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1. 发明申请

US20060249558A1 Automated micro-volume assay system 有权
标题翻译：自动微量测定系统
公开(公告)号：US20060249558A1
公开(公告)日：2006-11-09
申请号：US11401699
申请日：2006-04-10
申请人： David Roach , Tom Yang , Roger O'Neill , Robert Loder , Peter Vander Horn
发明人： David Roach , Tom Yang , Roger O'Neill , Robert Loder , Peter Vander Horn
IPC分类号： A47J36/02
CPC分类号： F16L41/00 , G01N35/04 , G01N35/1065 , G01N2035/1034 , G01N2035/1039 , G01N2035/1051 , G01N2035/1062 , Y10T137/0318 , Y10T137/85938 , Y10T436/11 , Y10T436/119163 , Y10T436/2575
摘要： An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation and immobilization station where reactions inside the capillaries take place and a detector station where photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run. Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format.
摘要翻译：描述了用于放置用于毛细管内部的材料测定的材料的站的自动测定系统和用于操纵毛细管的自动化夹具。该系统包括分离和固定站，其中毛细管内部发生反应，并且检测到来自毛细血管反应的光摄取的检测器站。来自毛细血管的光摄取可以显示为线图或类似于熟悉的Western凝胶印迹的假凝胶图像的列。自动化控制系统具有用户界面，通过该界面，操作员可以选择运行协议并定义要在协议运行中使用的样本和试剂的位置。在设置之后，控制系统将使自动化系统执行协议，然后以选定的显示格式显示结果。

2. 发明授权

US6146511A Electrophoretic nucleic acid purification method 失效
标题翻译：电泳核酸纯化方法
公开(公告)号：US6146511A
公开(公告)日：2000-11-14
申请号：US016531
申请日：1998-01-30
申请人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
发明人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
IPC分类号： B01D57/02 , B03C5/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12N15/10 , G01N27/447 , G01N27/26
CPC分类号： C12N15/101 , G01N27/447
摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.
摘要翻译：公开了一种用于纯化核酸样品的电泳方法。该方法通常包括以下步骤：（1）提供包含所需核酸和一种或多种污染物的核酸样品，（2）提供具有载体孔的电泳基质和其中形成的回收孔，（3）（4）进行第一次电泳，其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳，其包括将核酸样品电泳第二次，有效将所需核酸转运到电泳基质内并进入回收井。根据该方法，第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的，从而产生纯化的核酸。在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。还公开了采用交流电场的制备电泳方法。

3. 发明申请

US20060292649A1 Methods and apparatus for reference lab diagnostics 审中-公开
标题翻译：参考实验室诊断的方法和装置
公开(公告)号：US20060292649A1
公开(公告)日：2006-12-28
申请号：US11431343
申请日：2006-05-09
申请人： Linda Cahill , Roger O'Neill
发明人： Linda Cahill , Roger O'Neill
IPC分类号： G01N33/558
CPC分类号： G01N33/54366 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44726 , G01N27/44795 , G01N33/558 , G01N33/582 , G01N33/6896 , G01N2800/2828 , Y02A50/57
摘要： Automated protein assay apparatus and methods suitable for use in a reference lab are described. A screening test for a pathogen or disease condition is performed at the reference lab. If the results of the screening test are positive, a definitive assay is performed. One or more analytes are resolved in a fluid path such as that of a capillary by isoelectric focusing. The resolved analytes are immobilized in the capillary by photoimmobilization. A typical analyte is a protein of a biological sample of a subject. Detection agents such as antibodies are flowed through the capillary which bind to or interact with the analytes, forming antibody-protein complexes. A chemiluminescent substrate is flowed through the capillary and detected with a photon detector. The detected locations of the analytes are recorded and the results sent to the referring physician. The assay is preferably performed by a computer-controlled, automated system exhibiting speed, precision and repeatability which are highly desirable for a reference lab diagnostic test.
摘要翻译：描述适用于参考实验室的自动蛋白质测定装置和方法。在参考实验室进行病原体或疾病状况的筛选试验。如果筛选试验的结果为阳性，则进行确定的测定。一个或多个分析物通过等电聚焦在诸如毛细管的流体路径中解析。通过光固化将解析的分析物固定在毛细管中。典型的分析物是受试者的生物样品的蛋白质。诸如抗体的检测剂流过毛细管，其结合或与分析物相互作用，形成抗体 - 蛋白质复合物。化学发光底物流过毛细管并用光子检测器检测。记录检测到的分析物的位置，并将结果发送给转诊医师。该测定优选通过显示速度，精度和重复性的计算机控制的自动化系统进行，这对于参考实验室诊断测试是非常需要的。

4. 发明申请

US20080009078A1 Methods and devices for analyte detection 有权
标题翻译：用于分析物检测的方法和装置
公开(公告)号：US20080009078A1
公开(公告)日：2008-01-10
申请号：US11725769
申请日：2007-03-19
申请人： Roger O'Neill , Marc Glazer , Tom Yang , Daniel Suich , Karl Voss
发明人： Roger O'Neill , Marc Glazer , Tom Yang , Daniel Suich , Karl Voss
IPC分类号： G01N33/566 , B05D3/00 , G01N33/00
CPC分类号： G01N33/54366 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44726 , G01N27/44795 , G01N33/582
摘要： Methods and apparatus are provided to resolve analytes within a fluid path using isoelectric focusing, gel electrophoresis, or other separation means. Materials within the fluid path that are compatible with these separation means are used to attach resolved analytes to the wall of the fluid path. Attachment results from a triggerable event such as photoactivation, thermal activation, or chemical activation. In accordance with a further aspect of the present invention, the material in the capillary may also be disrupted, by either the triggerable event or a subsequent event such as melting or photocleavage. Thus, an open lumen or porous structure may be created within the fluid path, allowing unbound analyte materials to be washed from the fluid path, and detection agents to be washed into the fluid path. The separation-compatible materials may be polymerizable monomers, gels, entangled polymers or other materials.
摘要翻译：提供了使用等电聚焦，凝胶电泳或其他分离方法来解决流体路径内的分析物的方法和装置。使用与这些分离装置相容的流体路径内的材料将分解的分析物附着到流体路径的壁上。附件来自可触发事件，如光活化，热激活或化学活化。根据本发明的另一方面，毛细管中的材料也可以通过可触发事件或随后的事件（例如熔化或光切割）而被破坏。因此，可以在流体路径内产生开放的内腔或多孔结构，允许从流体路径洗涤未结合的分析物质，以及要洗涤到流体路径中的检测剂。分离相容材料可以是可聚合单体，凝胶，缠结聚合物或其它材料。

5. 发明申请

US20060292558A1 Methods and apparatus for protein assay diagnostics 审中-公开
标题翻译：蛋白质测定诊断方法和装置
公开(公告)号：US20060292558A1
公开(公告)日：2006-12-28
申请号：US11431272
申请日：2006-05-09
申请人： Roger O'Neill
发明人： Roger O'Neill
IPC分类号： C12Q1/70 , G01N33/554 , G01N33/569
CPC分类号： G01N33/54366 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44726 , G01N27/44795 , G01N33/582 , Y02A50/57
摘要： Automated protein assay apparatus and methods for measuring antibodies against an analyte are described. A standard mixture of one or more analytes is loaded into a capillary, the analytes are resolved by isoelectric focusing and immobilized in the capillary. Serum from a human or non-human subject under analysis is flowed through the capillary and antibodies specific for the immobilized analytes bind to the analytes. A secondary antibody including a detectable marker is introduced, binding to the immobilized antibody-analyte complexes. The locations of the antibody-analyte complexes are detected by means of the detectable markers, revealing the presence of analyte-specific antibodies in the serum.
摘要翻译：描述了用于测量针对分析物的抗体的自动蛋白质测定装置和方法。将一种或多种分析物的标准混合物加载到毛细管中，通过等电聚焦来分析分析物并固定在毛细管中。来自分析的人或非人受试者的血清流过毛细管，并且对固定化分析物特异的抗体与分析物结合。引入包含可检测标记的二抗，与固定的抗体 - 分析物复合物结合。通过可检测的标记检测抗体 - 分析物复合物的位置，揭示血清中分析物特异性抗体的存在。

6. 发明申请

US20060257917A1 FLUORESCENT DYES, ENERGY TRANSFER COUPLES AND METHODS 审中-公开
标题翻译：荧光染料，能量转移接头和方法
公开(公告)号：US20060257917A1
公开(公告)日：2006-11-16
申请号：US11461770
申请日：2006-08-01
申请人： Roger O'Neill , Peter Fisher
发明人： Roger O'Neill , Peter Fisher
IPC分类号： C12Q1/68 , C07H21/04 , C07D405/14 , C07D403/14
CPC分类号： C09B11/08 , C09B11/06 , C09B11/24 , C09B11/28 , C09B23/083 , G01N33/542
摘要： Fluorescent dyes, fluorescence energy transfer dye couples, multi-color dye sets, can be employed in art-recognized assays and certain novel methods, such as in proximity assays.
摘要翻译：荧光染料，荧光能量转移染料对，多色染料组，可以用于本领域公认的测定和某些新颖的方法，例如邻近测定。

7. 发明授权

US06638760B1 Method and apparatus for flow-through hybridization 有权
标题翻译：流通杂交的方法和装置
公开(公告)号：US06638760B1
公开(公告)日：2003-10-28
申请号：US09204865
申请日：1998-12-03
申请人： Jer-Kang Chen , Claudia Chiesa , George A. Fry , Vergine C. Furniss , Stephen M. Lambert , Roger O'Neill , Majid Mehrpouyan
发明人： Jer-Kang Chen , Claudia Chiesa , George A. Fry , Vergine C. Furniss , Stephen M. Lambert , Roger O'Neill , Majid Mehrpouyan
IPC分类号： C12M134
CPC分类号： C12Q1/6834 , C12Q1/6837 , C12Q2565/629 , C12Q2565/507 , C12Q2525/197
摘要： The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.
摘要翻译：本发明提供了用于核酸的有效，快速和特异性捕获以及最佳回收的底物和装置，以及其使用方法。底物本质上是多孔的，并且具有能够固定在其上的靶核酸杂交的捕获多核苷酸。当含有或怀疑含有靶核酸的样品通过多孔底物流动时，靶核酸被快速捕获。捕获后，靶核酸可以有效地回收以备后续使用。

8. 发明授权

US06827830B1 Electrophoretic nucleic acid purification method 有权
标题翻译：电泳核酸纯化方法
公开(公告)号：US06827830B1
公开(公告)日：2004-12-07
申请号：US09666905
申请日：2000-09-20
申请人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
发明人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
IPC分类号： G01N27447
CPC分类号： C12N15/101 , G01N27/447
摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.
摘要翻译：公开了一种用于纯化核酸样品的电泳方法。根据该方法，电泳有效地相对于核酸样品中所需核酸的浓度显着降低污染物的浓度，从而产生纯化的核酸。在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。

9. 发明授权

US07091348B2 Fluorescent dyes, energy transfer couples and methods 有权
标题翻译：荧光染料，能量转移对和方法
公开(公告)号：US07091348B2
公开(公告)日：2006-08-15
申请号：US10612297
申请日：2003-07-01
申请人： Roger O'Neill , Peter V. Fisher
发明人： Roger O'Neill , Peter V. Fisher
IPC分类号： C07D209/06 , C07D215/04 , C07D487/02
CPC分类号： C09B11/08 , C09B11/06 , C09B11/24 , C09B11/28 , C09B23/083 , G01N33/542
摘要： Fluorescent dyes, fluorescence energy transfer dye couples, multi-color dye sets, can be employed in art-recognized assays and certain novel methods, such as in proximity assays.
摘要翻译：荧光染料，荧光能量转移染料对，多色染料组，可以用于本领域公认的测定和某些新颖的方法，例如邻近测定。

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