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1. 发明申请

WO2003002979A3 MULTIPLEX DECODING OF ARRAY SENSORS WITH MICROSPHERES 审中-公开
公开(公告)号：WO2003002979A3
公开(公告)日：2003-01-09
申请号：PCT/US2002/022095
申请日：2002-06-28
申请人： ILLUMINA, INC. , CHEE, Mark, S. , STUELPNAGEL, John, R. , CZARNIK, Anthony, W.
发明人： CHEE, Mark, S. , STUELPNAGEL, John, R. , CZARNIK, Anthony, W.
IPC分类号： C12Q1/68
摘要： The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.

2. 发明申请

WO2002101358A3 MULTIPLEXED DETECTION METHODS 审中-公开
公开(公告)号：WO2002101358A3
公开(公告)日：2002-12-19
申请号：PCT/US2002/018634
申请日：2002-06-11
申请人： ILLUMINA, INC. , FAN, Jian-Bing , STUELPNAGEL, John, R. , CHEE, Mark, S.
发明人： FAN, Jian-Bing , STUELPNAGEL, John, R. , CHEE, Mark, S.
IPC分类号： C12P19/34
摘要： The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.

3. 发明申请

WO2002021128A3 CELLULAR ARRAYS COMPRISING ENCODED CELLS 审中-公开
公开(公告)号：WO2002021128A3
公开(公告)日：2002-03-14
申请号：PCT/US2001/027510
申请日：2001-09-05
申请人： ILLUMINA, INC. , STUELPNAGEL, John, R.
发明人： STUELPNAGEL, John, R.
IPC分类号： G01N33/50
摘要： A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large number of individual cell respnses to target analyses. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner. The biosensor array and measurement method may be employed in the study of biologically active materials in situ environmental monitoring, monitoring of a variety of bioprocesses, and for high throughput screening of large combinatorial chemical libraries.

4. 发明申请

WO2008045158A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION 审中-公开
标题翻译：使用杂化代表性选择核酸FRO复合物的组合物和方法
公开(公告)号：WO2008045158A1
公开(公告)日：2008-04-17
申请号：PCT/US2007/018687
申请日：2007-08-24
申请人： ILLUMINA, INC. , STUELPNAGEL, John, R. , BARKER, David, L. , VELARDE, Jorge, Jr. , BARNARD, Steven, M. , GRAIGE, Michael
发明人： STUELPNAGEL, John, R. , BARKER, David, L. , VELARDE, Jorge, Jr. , BARNARD, Steven, M. , GRAIGE, Michael
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译：本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同复杂混合物中更多的核酸。还提供了从完整基因组中选择基因组序列的表示样品的方法。本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于复制中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。

5. 发明申请

WO2004001062A2 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
标题翻译：多重核酸反应
公开(公告)号：WO2004001062A2
公开(公告)日：2003-12-31
申请号：PCT/US0319680
申请日：2003-06-20
申请人： ILLUMINA INC , SHEN MIN-JUI RICHARD , OLIPHANT ARNOLD , BUTLER SCOTT L , STUELPNAGEL JOHN R , CHEE MARK S , KUHN KENNETH M , FAN JIAN-BING
发明人： SHEN MIN-JUI RICHARD , OLIPHANT ARNOLD , BUTLER SCOTT L , STUELPNAGEL JOHN R , CHEE MARK S , KUHN KENNETH M , FAN JIAN-BING
IPC分类号： C12P19/34 , C12Q1/68 , C12Q
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2535/125 , C12Q2537/143 , C12Q2561/125 , C12Q2565/515
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

6. 发明申请

WO0221128A2 CELLULAR ARRAYS COMPRISING ENCODED CELLS 审中-公开
标题翻译：包含编码细胞的细胞阵列
公开(公告)号：WO0221128A2
公开(公告)日：2002-03-14
申请号：PCT/US0127510
申请日：2001-09-05
申请人： ILLUMINA INC , STUELPNAGEL JOHN R
发明人： STUELPNAGEL JOHN R
IPC分类号： C07B61/00 , G01N33/50 , G01N33/543
CPC分类号： G01N33/502 , C40B40/00 , G01N33/5008 , G01N33/54373
摘要： A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large number of individual cell respnses to target analyses. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner. The biosensor array and measurement method may be employed in the study of biologically active materials in situ environmental monitoring, monitoring of a variety of bioprocesses, and for high throughput screening of large combinatorial chemical libraries.
摘要翻译：提供了生物传感器，传感器阵列，感测方法和感测装置，其中对化学和生物材料具有独特响应特性的单个细胞或随机混合的细胞群体部署在基底上的多个离散位置。在优选实施例中，离散位置包括在光纤阵列内的各个纤维的远端处形成的微孔。生物传感器阵列利用光学可询问编码方案来确定阵列中每种细胞类型的身份和位置，并提供大量单个细胞反应同时测量以进行目标分析。感测方法利用细胞群体以特征和可检测方式对生物学重要化合物的独特能力。生物传感器阵列和测量方法可用于研究生物活性材料的原位环境监测，各种生物过程的监测以及大量组合化学文库的高通量筛选。

7. 发明申请

WO2004001062A3 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
公开(公告)号：WO2004001062A3
公开(公告)日：2003-12-31
申请号：PCT/US2003/019680
申请日：2003-06-20
申请人： ILLUMINA, INC. , SHEN, Min-Jui, Richard , OLIPHANT, Arnold , BUTLER, Scott, L. , STUELPNAGEL, John, R. , CHEE, Mark, S. , KUHN, Kenneth, M. , FAN, Jian-Bing
发明人： SHEN, Min-Jui, Richard , OLIPHANT, Arnold , BUTLER, Scott, L. , STUELPNAGEL, John, R. , CHEE, Mark, S. , KUHN, Kenneth, M. , FAN, Jian-Bing
IPC分类号： C12P19/34
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

8. 发明申请

WO02101358A9 MULTIPLEXED DETECTION METHODS 审中-公开
标题翻译：多重检测方法
公开(公告)号：WO02101358A9
公开(公告)日：2003-03-27
申请号：PCT/US0218634
申请日：2002-06-11
申请人： ILLUMINA INC , FAN JIAN-BING , STUELPNAGEL JOHN R , CHEE MARK S
发明人： FAN JIAN-BING , STUELPNAGEL JOHN R , CHEE MARK S
IPC分类号： C12Q1/68 , G01N
CPC分类号： C12Q1/6809 , C12Q2563/179 , C12Q2525/15
摘要： The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.
摘要翻译：本发明允许样品中靶分子的高度多重检测。该方法包括使目标分析物与包含扩增酶和第一和第二靶标探针的组合物接触。第一和第二靶探针分别包含特异性结合第一和第二靶分子的第一和第二生物活性剂。探针还可以分别包含第一和第二衔接子序列，并且第一衔接子序列分别标识第一靶分子，第二衔接子序列分别标识第二靶分子以及第一和部分上游通用引物序列。最后，扩增后，该方法包括检测第一和第二扩增子，由此分别检测第一和第二靶分子。

9. 发明申请

WO2003002979A2 MULTIPLEX DECODING OF ARRAY SENSORS WITH MICROSPHERES 审中-公开
标题翻译：用微球对阵列传感器进行多重解码
公开(公告)号：WO2003002979A2
公开(公告)日：2003-01-09
申请号：PCT/US2002/022095
申请日：2002-06-28
申请人： ILLUMINA, INC. , CHEE, Mark, S. , STUELPNAGEL, John, R. , CZARNIK, Anthony, W.
发明人： CHEE, Mark, S. , STUELPNAGEL, John, R. , CZARNIK, Anthony, W.
IPC分类号： G01N
CPC分类号： G01N33/54313 , B01J2219/005 , B01J2219/00576 , C12Q1/6816 , C12Q1/6834 , C12Q1/6837 , C12Q2563/179 , C12Q2563/155 , C12Q2563/107
摘要： The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.
摘要翻译：
本发明涉及用于微球阵列传感器的多路解码的组合物和方法。

10. 发明申请

WO2002101358A2 MULTIPLEXED DETECTION METHODS 审中-公开
标题翻译：多重检测方法
公开(公告)号：WO2002101358A2
公开(公告)日：2002-12-19
申请号：PCT/US2002/018634
申请日：2002-06-11
申请人： ILLUMINA, INC. , FAN, Jian-Bing , STUELPNAGEL, John, R. , CHEE, Mark, S.
发明人： FAN, Jian-Bing , STUELPNAGEL, John, R. , CHEE, Mark, S.
IPC分类号： G01N
CPC分类号： C12Q1/6809 , C12Q2563/179 , C12Q2525/15
摘要： The present invention is directed to sensitive and accurate multiplexed assays for target analyte detection.
摘要翻译：本发明允许样品中靶分子的高度多重检测。该方法包括使目标分析物与包含扩增酶和第一和第二靶标探针的组合物接触。第一和第二靶探针分别包含特异性结合第一和第二靶分子的第一和第二生物活性剂。探针还可以分别包含第一和第二衔接子序列，并且第一衔接子序列分别标识第一靶分子，第二衔接子序列分别标识第二靶分子以及第一和部分上游通用引物序列。最后，扩增后，该方法包括检测第一和第二扩增子，由此分别检测第一和第二靶分子。

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