专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060292667A1 Method for improving solubility and folding efficiency of target proteins using RNA as molecular chaperone 有权
标题翻译：使用RNA作为分子伴侣来提高靶蛋白的溶解度和折叠效率的方法
公开(公告)号：US20060292667A1
公开(公告)日：2006-12-28
申请号：US11482401
申请日：2006-07-07
申请人： Baik Seong , Seong-Il Choi , Hang Shin , Ki-Sun Ryu , Kyoung Han , Chul Kim , Byung Kim
发明人： Baik Seong , Seong-Il Choi , Hang Shin , Ki-Sun Ryu , Kyoung Han , Chul Kim , Byung Kim
IPC分类号： C07H21/02 , C12P21/06 , C07K14/47
CPC分类号： C07H21/04 , C07H21/02 , C07K2319/35 , C07K2319/85 , C12N15/62 , C12P21/02
摘要： Disclosed is a method for improving folding efficiency and solubility of a target protein linked to a RNA-binding protein by using RNA molecule as a molecular chaperone, wherein the RNA molecule interacts with the RNA-binding protein. More particularly, the present invention discloses method for improving folding efficiency and solubility of a target protein by transformation of a host cell with a expression vector comprising a polynucleotide encoding the target protein linked to an RNA-binding protein; culturing the transformed host cell in an appropriate culture medium under the condition that an RNA molecule either resident inside the host cell or provided by cotransformation of the host cell with polynucleotide encoding the RNA molecule interacts with the RNA-binding protein; and purifying the soluble protein from host cell lysate. The method of the present invention is very useful for production of soluble proteins for therapeutic, prophylactic and diagnostic applications.
摘要翻译：公开了通过使用RNA分子作为分子伴侣来提高与RNA结合蛋白质连接的靶蛋白的折叠效率和溶解度的方法，其中RNA分子与RNA结合蛋白相互作用。更具体地，本发明公开了通过用包含编码与RNA结合蛋白连接的靶蛋白的多核苷酸的表达载体转化宿主细胞来提高靶蛋白的折叠效率和溶解度的方法。在宿主在宿主细胞内的RNA分子或通过宿主细胞与编码RNA分子的多核苷酸共转化的RNA分子与RNA结合蛋白相互作用的条件下，在适当的培养基中培养转化的宿主细胞; 并从宿主细胞裂解液纯化可溶性蛋白质。本发明的方法对于用于治疗，预防和诊断应用的可溶性蛋白质的生产非常有用。

2. 发明申请

US20120224762A1 METHOD AND APPARATUS FOR OBTAINING PANORAMIC IMAGES 有权
标题翻译：用于获取全景图像的方法和装置
公开(公告)号：US20120224762A1
公开(公告)日：2012-09-06
申请号：US13509042
申请日：2010-11-10
申请人： Seong-Il Choi , Hyo Sung Cho
发明人： Seong-Il Choi , Hyo Sung Cho
IPC分类号： G06K9/00
CPC分类号： H04N5/32 , A61B6/14 , A61B6/488 , A61B6/542 , A61B6/545 , H04N5/232 , H04N5/23238
摘要： The present invention provides an apparatus and a method for obtaining panoramic images in which cervical effect is corrected in such a manner that the uniform contrast is shown. According to the apparatus and the method, the method comprises the steps of: obtaining a preceding frame image by irradiating radiation on one certain section of an image layer trace on which the tooth trace of a subject is embodied; calculating a gray scale average value of pixels that are included within a predetermined reference region of the preceding frame image; comparing the calculated gray scale average value with a gray scale reference value that is calculated from a prepared sample image, and generating a radiation correction value corresponding to a difference value therebetween; and obtaining a succeeding frame image by irradiating strength-adjusted radiation on one section that is adjacent to the other section of an unknown layer trace, according to the calculated radiation correction value.
摘要翻译：本发明提供一种用于获得全景图像的装置和方法，其中以示出均匀对比度的方式校正子宫颈效果。根据该装置和方法，该方法包括以下步骤：通过在其上体现对象的牙迹的图像层迹线的一个特定部分上照射辐射来获得前一帧图像; 计算包括在前一帧图像的预定参考区域内的像素的灰度级平均值; 将所计算的灰度平均值与从准备的样本图像计算出的灰度参考值进行比较，并产生与其之间的差值对应的辐射校正值; 并且根据所计算的辐射校正值，通过对与未知层迹线的另一部分相邻的一个部分照射强度调节的辐射来获得后续帧图像。

3. 发明授权

US07759303B2 Method for improving solubility and folding efficiency of target proteins using RNA as molecular chaperone 有权
标题翻译：使用RNA作为分子伴侣来提高靶蛋白的溶解度和折叠效率的方法
公开(公告)号：US07759303B2
公开(公告)日：2010-07-20
申请号：US11482401
申请日：2006-07-07
申请人： Baik Lin Seong , Seong-Il Choi , Hang Cheol Shin , Ki-Sun Ryu , Kyoung Sim Han , Chul Woo Kim , Byung Hee Kim
发明人： Baik Lin Seong , Seong-Il Choi , Hang Cheol Shin , Ki-Sun Ryu , Kyoung Sim Han , Chul Woo Kim , Byung Hee Kim
IPC分类号： C07K14/00 , C07H21/02 , C12N15/00
CPC分类号： C07H21/04 , C07H21/02 , C07K2319/35 , C07K2319/85 , C12N15/62 , C12P21/02
摘要： Disclosed is a method for improving folding efficiency and solubility of a target protein linked to a RNA-binding protein by using RNA molecule as a molecular chaperone, wherein the RNA molecule interacts with the RNA-binding protein. More particularly, the present invention discloses method for improving folding efficiency and solubility of a target protein by transformation of a host cell with a expression vector comprising a polynucleotide encoding the target protein linked to an RNA-binding protein; culturing the transformed host cell in an appropriate culture medium under the condition that an RNA molecule either resident inside the host cell or provided by cotransformation of the host cell with polynucleotide encoding the RNA molecule interacts with the RNA-binding protein; and purifying the soluble protein from host cell lysate. The method of the present invention is very useful for production of soluble proteins for therapeutic, prophylactic and diagnostic applications.
摘要翻译：公开了通过使用RNA分子作为分子伴侣来提高与RNA结合蛋白质连接的靶蛋白的折叠效率和溶解度的方法，其中RNA分子与RNA结合蛋白相互作用。更具体地，本发明公开了通过用包含编码与RNA结合蛋白连接的靶蛋白的多核苷酸的表达载体转化宿主细胞来提高靶蛋白的折叠效率和溶解度的方法。在宿主在宿主细胞内的RNA分子或通过宿主细胞与编码RNA分子的多核苷酸共转化的RNA分子与RNA结合蛋白相互作用的条件下，在适当的培养基中培养转化的宿主细胞; 并从宿主细胞裂解液纯化可溶性蛋白质。本发明的方法对于用于治疗，预防和诊断应用的可溶性蛋白质的生产非常有用。

4. 发明授权

US06852512B2 Expression vectors for production of foreign proteins as soluble forms 失效
标题翻译：用于产生外源蛋白质作为可溶形式的表达载体
公开(公告)号：US06852512B2
公开(公告)日：2005-02-08
申请号：US09974248
申请日：2001-10-09
申请人： Seong Il Choi , Baik Lin Seong
发明人： Seong Il Choi , Baik Lin Seong
IPC分类号： C07K14/53 , C07K14/81 , C12N9/00 , C12N15/62 , C12P21/04 , C12P21/06 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12N9/93 , C07K14/53 , C07K14/8146 , C07K2319/00 , C07K2319/21 , C07K2319/35 , C07K2319/50 , C07K2319/75 , C12N15/625
摘要： The present invention relates to novel expression vectors which can produce foreign proteins as soluble forms by using lysyl-tRNA synthetase and a process for preparing foreign proteins by using the expression vectors. Particularly, the present invention relates to the expression vectors which can provide foreign proteins as fused and soluble forms by exploiting the structure and expression pattern of lysyl-tRNA synthetase and the processes for preparing foreign proteins in E. coli effectively, which can be utilized industrially to produce active proteins in mass.
摘要翻译：本发明涉及可通过使用赖氨酰-tRNA合成酶产生外源蛋白作为可溶性形式的新型表达载体和通过使用表达载体制备外源蛋白的方法。特别地，本发明涉及通过利用赖氨酰-tRNA合成酶的结构和表达模式以及在大肠杆菌中有效制备外源蛋白的方法，可以提供外来蛋白质作为融合和可溶性形式的表达载体，其可以在工业上使用以产生大量的活性蛋白质。

5. 发明授权

US06393188B1 Dispersion compensation device and optical transmission system with the same 有权
标题翻译：色散补偿装置与光传输系统相同
公开(公告)号：US06393188B1
公开(公告)日：2002-05-21
申请号：US09388488
申请日：1999-09-02
申请人： Ki-Tae Jeong , Young-Tark Lee , Ho-Jin Jeong , Han-Kyo Seo , Jeong-U Jeon , Tae-Sang Park , Seok-Bong Ko , Yun-Hee Cho , Seong-Il Choi , Sang-Woork Park
发明人： Ki-Tae Jeong , Young-Tark Lee , Ho-Jin Jeong , Han-Kyo Seo , Jeong-U Jeon , Tae-Sang Park , Seok-Bong Ko , Yun-Hee Cho , Seong-Il Choi , Sang-Woork Park
IPC分类号： G02B616
CPC分类号： G02B6/29377 , G02B6/29394 , G02B6/4215
摘要： A dispersion compensation device and an optical transmission system are capable of simultaneously compensating a dispersion value and dispersion slope in a single-mode optical fiber in a high-speed long-distance optical transmission system and a wavelength division optical transmission system. The dispersion compensation device includes N number of component optical fibers arranged in a serial fashion, N being a positive integer more than one, wherein each of the component optical fiber has a different dispersion value per a unit length, a dispersion slope per a unit length, and a different length.
摘要翻译：色散补偿装置和光传输系统能够同时补偿高速长距离光传输系统和波分分光传输系统中的单模光纤中的色散值和色散斜率。色散补偿装置包括以串联方式布置的N个分量光纤，N是大于1的正整数，其中每个分量光纤每单位长度具有不同的色散值，每单位长度的色散斜率，和不同的长度。

6. 发明授权

US06381226B1 Guard time reducing system in data communication from earth station to static orbit communication satellite and method thereof 失效
标题翻译：从地球站到静态轨道通信卫星的数据通信中的保卫时间减少系统及其方法
公开(公告)号：US06381226B1
公开(公告)日：2002-04-30
申请号：US09188800
申请日：1998-11-09
申请人： Seong Il Choi
发明人： Seong Il Choi
IPC分类号： H04B7185
CPC分类号： H04B7/2125
摘要： A guard time reducing system and a method thereof for realizing a guard time that each packet is near each other in time without overlapping each other. A GPS timing unit finds the propagation passed time from a static orbit communication satellite to an earth station and a packetizing unit transmits a packet as fast as the propagation passed time than the UTC time when the packetizing unit transmits the packet to the static orbit communication satellite. According to the method, it is possible to maximize the use efficiency of the communication channel from the earth station to the static orbit communication satellite because respective earth station transmits data in each time period allocated thereto without overlapping each time period.
摘要翻译：一种保护时间减少系统及其方法，用于实现每个分组在时间上彼此接近而彼此不重叠的保护时间。 GPS定时单元从静态轨道通信卫星到地球站发现传播时间的传播，并且分组单元发送与传播分组到静态轨道通信卫星的UTC时间相比传播时间快的分组。根据该方法，由于各地球站在分配给其的每个时间段中发送数据而不重叠每个时间段，所以可以使从地球站到静态轨道通信卫星的通信信道的使用效率最大化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式