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1. 发明专利

IL234865A Kinetic exclusion amplification of nucleic acid libraries 未知
公开(公告)号：IL234865A
公开(公告)日：2020-10-29
申请号：IL23486514
申请日：2014-09-29
申请人： ILLUMINA INC , SHEN MIN JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
发明人： SHEN MIN-JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
IPC分类号： B01J19/00 , C12Q1/68 , C12Q1/6844 , C12Q1/6848 , C12Q1/6874
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

2. 发明专利

IL278008D0 Kinetic exclusion amplification of nucleic acid libraries 未知
公开(公告)号：IL278008D0
公开(公告)日：2020-11-30
申请号：IL27800820
申请日：2020-10-13
申请人： ILLUMINA INC , SHEN MIN JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
发明人： SHEN MIN-JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
IPC分类号： B01J19/00 , C12Q1/6844 , C12Q1/6848 , C12Q1/6874
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

3. 发明专利

IL278008A Kinetic exclusion amplification of nucleic acid libraries 未知
公开(公告)号：IL278008A
公开(公告)日：2021-12-01
申请号：IL27800820
申请日：2020-10-13
申请人： ILLUMINA INC , SHEN MIN JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
发明人： SHEN MIN-JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
IPC分类号： B01J19/00
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

4. 发明专利

IL234865D0 Kinetic exclusion amplification of nucleic acid libraries 未知
公开(公告)号：IL234865D0
公开(公告)日：2014-12-31
申请号：IL23486514
申请日：2014-09-29
申请人： ILLUMINA INC , SHEN MIN JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
发明人： SHEN MIN-JUI RICHARD , BOUTELL JONATHAN MARK , RONAGHI MOSTAFA , GUNDERSON KEVIN L , VENKATESAN BALA MURALI , BOWEN M SHANE
IPC分类号： C12Q20060101
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

5. 发明申请

WO2013044018A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸序列的方法和组合
公开(公告)号：WO2013044018A1
公开(公告)日：2013-03-28
申请号：PCT/US2012/056556
申请日：2012-09-21
申请人： ILLUMINA, INC. , KAIN, Robert C. , LIU, Xiaohai , FENG, Wenyi , HIRSCHBEIN, Bernard , ELTOUKHY, Helmy A. , WU, Xiaolin , SMITH, Geoffrey Paul , BOUTELL, Jonathan Mark , JOSEPH, Thomas , SMITH, Randall , SHEN, Min-Jui Richard , TREGIDGO, Carolyn , KLAUSING, Kay
发明人： KAIN, Robert C. , LIU, Xiaohai , FENG, Wenyi , HIRSCHBEIN, Bernard , ELTOUKHY, Helmy A. , WU, Xiaolin , SMITH, Geoffrey Paul , BOUTELL, Jonathan Mark , JOSEPH, Thomas , SMITH, Randall , SHEN, Min-Jui Richard , TREGIDGO, Carolyn , KLAUSING, Kay
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
摘要翻译：本公开提供了用于检测样品中多个不同核苷酸的方法和系统。具体地，本公开提供了使用比检测到的核苷酸数目少于检测到的核苷酸数目和/或比检测到的核苷酸数目更少的成像事件来检测样品中的多个不同核苷酸。

6. 发明申请

WO2012106081A3 METHODS FOR REDUCING NUCLEIC ACID DAMAGE 审中-公开
标题翻译：减少核酸损伤的方法
公开(公告)号：WO2012106081A3
公开(公告)日：2012-11-01
申请号：PCT/US2012021040
申请日：2012-01-12
申请人： ILLUMINA INC , ILLUMINA CAMBRIDGE LTD , KLAUSING KAY , SHEN MIN-JUI RICHARD , MOORE JOHN , SMITH VINCENT , HALL KEVIN , GORMLEY NIALL ANTHONY , IOANNOU AVGOUSTA , FRITZILAS EPAMEINONDAS , RIGATTI ROBERTO
发明人： KLAUSING KAY , SHEN MIN-JUI RICHARD , MOORE JOHN , SMITH VINCENT , HALL KEVIN , GORMLEY NIALL ANTHONY , IOANNOU AVGOUSTA , FRITZILAS EPAMEINONDAS , RIGATTI ROBERTO
IPC分类号： C12Q1/68 , C12Q1/48 , G01N33/53
CPC分类号： C12P19/34 , C12N15/1003 , C12Q1/6806 , C12Q2521/531 , C12Q2527/125 , C12Q2563/131
摘要： Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.
摘要翻译：本文提供了在选自片段化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中接触抑制核酸的降解。本文还提供了抑制核酸的光诱导降解的方法。另外，本文提供了在制备核酸样品期间减少或抑制核酸损伤的方法，其包括在包含一种或多种化合物的溶液中使样品中的核酸序列片段化，所述化合物抑制核酸样品中核酸序列降解样品。

7. 发明申请

WO2011025477A1 METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 审中-公开
标题翻译：选择和放大多核苷酸的方法
公开(公告)号：WO2011025477A1
公开(公告)日：2011-03-03
申请号：PCT/US2009/054945
申请日：2009-08-25
申请人： ILLUMINA, INC. , SABOT, Andrea , RIGATTI, Roberto , SHEN, Min-jui, Richard
发明人： SABOT, Andrea , RIGATTI, Roberto , SHEN, Min-jui, Richard
IPC分类号： C12P19/34
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

8. 发明申请

WO2010068702A3 METHODS AND COMPOSITIONS FOR HYBRIDIZING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2010068702A3
公开(公告)日：2010-06-17
申请号：PCT/US2009/067392
申请日：2009-12-09
申请人： ILLUMINA, INC. , YEAKLEY, Joanne, M. , LENTA, Ross, Edward , SHEN, Min-jui, Richard
发明人： YEAKLEY, Joanne, M. , LENTA, Ross, Edward , SHEN, Min-jui, Richard
IPC分类号： C12Q1/68 , C12N15/10
摘要： Methods and compositions for hybridizing nucleic acids are disclosed herein. Also disclosed herein are methods and compositions to detect a nucleic acid provided to an array.

9. 发明申请

WO2012106081A2 METHODS FOR REDUCING NUCLEIC ACID DAMAGE 审中-公开
标题翻译：降低核酸损伤的方法
公开(公告)号：WO2012106081A2
公开(公告)日：2012-08-09
申请号：PCT/US2012/021040
申请日：2012-01-12
申请人： ILLUMINA, INC. , ILLUMINA CAMBRIDGE LTD. , KLAUSING, Kay , SHEN, Min-Jui Richard , MOORE, John , SMITH, Vincent , HALL, Kevin , GORMLEY, Niall Anthony , IOANNOU, Avgousta , FRITZILAS, Epameinondas , RIGATTI, Roberto
发明人： KLAUSING, Kay , SHEN, Min-Jui Richard , MOORE, John , SMITH, Vincent , HALL, Kevin , GORMLEY, Niall Anthony , IOANNOU, Avgousta , FRITZILAS, Epameinondas , RIGATTI, Roberto
IPC分类号： C12Q1/68 , C12Q1/48 , G01N33/53
CPC分类号： C12P19/34 , C12N15/1003 , C12Q1/6806 , C12Q2521/531 , C12Q2527/125 , C12Q2563/131
摘要： Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.
摘要翻译：本文提供了一种在选择片段化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中所述接触抑制核酸的降解。本文还提供抑制光诱导的核酸降解的方法。此外，本文提供的是在制备核酸样品期间减少或抑制核酸损伤的方法，包括在包含一种或多种化合物的溶液中碎片样品中的核酸序列，所述化合物抑制核酸序列的降解样品。

10. 发明申请

WO2011106368A3 AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS 审中-公开
标题翻译：最小化序列特异性偏差的放大方法
公开(公告)号：WO2011106368A3
公开(公告)日：2012-01-19
申请号：PCT/US2011025851
申请日：2011-02-23
申请人： ILLUMINA INC , RIGATTI ROBERTO , BOUTELL JONATHAN MARK , SHEN MIN-JUI RICHARD
发明人： RIGATTI ROBERTO , BOUTELL JONATHAN MARK , SHEN MIN-JUI RICHARD
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells
摘要翻译：提供了扩增核酸的方法。这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。该方法适用于固相扩增，例如利用流动池

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