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1. 发明申请

US20060172293A1 Novel method of assaying nucleic acid using labeled nucleotide 审中-公开
标题翻译：使用标记核苷酸测定核酸的新方法
公开(公告)号：US20060172293A1
公开(公告)日：2006-08-03
申请号：US10514558
申请日：2003-05-30
申请人： Shinya Kurata , Kyoto Takatsu , Kazunori Nakamura
发明人： Shinya Kurata , Kyoto Takatsu , Kazunori Nakamura
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2563/107
摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
摘要翻译：提供了一种新的方法来测定至少一种核酸。根据该方法，在核酸聚合反应体系中进行核酸聚合反应，所述核酸聚合反应体系含有（A）至少一种核酸作为模板，（B）至少一种用至少一个选自标记的标记的核苷酸单体由（a）荧光染料，（b）猝灭剂和（c）与其中含有荧光染料或猝灭剂的免疫相关物质组成的组，和（C）至少一种核酸合成酶。然后使用模板核酸作为模板合成的模板核酸或核酸从核酸聚合体系的光学特性的变化或量的变化来测定。该方法使得可以在短时间和容易地特异性且准确地测定包含在单一系统中并且可以是未知核酸和/或已知核酸的至少一种核酸，具有优异的灵敏度。

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