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    • 6. 发明申请
    • METHODS FOR THERAPY OF NEURODEGENERATIVE DISEASE OF THE BRAIN
    • 脑部神经病变疾病治疗方法
    • WO0207774A3
    • 2003-01-16
    • PCT/US0116122
    • 2001-05-17
    • UNIV CALIFORNIATUSZYNSKI MARK H
    • TUSZYNSKI MARK H
    • A61K35/76A61K38/18A61K38/22A61K48/00A61P25/02A61P25/16A61P25/28
    • C12N15/86A61K38/185A61K48/00C12N2750/14143C12N2750/14171
    • A specific clinical protocol for use toward therapy of defective, diseased and damaged cholinergic neurons in the mammalian brain, of particular usefulness for treatment of neurodegenerative conditions such as Alzheimer's disease. The protocol is practiced by delivering a definite concentration of recombinant neurotrophin into, or within close proximity of, identified defective, diseased or damaged brain cells. Using a viral vector, the concentration of neurotrophin delivered as part of a neurotrophic composition varies from 10 to 10 neurotrophin encoding viral particles/ml of composition fluid. Each delivery site receives form 2.5 mu l to 25 mu l of neurotrophic composition, delivered slowly, as in over a period of time ranging upwards of 10 minutes/delivery site. Each delivery site is at, or within 500 mu m of, a targeted cell, and no more than about 10 mm from another delivery site. Stable in situ neurotrophin expression can be achieved for 12 months, or longer.
    • 用于治疗哺乳动物脑中有缺陷的,患病的和受损的胆碱能神经元的特定临床方案,对于治疗神经变性疾病如阿尔茨海默氏病尤其有用。 通过将确定的重组神经营养因子浓度递送到或者非常接近于鉴定的有缺陷的,患病的或受损的脑细胞来实施该方案。 使用病毒载体,作为神经营养组合物一部分递送的神经营养因子的浓度在编码病毒颗粒/ ml组合物流体的10 10至10 15神经营养因子中变化。 每个送货地点收到形式2.5至25微升的神经营养成分,缓慢递送,如超过10分钟/送货地点的一段时间。 每个输送位置在靶细胞的500μm以内,距离另一个递送部位不超过约10mm。 稳定的原位神经营养因子表达可以达到12个月或更长时间。