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1. 发明申请

US20090298706A1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 有权
标题翻译：使用BACILLUS ANTHRACIS EXOSPORIUM的目标蛋白的细胞表面显示方法
公开(公告)号：US20090298706A1
公开(公告)日：2009-12-03
申请号：US12159933
申请日：2006-12-29
申请人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
发明人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
IPC分类号： C40B30/04 , C12N15/63 , C12N1/21 , C12N1/15 , C12N1/19 , C40B50/14
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BclA或其片段的bclA基因的表达载体，并且靶蛋白可以以与BclA或BclA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BclA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

2. 发明授权

US08883692B2 Method for cell surface displaying of target proteins using Bacillus anthracis exosporium 有权
标题翻译：使用炭疽杆菌外芽的细胞表面显示靶蛋白的方法
公开(公告)号：US08883692B2
公开(公告)日：2014-11-11
申请号：US12159933
申请日：2006-12-29
申请人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
发明人： Sang Yup Lee , Tae Jung Park , Nam Su Heo , Jong Hyun Choi
IPC分类号： C40B40/10 , C12P21/02 , C07K16/00 , C07K14/32 , C12N15/70
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BclA或其片段的bclA基因的表达载体，并且靶蛋白可以以与BclA或BclA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BclA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

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