性结合剂与识别并结合目标群体或亚群体上的特定分子的颗粒结合，并且颗粒与样品混合以促进颗粒相对于样品的移动，促进与目标群体或亚群，而不特异性地结合样本中的非目标群体。由于粒子密度较大，结合的群体通过重力与流体样本分离。或者，将包括结合的目标群体的样品置于磁场中，使得颗粒通过均匀分布在血管壁上而与样品分离，从而限制非目标群体的非特异性捕获。

IPRDB

热门服务

关于我们

友情链接

联系方式