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1. 发明授权

US5154007A Method and apparatus for cryopreparing biological tissue 失效
标题翻译：冷冻生物组织的方法和装置
公开(公告)号：US5154007A
公开(公告)日：1992-10-13
申请号：US492724
申请日：1990-03-13
申请人： Carmen Piunno , Stephen Livesey , John G. Linner , Anthony A. del Campo , Mark J. Zaltzberg
发明人： Carmen Piunno , Stephen Livesey , John G. Linner , Anthony A. del Campo , Mark J. Zaltzberg
IPC分类号： F26B5/04 , F26B5/06
CPC分类号： F26B5/06 , F26B5/04
摘要： This invention relates to an apparatus and method for distillation drying of one or more biological samples. The drying apparatus includes a retaining assembly, a vacuum assembly, cooling means, monitoring means and control means for actively regulating the temperature and pressure conditions of biological tissue so that such tissue may be dried without substantial ultrastructural damage.
摘要翻译：本发明涉及一种或多种生物样品的蒸馏干燥装置和方法。干燥装置包括保持组件，真空组件，冷却装置，监测装置和用于主动调节生物组织的温度和压力条件的控制装置，使得这样的组织可以被干燥而没有实质的超微结构损伤。

2. 发明申请

US20070078522A2 Particulate Acellular Tissue Matrix 有权
标题翻译：颗粒无细胞组织矩阵
公开(公告)号：US20070078522A2
公开(公告)日：2007-04-05
申请号：US11040127
申请日：2005-01-20
申请人： Edward Griffey , Stephen Livesey , Charles Schiff , Lawrence Boerboom
发明人： Edward Griffey , Stephen Livesey , Charles Schiff , Lawrence Boerboom
IPC分类号： A61F2/02
CPC分类号： A61L27/3804 , A61K9/0024 , A61K9/19 , A61K35/28 , A61K35/32 , A61K35/36 , A61K38/18 , A61K47/42 , A61L27/24 , A61L27/3604 , A61L27/3683 , A61L31/005 , A61L2430/40 , Y10S514/801
摘要： A method of processing an acellular tissue matrix to give a particulate acellular tissue matrix includes: cutting sheets of dry acellular tissue matrix into strips; cryofracturing the dry acellular tissue matrix strips at cryogenic temperatures; separating the resulting particles by size at cryogenic temperatures; and freeze drying the fraction of particles desired size to remove any moisture that may have been absorbed to give a dry particulate acellular tissue matrix. Rehydration of the dry particulate acellular tissue matrix may take place just prior to use. The particulate acellular tissue may be applied to a recipient site, by way of injection, spraying, layering, packing, in-casing or combinations thereof. The particulate acellular tissue may further include growth and stimulating agents selected from epidermal growth factor, fibroblast growth factor, nerve growth factor, keratinocyte growth factor, platelet derived growth factor, vasoactive intestinal peptide, stem cell factor, bone morphogetic proteins, chondrocyte growth factor and combinations thereof. Other pharmaceutically active compounds may be combined with the rehydrated particulate material including: analgesic drugs; hemostatic drugs; antibiotic drugs; local anesthetics and the like to enhance the acceptance of the implanted particulate material. The particulate material product may also be combined with stem cells selected from mesenchymal stem cells, epidermal stem cells, cartilage stem cells, hematopoietic stem cells and combinations thereof.
摘要翻译：一种处理无细胞组织基质以产生颗粒状无细胞组织基质的方法包括：将干燥的无细胞组织基质的片材切成条带; 在低温下对干燥的无细胞组织基质条进行冷冻干燥; 将所得颗粒在低温下分离大小; 并冷冻干燥所需尺寸的颗粒级分以除去可能被吸收的任何水分，以产生干燥的无细胞组织基质。干燥颗粒状无细胞组织基质的再水合可以在使用前进行。颗粒状脱细胞组织可以通过注射，喷雾，分层，包装，套管或其组合施用于受体部位。颗粒细胞组织还可以包括生长和刺激剂，其选自表皮生长因子，成纤维细胞生长因子，神经生长因子，角质形成细胞生长因子，血小板衍生生长因子，血管活性肠肽，干细胞因子，骨形态蛋白，软骨细胞生长因子和其组合。其他药物活性化合物可以与再水合的颗粒材料组合，包括：止痛药; 止血药; 抗生素药物; 局部麻醉剂等，以增强植入颗粒物质的接受性。颗粒材料产品还可以与选自间充质干细胞，表皮干细胞，软骨干细胞，造血干细胞及其组合的干细胞组合。

3. 发明授权

US4707998A Apparatus and method for ultrarapid cooling of biological samples 失效
标题翻译：生物样品超级冷却的装置和方法
公开(公告)号：US4707998A
公开(公告)日：1987-11-24
申请号：US939701
申请日：1986-12-03
申请人： John G. Linner , Stephen Livesey
发明人： John G. Linner , Stephen Livesey
IPC分类号： B01D8/00 , F25D19/00 , G01N1/42 , F25C5/08 , F26B13/30
CPC分类号： B01D8/00 , F25D19/006 , G01N1/42 , F25D2400/30
摘要： A method and apparatus for ultrarapid cooling of tissue samples against a chilled cryogenic surface. The cryogenic surface is enclosed in an ultra-high hydrocarbon free vacuum chamber during cooling of the cryogenic surface by a helium cryopump or multiple-stage refrigerator. Dry helium gas is introduced from an external source to raise the chamber pressure just prior to slamming or plunging a sample against the cryogenic surface. The cryogenic surface is heated for regeneration or cleaning purposes between each successive sample. During heating and cleaning of the cryogenic surface, an evacuated thermal break functionally separates the surface from the helium cryopump or multiple-stage refrigerator used to cool the surface. After regeneration or cleaning the cryogenic surfce, dry helium gas is reintroduced into the thermal break from an external source, thereby enabling the helium cryopump or multiple-stage refrigerator to rapidly recool the cryogenic surface and repeat the cycle for sequential samples with minimal regeneration time between each sample.

4. 发明申请

US20050159822A1 Particulate acellular tissue matrix 有权
标题翻译：颗粒无细胞组织基质
公开(公告)号：US20050159822A1
公开(公告)日：2005-07-21
申请号：US11040127
申请日：2005-01-20
申请人： Edward Griffey , Stephen Livesey , Charles Schiff , Lawrence Boerboom
发明人： Edward Griffey , Stephen Livesey , Charles Schiff , Lawrence Boerboom
IPC分类号： A61K9/00 , A61K9/19 , A61K35/12 , A61K35/28 , A61K35/32 , A61K35/36 , A61K38/18 , A61K47/42 , A61L27/24 , A61L27/36 , A61L27/38 , A61L31/00 , A61F2/02
CPC分类号： A61L27/3804 , A61K9/0024 , A61K9/19 , A61K35/28 , A61K35/32 , A61K35/36 , A61K38/18 , A61K47/42 , A61L27/24 , A61L27/3604 , A61L27/3683 , A61L31/005 , A61L2430/40 , Y10S514/801
摘要： A method of processing an acellular tissue matrix to give a particulate acellular tissue matrix includes: cutting sheets of dry acellular tissue matrix into strips; cryofracturing the dry acellular tissue matrix strips at cryogenic temperatures; separating the resulting particles by size at cryogenic temperatures; and freeze drying the fraction of particles desired size to remove any moisture that may have been absorbed to give a dry particulate acellular tissue matrix. Rehydration of the dry particulate acellular tissue matrix may take place just prior to use. The particulate acellular tissue may be applied to a recipient site, by way of injection, spraying, layering, packing, in-casing or combinations thereof. The particulate acelluar tissue may further include growth and stimulating agents selected from epidermal growth factor, fibroblast growth factor, nerve growth factor, keratinocyte growth factor, platelet derived growth factor, vasoactive intestinal peptide, stem cell factor, bone morphogetic proteins, chondrocyte growth factor and combinations thereof. Other pharmaceutically active compounds may be combined with the rehydrated particulate material including: analgesic drugs; hemostatic drugs; antibiotic drugs; local anesthetics and the like to enhance the acceptance of the implanted particulate material. The particulate material product may also be combined with stem cells selected from mesenchymal stem cells, epidermal stem cells, cartilage stem cells, hematopoietic stem cells and combinations thereof.
摘要翻译：一种处理无细胞组织基质以产生颗粒状无细胞组织基质的方法包括：将干燥的无细胞组织基质的片材切成条带; 在低温下对干燥的无细胞组织基质条进行冷冻干燥; 将所得颗粒在低温下分离大小; 并冷冻干燥所需尺寸的颗粒级分以除去可能被吸收的任何水分，以产生干燥的无细胞组织基质。干燥颗粒状无细胞组织基质的再水合可以在使用前进行。颗粒状脱细胞组织可以通过注射，喷雾，分层，包装，套管或其组合施用于受体部位。颗粒状细丝组织还可以包括生长和刺激剂，其选自表皮生长因子，成纤维细胞生长因子，神经生长因子，角质形成细胞生长因子，血小板衍生生长因子，血管活性肠肽，干细胞因子，骨形态蛋白，软骨细胞生长因子和其组合。其他药物活性化合物可以与再水合的颗粒材料组合，包括：止痛药; 止血药; 抗生素药物; 局部麻醉剂等，以增强植入颗粒物质的接受性。颗粒材料产品还可以与选自间充质干细胞，表皮干细胞，软骨干细胞，造血干细胞及其组合的干细胞组合。

5. 发明申请

US20140186310A1 METHOD OF EX VIVO CELLULAR GROWTH 审中-公开
标题翻译：细胞生长的方法
公开(公告)号：US20140186310A1
公开(公告)日：2014-07-03
申请号：US14122598
申请日：2012-05-25
申请人： Kathy Traianedes , Anthony Stephen Livesey
发明人： Kathy Traianedes , Anthony Stephen Livesey
IPC分类号： A61K35/14
CPC分类号： A61K35/14 , A61K35/28 , A61L27/3633 , A61L27/3834 , A61L27/507
摘要： The present invention relates generally to an ex vivo method of producing a population of cells and materials for use therewith. More particularly, the present invention is directed to an ex vivo method of generating the growth of a population of blood-derived cells and materials for use therewith. The method of the present invention facilitates cell growth by virtue of the migration of blood-derived cells from the vasculature of a vascularised receptacle to the acellular tissue support matrix of said receptacle. These findings have now facilitated the design of means for reliably and efficiently deriving cellular populations from blood-derived cells, such as the generation of bone marrow cells including haemopoietic stem cells and mesenchymal stem cells, for use in a wide variety of clinical and research settings. The method of the present invention is particularly useful for the therapeutic or prophylactic treatment of a range of conditions via the administration of the cells generated in accordance with the method of the present invention.
摘要翻译：本发明一般涉及一种生产与其一起使用的细胞群和材料的离体方法。更具体地说，本发明涉及一种离子生成方法，用于产生血源细胞群和其使用的材料的生长。本发明的方法由于血液源细胞从血管化插管的脉管系统迁移到所述容器的无细胞组织支撑基质而促进细胞生长。这些发现现在有助于设计用于可靠和有效地从血液来源的细胞衍生细胞群体的手段，例如产生包括造血干细胞和间充质干细胞在内的骨髓细胞，用于各种临床和研究环境。本发明的方法特别可用于通过施用根据本发明方法产生的细胞来治疗或预防一系列病症。

6. 发明申请

US20070141703A1 Methods for producing blood products from pluripotent cells in cell culture 审中-公开
标题翻译：在细胞培养中从多能细胞产生血液制品的方法
公开(公告)号：US20070141703A1
公开(公告)日：2007-06-21
申请号：US10579712
申请日：2004-11-19
申请人： Edouard Stanley , Andrew Elefanty , Elizabeth Stadler , Stephen Livesey
发明人： Edouard Stanley , Andrew Elefanty , Elizabeth Stadler , Stephen Livesey
IPC分类号： C12N5/08
CPC分类号： C12N5/0634 , C12N5/0641 , C12N2500/90 , C12N2501/105 , C12N2501/125 , C12N2501/155 , C12N2501/165 , C12N2501/23 , C12N2501/26 , C12N2506/02
摘要： The present invention provides methods for in vitro production of clinically useful quantities of differentiated human blood cells. In various embodiments of the present invention, immortal pluripotent cells are used to produce differentiated blood cell populations using a cell production device. In a specific embodiment, the device is a sequential series of bioreactors utilizing growth media containing specific combinations of maintenance-, proliferation- or differentiation-promoting factors that maintain, expand and promote the maturation and differentiation of the desired cell types. The immortal pluripotent cells can optionally be genetically modified so as to remove histcompatibility or blood group antigens.
摘要翻译：本发明提供了体外产生临床有用量的分化人血细胞的方法。在本发明的各种实施方案中，使用细胞生产装置，使用永生多能细胞产生分化的血细胞群体。在具体实施方案中，该装置是利用包含维持，增殖或分化促进因子的特定组合的生长培养基的连续系列的生物反应器，其维持，扩增和促进所需细胞类型的成熟和分化。永生多能细胞可任选进行遗传修饰，以去除组织相容性或血型抗原。

7. 发明申请

US20060210960A1 Method for processing and preserving collagen-based tissues for transplantation 审中-公开
公开(公告)号：US20060210960A1
公开(公告)日：2006-09-21
申请号：US11177167
申请日：2005-07-07
申请人： Stephen Livesey , Anthony Del Campo , Abhijit Nag , Ken Nichols , Edward Griffey , Christopher Coleman
发明人： Stephen Livesey , Anthony Del Campo , Abhijit Nag , Ken Nichols , Edward Griffey , Christopher Coleman
IPC分类号： A01N1/02 , C12N5/08
CPC分类号： A61L27/3683 , A01N1/00 , A01N1/02 , A01N1/0221 , A01N1/0231 , A61K35/18 , A61K39/12 , A61K39/13 , A61L27/3604 , A61L27/3808 , A61L27/3813 , A61L2430/40 , C12N1/04 , C12N2770/32634 , F25C1/142 , F26B5/065
摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

8. 发明申请

US20060127375A1 Cryopreservation of human red blood cells 审中-公开
标题翻译：人类红细胞的冷冻保存
公开(公告)号：US20060127375A1
公开(公告)日：2006-06-15
申请号：US11334950
申请日：2006-01-18
申请人： Stephen Livesey , Michael Burnett , Jerome Connor , Christopher Wagner
发明人： Stephen Livesey , Michael Burnett , Jerome Connor , Christopher Wagner
IPC分类号： A61K35/14 , A01N1/02 , A61K31/70 , A61K31/7048 , A61K31/522 , A61K31/7012
CPC分类号： A01N1/02 , A01N1/0226 , A61K35/18
摘要： A red blood cell storage composition includes a composition of red blood cells and biochemistry altering reagents, the biochemistry altering reagents being present at a concentration so as to reduce the percent hemolysis of the red blood cells during the freeze-thaw cycle below that of the percent hemolysis of the red blood cells in the absence the biochemistry altering reagents. The red blood cell storage composition preferably includes reagents selected from: modifiers of glycolytic/metabolic components, modifiers of antioxidant potential, effectors of intracellular ionic distribution, modifiers of membrane fluidity, modifiers of cytoskeletal structure, effectors of the cyclooxygenase second messenger pathway, effectors of the lipoxygenase second messenger pathway, effectors of the hexose monophosphate second messenger pathway, effectors of the phosphorylation second messenger pathway, modifiers of specific messenger molecules, and combinations thereof.
摘要翻译：红细胞储存组合物包括红细胞和生物化学改变试剂的组合物，所述生物化学改变试剂以浓度存在，以便将冻融循环期间的红细胞溶血百分比降低至百分比以下在没有生物化学改变试剂的情况下红细胞溶血。红细胞储存组合物优选包括选自以下的试剂：糖酵解/代谢组分的改性剂，抗氧化剂潜力的改性剂，细胞内离子分布的作用剂，膜流动性的调节剂，细胞骨架结构的改性剂，环加氧酶第二信使途径的作用者，脂氧合酶第二信使通路，己糖单磷酸第二信使途径的作用子，磷酸化第二信使途径的作用剂，特异信使分子的改性剂及其组合。

9. 发明授权

US4964280A Method and apparatus for cryopreparing biological tissue 失效
公开(公告)号：US4964280A
公开(公告)日：1990-10-23
申请号：US395028
申请日：1989-08-17
申请人： Carmen Piunno , Stephen Livesey , John G. Linner , Anthony A. del Campo , Mark J. Zaltzberg
发明人： Carmen Piunno , Stephen Livesey , John G. Linner , Anthony A. del Campo , Mark J. Zaltzberg
IPC分类号： F26B5/04 , F26B5/06
CPC分类号： F26B5/04 , F26B5/06
摘要： This invention relates to an apparatus and method for distillation drying of one or more biological samples. The drying apparatus includes a retaining assembly, a vacuum assembly, cooling means, monitoring means and control means for actively regulating the temperature and pressure conditions of biological tissue so that such tissue may be dried without substantial ultrastructural damage.

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