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1. 发明申请

US20120270309A1 THERMAL CYCLER 审中-公开
标题翻译：热循环
公开(公告)号：US20120270309A1
公开(公告)日：2012-10-25
申请号：US13389479
申请日：2010-08-18
申请人： Tateki Arai , Osamu Takeda , Hiroyuki Izu , Hiroyuki Mukai
发明人： Tateki Arai , Osamu Takeda , Hiroyuki Izu , Hiroyuki Mukai
IPC分类号： C12M1/00
CPC分类号： B01L7/52 , B01L3/50825 , B01L2200/147 , B01L2300/1827
摘要： A thermal cycler for incubating reaction mixture, the thermal cycler including:(1) a heat block for holding and heating reaction containers which contain the reaction mixture, the heat block being a double-layered structure of a lower layer heat block and an upper layer heat block; and (2) a thermal control means for controlling a temperature of the lower layer heat block and a temperature of the upper layer heat block independently and respectively and keeping the temperature of the upper layer heat block higher than the temperature of the lower layer heat block while incubating the reaction mixture. Since the thermal cycler prevents the occurrence of the condensation of water or other components of the reaction mixture in the reaction container and the thermal differences based on the installation positions of the reaction containers, the PCR method and the other enzyme reaction can be performed in a good repeatability.
摘要翻译：一种用于温育反应混合物的热循环仪，所述热循环仪包括：（1）用于保持和加热含有反应混合物的反应容器的热块，所述加热块是下层加热块和上层的双层结构热块; 和（2）分别独立地分别控制下层热块的温度和上层热块的温度的热控制装置，并且保持上层热块的温度高于下层热块的温度同时孵育反应混合物。由于热循环仪防止反应容器中反应混合物的水或其它组分的冷凝发生，以及基于反应容器的安装位置的热差异，PCR法和其它酶反应可以在良好的重复性。

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