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1. 发明申请

US20090233308A1 NOVEL METHOD OF ASSAYING NUCLEIC ACID USING LABELED NUCLEOTIDE 有权
标题翻译：使用标记核苷酸测定核酸的新方法
公开(公告)号：US20090233308A1
公开(公告)日：2009-09-17
申请号：US12421983
申请日：2009-04-10
申请人： Shinya KURATA , Kyoko Takatsu , Kazunori Nakamura , Takahiro Kanagawa
发明人： Shinya KURATA , Kyoko Takatsu , Kazunori Nakamura , Takahiro Kanagawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2563/107
摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
摘要翻译：提供了一种新的方法来测定至少一种核酸。根据该方法，在核酸聚合反应体系中进行核酸聚合反应，所述核酸聚合反应体系含有（A）至少一种核酸作为模板，（B）至少一种用至少一个选自标记的标记的核苷酸单体由（a）荧光染料，（b）猝灭剂和（c）与其中含有荧光染料或猝灭剂的免疫相关物质组成的组，和（C）至少一种核酸合成酶。然后使用模板核酸作为模板合成的模板核酸或核酸从核酸聚合体系的光学特性的变化或量的变化来测定。该方法使得可以在短时间和容易地特异性且准确地测定包含在单一系统中并且可以是未知核酸和/或已知核酸的至少一种核酸，具有优异的灵敏度。

2. 发明授权

US07354707B2 Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method 失效
标题翻译：核酸探针，通过使用探针测定核酸浓度的方法，以及通过该方法分析数据的方法
公开(公告)号：US07354707B2
公开(公告)日：2008-04-08
申请号：US09891517
申请日：2001-06-27
申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Masaki Torimura , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku
发明人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Masaki Torimura , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku
IPC分类号： C12Q1/68 , C07H21/00 , G06F19/00
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y02A90/26 , C12Q2565/107 , C12Q2565/1015
摘要： Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
摘要翻译：提供核酸探针，每个核酸探针由可与靶核酸杂交并用荧光染料或荧光染料和猝灭剂物质标记的单链寡核苷酸形成。核酸探针可以在短时间内容易地设计，允许测定，多形态分析或核酸的实时定量PCR，并且在反应期间不解离。还提供了使用核酸探针的核酸测定方法，多晶分析方法和实时定量PCR方法。

3. 发明申请

US20070128608A1 Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor 审中-公开
标题翻译：用于测定核酸的新型混合物，使用该核酸的核酸测定新方法和用于其的核酸探针
公开(公告)号：US20070128608A1
公开(公告)日：2007-06-07
申请号：US10583457
申请日：2004-12-20
申请人： Kazunori Nakamura , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
发明人： Kazunori Nakamura , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6818 , Y10T436/143333 , C12Q2545/107
摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.
摘要翻译： [问题]提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 [解决问题的方法] 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

4. 发明授权

US07094540B2 Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method 有权
公开(公告)号：US07094540B2
公开(公告)日：2006-08-22
申请号：US10209608
申请日：2002-08-01
申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
发明人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

5. 发明申请

US20060177856A1 Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method 有权
公开(公告)号：US20060177856A1
公开(公告)日：2006-08-10
申请号：US11340574
申请日：2006-01-27
申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
发明人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

6. 发明授权

US07429655B2 Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method 有权
标题翻译：用于确定靶核酸分子的浓度的方法，用于分析通过该方法得到的数据的方法和方法的核酸探针
公开(公告)号：US07429655B2
公开(公告)日：2008-09-30
申请号：US10683386
申请日：2003-10-14
申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
发明人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
摘要翻译：提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。该方法包括：当与靶核酸杂交时，提供能够减少来自荧光染料的荧光发射的核酸探针作为探针; 将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

7. 发明授权

US07419786B2 Methods for monitoring PCR amplification with fluorescently labeled probes or primers for which fluorescence changes upon hybridization to a target 有权
标题翻译：用荧光标记的探针或引物监测PCR扩增的方法，在与靶标杂交时荧光发生变化
公开(公告)号：US07419786B2
公开(公告)日：2008-09-02
申请号：US11340574
申请日：2006-01-27
申请人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
发明人： Ryuichiro Kurane , Takahiro Kanagawa , Yoichi Kamagata , Shinya Kurata , Kazutaka Yamada , Toyokazu Yokomaku , Osamu Koyama , Kenta Furusho
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
摘要翻译：提供了通过使用用荧光染料标记的核酸探针来测定靶核酸的浓度的方法。该方法包括：当与靶核酸杂交时，提供能够减少来自荧光染料的荧光发射的核酸探针作为探针; 将探针与目标核酸杂交; 并测量在杂交之前相对于来自荧光染料的荧光发射的杂交后荧光染料的荧光发射的减少。

8. 发明申请

US20060172293A1 Novel method of assaying nucleic acid using labeled nucleotide 审中-公开
标题翻译：使用标记核苷酸测定核酸的新方法
公开(公告)号：US20060172293A1
公开(公告)日：2006-08-03
申请号：US10514558
申请日：2003-05-30
申请人： Shinya Kurata , Kyoto Takatsu , Kazunori Nakamura
发明人： Shinya Kurata , Kyoto Takatsu , Kazunori Nakamura
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2563/107
摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
摘要翻译：提供了一种新的方法来测定至少一种核酸。根据该方法，在核酸聚合反应体系中进行核酸聚合反应，所述核酸聚合反应体系含有（A）至少一种核酸作为模板，（B）至少一种用至少一个选自标记的标记的核苷酸单体由（a）荧光染料，（b）猝灭剂和（c）与其中含有荧光染料或猝灭剂的免疫相关物质组成的组，和（C）至少一种核酸合成酶。然后使用模板核酸作为模板合成的模板核酸或核酸从核酸聚合体系的光学特性的变化或量的变化来测定。该方法使得可以在短时间和容易地特异性且准确地测定包含在单一系统中并且可以是未知核酸和/或已知核酸的至少一种核酸，具有优异的灵敏度。

9. 发明授权

US07951604B2 Mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor 有权
标题翻译：用于测定核酸的混合物，使用该核酸测定核酸的新方法和用于其的核酸探针
公开(公告)号：US07951604B2
公开(公告)日：2011-05-31
申请号：US12540390
申请日：2009-08-13
申请人： Kazunori Nakamura , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
发明人： Kazunori Nakamura , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
IPC分类号： G01N33/00 , G01N21/76 , C07H21/04
CPC分类号： C12Q1/6818 , Y10T436/143333 , C12Q2545/107
摘要： To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.
摘要翻译：提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

10. 发明授权

US07785795B2 Method of assaying nucleic acid using labeled nucleotide 有权
标题翻译：使用标记核苷酸测定核酸的方法
公开(公告)号：US07785795B2
公开(公告)日：2010-08-31
申请号：US12421983
申请日：2009-04-10
申请人： Shinya Kurata , Kyoko Takatsu , Kazunori Nakamura , Takahiro Kanagawa
发明人： Shinya Kurata , Kyoko Takatsu , Kazunori Nakamura , Takahiro Kanagawa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q2563/107
摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
摘要翻译：提供了一种新的方法来测定至少一种核酸。根据该方法，在核酸聚合反应体系中进行核酸聚合反应，所述核酸聚合反应体系含有（A）至少一种核酸作为模板，（B）至少一种用至少一个选自标记的标记的核苷酸单体由（a）荧光染料，（b）猝灭剂和（c）与其中含有荧光染料或猝灭剂的免疫相关物质组成的组，和（C）至少一种核酸合成酶。然后使用模板核酸作为模板合成的模板核酸或核酸从核酸聚合体系的光学特性的变化或量的变化来测定。该方法使得可以在短时间和容易地特异性且准确地测定包含在单一系统中并且可以是未知核酸和/或已知核酸的至少一种核酸，具有优异的灵敏度。

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