专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08883692B2 Method for cell surface displaying of target proteins using Bacillus anthracis exosporium 有权
标题翻译：使用炭疽杆菌外芽的细胞表面显示靶蛋白的方法
公开(公告)号：US08883692B2
公开(公告)日：2014-11-11
申请号：US12159933
申请日：2006-12-29
申请人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
发明人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
IPC分类号： C40B40/10 , C12P21/02 , C07K16/00 , C07K14/32 , C12N15/70
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BclA或其片段的bclA基因的表达载体，并且靶蛋白可以以与BclA或BclA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BclA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

2. 发明申请

US20130015559A1 SEMICONDUCTOR DEVICES AND METHODS OF MANUFACTURING THE SAME 有权
标题翻译：半导体器件及其制造方法
公开(公告)号：US20130015559A1
公开(公告)日：2013-01-17
申请号：US13546415
申请日：2012-07-11
申请人： Sung-Ho LEE , Jin Choi , Yong-Ho Yoo , Jong-Hyuk Kang , Hyun-Joo Cha , Hee-Dong Park , Tae-Jung Park
发明人： Sung-Ho LEE , Jin Choi , Yong-Ho Yoo , Jong-Hyuk Kang , Hyun-Joo Cha , Hee-Dong Park , Tae-Jung Park
IPC分类号： H01L27/08 , H01L21/82
CPC分类号： H01L28/91 , H01L27/10817 , H01L27/10852 , H01L28/60
摘要： A semiconductor device includes a plurality of lower electrodes on a substrate, with each of the lower electrodes extending in a height direction from the substrate and including sidewalls, the lower electrodes being spaced apart from each other in a first direction and in a second direction, a plurality of first supporting layer patterns contacting the sidewalls of the lower electrodes, the first supporting layer patterns extending in the first direction between ones of the lower electrodes adjacent in the second direction, a plurality of second supporting layer patterns contacting the sidewalls of the lower electrodes, the second supporting layer pattern extending in the second direction between ones of the lower electrodes adjacent in the first direction, the plurality of second supporting layer patterns being spaced apart from the plurality of first supporting layer patterns in the height direction.
摘要翻译：半导体器件包括在基片上的多个下电极，其中每个下电极从衬底沿高度方向延伸并且包括侧壁，下电极在第一方向和第二方向彼此间隔开，与下电极的侧壁接触的多个第一支撑层图案，第一支撑层图案沿着第一方向在第二方向上相邻的下电极之间延伸;多个第二支撑层图案，其与下部电极的侧壁接触; 电极，所述第二支撑层图案沿着所述第二方向在与所述第一方向相邻的所述下电极中的所述第二方向延伸，所述多个第二支撑层图案在所述高度方向上与所述多个第一支撑层图案间隔开。

3. 发明授权

US08519045B2 Graphene composite nanofiber and preparation method thereof 有权
标题翻译：石墨烯复合纳米纤维及其制备方法
公开(公告)号：US08519045B2
公开(公告)日：2013-08-27
申请号：US12712653
申请日：2010-02-25
申请人： Sung-Yeon Jang , Ho Seok Park , Seong Mu Jo , Dong Young Kim , Won Hi Hong , Sang Yup Lee , Tae Jung Park , Bong Gill Choi
发明人： Sung-Yeon Jang , Ho Seok Park , Seong Mu Jo , Dong Young Kim , Won Hi Hong , Sang Yup Lee , Tae Jung Park , Bong Gill Choi
IPC分类号： C08K3/04
CPC分类号： D01F9/14 , B82Y30/00 , B82Y40/00 , C01B32/194 , C01B32/23 , D01D5/0069 , D01D5/0076 , D01D5/14 , D01F1/10
摘要： Disclosed are a graphene composite nanofiber and a preparation method thereof. The graphene composite nanofiber is produced by dispersing graphenes to at least one of a surface and inside of a polymer nanofiber or a carbon nanofiber having a diameter of 1˜1000 nm, and the graphenes include at least one type of monolayer graphenes, and multilayer graphenes having a thickness of 10 nm or less. The graphene composite nanofiber can be applied to various industrial fields, e.g., a light emitting display, a micro resonator, a transistor, a sensor, a transparent electrode, a fuel cell, a solar cell, a secondary cell, and a composite material, owing to a unique structure and property of graphene.
摘要翻译：公开了一种石墨烯复合纳米纤维及其制备方法。石墨烯复合纳米纤维通过将石墨烯分散在聚合物纳米纤维或直径为1〜1000nm的碳纳米纤维的表面和内部中的至少一个上，并且所述石墨烯包括至少一种类型的单层石墨烯和多层石墨烯厚度为10nm以下。石墨烯复合纳米纤维可以应用于各种工业领域，例如发光显示器，微谐振器，晶体管，传感器，透明电极，燃料电池，太阳能电池，二次电池和复合材料，由于石墨烯的独特结构和性质。

4. 发明授权

US08389225B2 Bio-silica chip comprising silica binding protein and method for fabricating the same 有权
标题翻译：包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法
公开(公告)号：US08389225B2
公开(公告)日：2013-03-05
申请号：US12410071
申请日：2009-03-24
申请人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
发明人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N33/552 , G01N2035/00158
摘要： The present invention relates to a bio-silica chip comprising a silica-binding protein and a fabrication method thereof, and more particularly to a bio-silica chip in which a fusion protein of a silica-binding protein and a probe protein is immobilized on a chip comprising a silica layer, a fabrication method thereof and a method of using the bio-silica chip to detect interactions with biomaterials. The bio-silica chip will be very useful in biosensors, etc., because the bio-silica chip is advantageous in that it does not cause non-specific protein binding in the detection of protein-DNA, protein-ligand, protein-antibody, protein-peptide, protein-carbohydrate, protein-protein and cell-biomaterial interactions. Also, in the method for fabricating the bio-silica chip, a probe chip can be selectively immobilized on a silica device chip, which is widely used in biosensors, without a chemical surface treatment process. Thus, a chip fabricating process is simplified and a complicated process for purifying the probe protein becomes unnecessary, thus providing great improvements in productivity and economic efficiency.
摘要翻译：本发明涉及包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法，更具体地说，涉及将二氧化硅结合蛋白质和探针蛋白质的融合蛋白质固定在其上的生物二氧化硅芯片包括二氧化硅层的芯片，其制造方法以及使用生物二氧化硅芯片来检测与生物材料的相互作用的方法。生物二氧化硅芯片在生物传感器等方面非常有用，因为生物二氧化硅芯片是有利的，因为它不会在检测蛋白质-DNA，蛋白质 - 配体，蛋白质 - 抗体等过程中引起非特异性蛋白质结合，蛋白质 - 肽，蛋白质 - 碳水化合物，蛋白质 - 蛋白质和细胞 - 生物材料相互作用。此外，在制造生物二氧化硅芯片的方法中，探针芯片可以选择性地固定在广泛用于生物传感器的二氧化硅器件芯片上，而无需化学表面处理工艺。因此，简化了芯片制造工艺，并且不需要用于纯化探针蛋白的复杂过程，从而提高了生产率和经济效率。

5. 发明申请

US20120049257A1 SEMICONDUCTOR DEVICE 审中-公开
标题翻译：半导体器件
公开(公告)号：US20120049257A1
公开(公告)日：2012-03-01
申请号：US13208997
申请日：2011-08-12
申请人： SungHo Lee , Jin Choi , Yong-Ho Yoo , Jong-Hyuk Kang , Hyun-Joo Cha , Tae-Jung Park
发明人： SungHo Lee , Jin Choi , Yong-Ho Yoo , Jong-Hyuk Kang , Hyun-Joo Cha , Tae-Jung Park
IPC分类号： H01L27/108 , H01L29/92
CPC分类号： H01L27/10814 , H01L27/10852 , H01L28/90
摘要： A DRAM device can include a plurality of capacitors that are arranged in a line in a first direction. Each of the capacitors can include an upper electrode. A contact pattern having a line shape can extend in the first direction and can be electrically connected to each of the upper electrodes. A conductor can be on the contact pattern opposite the upper electrodes and can be electrically connected to the contact pattern.
摘要翻译： DRAM装置可以包括沿第一方向排列成一行的多个电容器。每个电容器可以包括上电极。具有线状的接触图案可以在第一方向上延伸并且可以电连接到每个上电极。导体可以在与上电极相对的接触图案上，并且可以电连接到接触图案。

6. 发明申请

US20110124131A1 METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN 有权
标题翻译：使用金属结合蛋白制备金属纳米颗粒的方法
公开(公告)号：US20110124131A1
公开(公告)日：2011-05-26
申请号：US12301581
申请日：2007-04-17
申请人： Sang Yup Lee , Tae Jung Park
发明人： Sang Yup Lee , Tae Jung Park
IPC分类号： G01N33/553 , C12P3/00 , B32B5/16 , C12N1/21 , C12N1/16 , C12N1/00 , B05D7/14 , C12N1/15
CPC分类号： C07K14/825 , B82Y5/00 , B82Y15/00 , C12N9/104 , C12P3/00 , G01N33/531 , G01N33/588 , Y10T428/2982
摘要： The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, en the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.
摘要翻译：本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。更具体地，涉及一种制备重金属结构体的方法，包括以下步骤：在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物，以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物，以及根据所述方法制备的重金属结构纳米颗粒。与通过物理结合金属材料制备量子点的现有方法不同，本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。此外，量子点是有用的，因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。

7. 发明申请

US20100317790A1 GRAPHENE COMPOSITE NANOFIBER AND PREPARATION METHOD THEREOF 有权
标题翻译：石墨复合纳米纤维及其制备方法
公开(公告)号：US20100317790A1
公开(公告)日：2010-12-16
申请号：US12712653
申请日：2010-02-25
申请人： Sung-Yeon JANG , Ho Seok PARK , Seong Mu JO , Dong Young KIM , Won Hi HONG , Sang Yup LEE , Tae Jung PARK , Bong Gill CHOI
发明人： Sung-Yeon JANG , Ho Seok PARK , Seong Mu JO , Dong Young KIM , Won Hi HONG , Sang Yup LEE , Tae Jung PARK , Bong Gill CHOI
IPC分类号： C08K3/04 , B29C47/00
CPC分类号： D01F9/14 , B82Y30/00 , B82Y40/00 , C01B32/194 , C01B32/23 , D01D5/0069 , D01D5/0076 , D01D5/14 , D01F1/10
摘要： Disclosed are a graphene composite nanofiber and a preparation method thereof. The graphene composite nanofiber is produced by dispersing graphenes to at least one of a surface and inside of a polymer nanofiber or a carbon nanofiber having a diameter of 1˜1000 nm, and the graphenes include at least one type of monolayer graphenes, and multilayer graphenes having a thickness of 10 nm or less. The graphene composite nanofiber can be applied to various industrial fields, e.g., a light emitting display, a micro resonator, a transistor, a sensor, a transparent electrode, a fuel cell, a solar cell, a secondary cell, and a composite material, owing to a unique structure and property of graphene.
摘要翻译：公开了一种石墨烯复合纳米纤维及其制备方法。石墨烯复合纳米纤维通过将石墨烯分散在聚合物纳米纤维或直径为1〜1000nm的碳纳米纤维的表面和内部的至少一个中，并且所述石墨烯包括至少一种类型的单层石墨烯和多层石墨烯厚度为10nm以下。石墨烯复合纳米纤维可以应用于各种工业领域，例如发光显示器，微谐振器，晶体管，传感器，透明电极，燃料电池，太阳能电池，二次电池和复合材料，由于石墨烯的独特结构和性质。

8. 发明申请

US20100035362A1 BIO-SILICA CHIP COMPRISING SILICA BINDING PROTEIN AND METHOD FOR FABRICATING THE SAME 有权
标题翻译：包含二氧化硅结合蛋白的二氧化硅芯片及其制造方法
公开(公告)号：US20100035362A1
公开(公告)日：2010-02-11
申请号：US12410071
申请日：2009-03-24
申请人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
发明人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
IPC分类号： G01N33/53 , G01N33/00 , C07K14/00 , C07H21/00 , C12N15/63 , C12N1/21 , C12N1/15 , C12N1/19 , C12P21/00
CPC分类号： G01N33/552 , G01N2035/00158
摘要： The present invention relates to a bio-silica chip comprising a silica-binding protein and a fabrication method thereof, and more particularly to a bio-silica chip in which a fusion protein of a silica-binding protein and a probe protein is immobilized on a chip comprising a silica layer, a fabrication method thereof and a method of using the bio-silica chip to detect interactions with biomaterials. The bio-silica chip will be very useful in biosensors, etc., because the bio-silica chip is advantageous in that it does not cause non-specific protein binding in the detection of protein-DNA, protein-ligand, protein-antibody, protein-peptide, protein-carbohydrate, protein-protein and cell-biomaterial interactions. Also, in the method for fabricating the bio-silica chip, a probe chip can be selectively immobilized on a silica device chip, which is widely used in biosensors, without a chemical surface treatment process. Thus, a chip fabricating process is simplified and a complicated process for purifying the probe protein becomes unnecessary, thus providing great improvements in productivity and economic efficiency
摘要翻译：本发明涉及包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法，更具体地说，涉及将二氧化硅结合蛋白质和探针蛋白质的融合蛋白质固定在其上的生物二氧化硅芯片包括二氧化硅层的芯片，其制造方法以及使用生物二氧化硅芯片来检测与生物材料的相互作用的方法。生物二氧化硅芯片在生物传感器等方面非常有用，因为生物二氧化硅芯片是有利的，因为它不会在检测蛋白质-DNA，蛋白质 - 配体，蛋白质 - 抗体等过程中引起非特异性蛋白质结合，蛋白质 - 肽，蛋白质 - 碳水化合物，蛋白质 - 蛋白质和细胞 - 生物材料相互作用。此外，在制造生物二氧化硅芯片的方法中，探针芯片可以选择性地固定在广泛用于生物传感器的二氧化硅器件芯片上，而无需化学表面处理工艺。因此，简化了芯片制造工艺，并且不需要用于纯化探针蛋白的复杂过程，从而提高了生产率和经济效率

9. 发明申请

US20050176096A1 Stabilized biocatalysts and methods of bioconversion using the same 审中-公开
标题翻译：稳定的生物催化剂和使用其的生物转化方法
公开(公告)号：US20050176096A1
公开(公告)日：2005-08-11
申请号：US10510744
申请日：2002-04-09
申请人： Seok-Joon Kwon , Soo Keun Choi , Heung Chae Jung , Jae Gu Pan , Joon Sung Chang , Tae Jung Park
发明人： Seok-Joon Kwon , Soo Keun Choi , Heung Chae Jung , Jae Gu Pan , Joon Sung Chang , Tae Jung Park
IPC分类号： C12N3/00 , C12N11/16 , C12N15/74 , C12N15/75 , C12P19/44 , C12P21/06 , C12N1/21
CPC分类号： C12P19/44 , C12N3/00 , C12N11/16 , C12N15/74 , C12N15/75
摘要： The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusion form and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.
摘要翻译：本发明涉及在孢子或病毒表面上显示的生物催化剂和使用其的生物转化方法，特别涉及使用生物催化剂的生物转化方法，其包括以下步骤：（a）制备用于孢子表面展示的载体，包括包含编码显示基序的基因和编码生物催化剂的基因的基因构建体，其中当表达时，所述基因构建体以融合形式表达显示基序和生物催化剂，并且生物催化剂显示在孢子表面上; （b）用载体转染宿主细胞用于孢子表面显示; （c）在宿主细胞的孢子表面上显示生物催化剂; （d）回收其表面上显示生物催化剂的孢子; 和（e）使用在其表面上显示生物催化剂的孢子和生物催化剂进行生物转化反应。

10. 发明授权

US08476055B2 Method for preparing metal nanoparticle using metal binding protein 有权
标题翻译：使用金属结合蛋白制备金属纳米粒子的方法
公开(公告)号：US08476055B2
公开(公告)日：2013-07-02
申请号：US12301581
申请日：2007-04-17
申请人： Sang Yup Lee , Tae Jung Park
发明人： Sang Yup Lee , Tae Jung Park
IPC分类号： C12N1/20 , C07H21/04
CPC分类号： C07K14/825 , B82Y5/00 , B82Y15/00 , C12N9/104 , C12P3/00 , G01N33/531 , G01N33/588 , Y10T428/2982
摘要： The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.
摘要翻译：本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。更具体地，涉及一种制备重金属结构体的方法，包括以下步骤：在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物，以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物，以及根据所述方法制备的重金属结构纳米颗粒。与通过物理结合金属材料制备量子点的现有方法不同，本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。此外，量子点是有用的，因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式