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    • 6. 发明申请
    • SESQUITERPENE SYNTHASES FROM PATCHOULI
    • 来自PATCHOULI的SESQUITERPENE SYNTHASES
    • WO2005056803A1
    • 2005-06-23
    • PCT/IB2003/006459
    • 2003-12-09
    • FIRMENICH SASCHALK, Michel
    • SCHALK, Michel
    • C12N15/60
    • C12N9/88
    • The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, 7-curcumene and other germacrane-type sesquiterpenes.
    • 本发明涉及广藿香植物(Pogostemon cablin)的倍半萜合酶及其生产和使用方法。 在一个实施方案中,本发明提供了包含编码至少一种倍半萜合酶的如本文所述的核苷酸序列的核酸。 在另一个实施方案中,本发明还提供倍半萜合酶和制备和使用这些酶的方法。 例如,本发明的倍半萜烯合酶可用于将法呢基焦磷酸酯转化成各种倍半萜烯,包括patchoulol,7-姜黄素和其他锗类倍半萜烯。
    • 8. 发明申请
    • METHOD FOR PRODUCING SCLAREOL
    • 生产SCLAREOL的方法
    • WO2009095366A1
    • 2009-08-06
    • PCT/EP2009/050816
    • 2009-01-26
    • FIRMENICH SASCHALK, Michel
    • SCHALK, Michel
    • C12P7/02C12N9/14C12N15/52
    • C12P7/02C12N9/16C12P7/18
    • The present invention provides a method of producing sclareol,said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol,a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
    • 本发明提供了生产香紫苏醇的方法,所述方法包括将具有香紫苏醇合成酶活性的特定多肽与二硫代二磷酸酯(LPP)接触。 特别地,所述方法可以在体外或体内进行以产生香紫苏醇,香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 编码本发明多肽的编码本发明多肽的核酸,含有所述核酸的表达载体以及转化成携带相同核酸的非人生物体或细胞也是本发明的一部分。