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1. 发明申请

WO2010129279A3 ELECTROADDRESSING AND IN-FILM BIOPROCESSING USING STIMULI-RESPONSIVE HYDROGEL-FORMING POLYMERS 审中-公开
标题翻译：使用刺激性水凝胶形成聚合物进行电加工和膜内生物处理
公开(公告)号：WO2010129279A3
公开(公告)日：2011-05-05
申请号：PCT/US2010032575
申请日：2010-04-27
申请人： UNIV MARYLAND BIOTECH INST , SHI XIAO-WEN , LIU YI , PAYNE GREGORY F , YANG XIAOHUA
发明人： SHI XIAO-WEN , LIU YI , PAYNE GREGORY F , YANG XIAOHUA
IPC分类号： C12N11/04 , C08B37/04 , C08B37/12
CPC分类号： C12N11/04 , B01J2219/00576 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00725 , B01J2219/00743 , C09D105/04 , G01N33/5438
摘要： Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.
摘要翻译：描述了通过电解可聚合物的电沉积形成的水凝胶的产生方法。水凝胶可以含有一个或多个电解地址或电可寻址到水凝胶内的位置的细胞群，并且其中细胞群的细胞被水凝胶包埋并且能够在水凝胶内膨胀并且可以从水凝胶中释放。还提供了用于细胞群的膜内扩增的电可寻址多糖共混物，允许细胞的探查和免疫复合物的形成。还提供了在膜内生物处理方法如基于细胞的生物传感，基于蛋白质的生物传感和细胞信号传导研究中使用含有电解或电可寻址细胞群的水凝胶的方法。

2. 发明申请

WO2010040047A3 CHITOSAN-COATED WIRES FOR BIOSENSING 审中-公开
标题翻译：用于生物传感器的壳聚糖涂层电线
公开(公告)号：WO2010040047A3
公开(公告)日：2010-07-22
申请号：PCT/US2009059372
申请日：2009-10-02
申请人： UNIV MARYLAND BIOTECH INST , LIU YI , SHI XIAO-WEN , PAYNE GREGORY F , MEYER W LEE
发明人： LIU YI , SHI XIAO-WEN , PAYNE GREGORY F , MEYER W LEE
IPC分类号： G01N33/50
CPC分类号： G01N33/54393
摘要： A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electrodepositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.
摘要翻译：一种在导电基底上形成包括蛋白质的生物电子装置的方法，通过在基底上电沉积氨基多糖脱乙酰壳多糖，同时对基底施加阴极电压，在其上形成氨基多糖脱乙酰壳多糖膜，在存在的情况下向基底施加阳极电压的NaCl活化氨基多糖壳聚糖膜，使其与蛋白质反应。该方法还任选地包括使氨基多糖膜在其活化后与蛋白质反应，以使得蛋白质聚集并耦合至基底，由此形成生物电子装置。蛋白质可以包括单个或多个蛋白质种类，并且包括生物传感蛋白质。另外的方法包括对存在于样品中或在生物学识别事件期间产生的电化学活性化合物的生物传感以及在这些方法中有用的装置。所得到的装置可用作手持装置，纺织品，服装等中的传感器。

3. 发明申请

WO2010129279A2 ELECTROADDRESSING AND IN-FILM BIOPROCESSING USING STIMULI-RESPONSIVE HYDROGEL-FORMING POLYMERS 审中-公开
标题翻译：使用刺激性水凝胶形成聚合物进行电加工和膜内生物处理
公开(公告)号：WO2010129279A2
公开(公告)日：2010-11-11
申请号：PCT/US2010/032575
申请日：2010-04-27
申请人： UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE , SHI, Xiao-Wen , LIU, Yi , PAYNE, Gregory, F. , YANG, Xiaohua
发明人： SHI, Xiao-Wen , LIU, Yi , PAYNE, Gregory, F. , YANG, Xiaohua
IPC分类号： C12N11/04 , C08B37/04 , C08B37/12
CPC分类号： C12N11/04 , B01J2219/00576 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00725 , B01J2219/00743 , C09D105/04 , G01N33/5438
摘要： Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.
摘要翻译：描述了通过电沉积聚合物电解形成的水凝胶的产生方法。水凝胶可以含有一个或多个电解地址或电可寻址到水凝胶内的位置的细胞群，并且其中细胞群的细胞被水凝胶包埋并且能够在水凝胶内膨胀并且可以从水凝胶中释放。还提供了用于细胞群体的膜内扩增的电可寻址多糖共混物，允许细胞的探查和免疫复合物的形成。还提供了在膜内生物处理方法如基于细胞的生物传感，基于蛋白质的生物传感和细胞信号传导研究中使用含有电解或电可寻址细胞群的水凝胶的方法。

4. 发明申请

WO2009137211A3 FIBROUS ASSEMBLIES FOR ANTIBODY PRESENTATION, AND MULTIPLEXED ANTIGENIC ANALYSIS USING SAME 审中-公开
公开(公告)号：WO2009137211A3
公开(公告)日：2009-11-12
申请号：PCT/US2009/039799
申请日：2009-04-07
申请人： UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE OFF. OF RESEARCH ADMIN./ TECH. DEV. , SHI, Xiao-wen , WU, Hsuan-chen , PAYNE, Gregory, F. , BENTLEY, William, E.
发明人： SHI, Xiao-wen , WU, Hsuan-chen , PAYNE, Gregory, F. , BENTLEY, William, E.
IPC分类号： G01N33/53 , G01N33/68 , G01N33/544
摘要： Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g. , histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g. , in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.

5. 发明申请

WO2010040047A2 CHITOSAN-COATED WIRES FOR BIOSENSING 审中-公开
标题翻译：用于生化的CHITOSAN涂层线
公开(公告)号：WO2010040047A2
公开(公告)日：2010-04-08
申请号：PCT/US2009/059372
申请日：2009-10-02
申请人： UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE OFF. OF RESEARCH ADMIN./TECH.DEV. , LIU, Yi , SHI, Xiao-Wen , PAYNE, Gregory, F. , MEYER, W., Lee
发明人： LIU, Yi , SHI, Xiao-Wen , PAYNE, Gregory, F. , MEYER, W., Lee
IPC分类号： G01N33/50
CPC分类号： G01N33/54393
摘要： A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electrodepositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.
摘要翻译：一种在导电性基材上形成包含蛋白质的生物电子装置的方法，通过在基材上施加阴极电压将氨基多糖壳聚糖电沉积在基底上，在其上形成氨基多糖壳聚糖膜，在其上施加阳极电压至基材的NaCl以活化氨基多糖壳聚糖膜，使其与蛋白质反应。该方法还可选地包括使氨基多糖膜在其活化后与蛋白质反应，使得蛋白质组装在基底上并与基底结合，从而形成生物电子器件。蛋白质可以包括单个或多个蛋白质物质，并且包括生物传感蛋白质。另外的方法包括存在于样品中或在生物识别事件期间产生的电化学活性化合物的生物传感以及在这些方法中有用的装置。所得到的装置可用作手持装置，纺织品，服装等中的传感器。

6. 发明申请

WO2009137211A2 FIBROUS ASSEMBLIES FOR ANTIBODY PRESENTATION, AND MULTIPLEXED ANTIGENIC ANALYSIS USING SAME 审中-公开
标题翻译：用于抗体呈递的纤维组件，以及使用相同的多重抗原分析
公开(公告)号：WO2009137211A2
公开(公告)日：2009-11-12
申请号：PCT/US2009039799
申请日：2009-04-07
申请人： UNIV MARYLAND BIOTECH INST , SHI XIAO-WEN , WU HSUAN-CHEN , PAYNE GREGORY F , BENTLEY WILLIAM E
发明人： SHI XIAO-WEN , WU HSUAN-CHEN , PAYNE GREGORY F , BENTLEY WILLIAM E
IPC分类号： G01N33/53 , G01N33/544 , G01N33/68
CPC分类号： A61K9/5169 , B01J19/0046 , B01J2219/00515 , B01J2219/00524 , B01J2219/00527 , B01J2219/00657 , B01J2219/00659 , B01J2219/00666 , B01J2219/00725 , C08L5/08 , G01N33/544
摘要： Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.
摘要翻译：生物功能化的纤维，包括纤维平台和组氨酸标记的蛋白质以及任选的抗体。壳聚糖是一种可用作纤维平台的纤维。纤维平台可用镍处理或可直接连接至组氨酸标记的蛋白，例如组氨酸标记的链球菌IgG结合蛋白，蛋白G，蛋白G3T，GFP或RFP。所得到的生物功能化纤维可以通过多种生物加工方法组装成蛋白质纤维组件。纤维组件，例如以机织织物形式，可用于（i）抗原捕获; （ii）免疫分析，和/或（iii）多重分析。在一种制造中，纤维组件的每根纤维呈现特定的抗体，并且纤维的混合和匹配（例如通过编织来自各种抗体呈递纤维的织物）允许多种抗原被同时捕获用于多路分析。

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