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    • 3. 发明授权
    • Gel cassette for electrophoresis
    • 凝胶盒用于电泳
    • US6027628A
    • 2000-02-22
    • US28180
    • 1998-02-23
    • Hidetaka YamamuraRichard T. L. Chan
    • Hidetaka YamamuraRichard T. L. Chan
    • G01N27/447G01N27/26G01K27/447
    • G01N27/44704G01N27/44721
    • A gel cassette for electrophoresis includes opposed plastic plates holding a gel therebetween. An upper buffer tank can be adhered to one of the plates by double backed tape that is sandwiched between the tank and plate, thereby avoiding the use of a rubber sealing gasket between the tank and plate. As a result, the distance between the tank and the plate is kept constant. A buffer solution in the tank communicates with the gel. The cassette can then be placed in a lower buffer tank and an electric field applied to the buffer solutions to effect electrophoresis of DNA fragments that have been placed in the gel. In an alternate embodiment, a window is formed in one of the plastic plates to allow laser light to pass into the cassette and excite a fluorescent tag in the DNA. The fluorescence then passes back through the window for detection by a sensor, to automatically determine the DNA sequence of the sample in the gel in accordance with auto DNA sequencing principles. The window reduces and indeed eliminates absorption, interference, and/or reflection of the relatively weak fluorescent signal emitted by the DNA tag.
    • 用于电泳的凝胶盒包括在其间保持凝胶的相对的塑料板。 上缓冲罐可以通过夹在罐和板之间的双背带粘合到其中一个板上,从而避免在罐和板之间使用橡胶密封垫圈。 结果,罐和板之间的距离保持恒定。 罐中的缓冲溶液与凝胶相通。 然后可以将盒放置在下缓冲罐中,并将电场施加到缓冲溶液以实现已经放置在凝胶中的DNA片段的电泳。 在替代实施例中,窗口形成在一个塑料板中,以允许激光进入盒并激发DNA中的荧光标签。 荧光然后通过窗口返回通过传感器检测,以根据自动DNA测序原理自动确定凝胶中样品的DNA序列。 窗口减少并且确实消除了由DNA标签发射的相对较弱的荧光信号的吸收,干扰和/或反射。
    • 5. 发明授权
    • Running tank assembly for electrophoresis
    • 电泳运行槽组件
    • US06402915B1
    • 2002-06-11
    • US09354292
    • 1999-07-15
    • Robert G. BeckerRichard T. L. ChanMin Kar Moi
    • Robert G. BeckerRichard T. L. ChanMin Kar Moi
    • G01N2726
    • G01N27/44704
    • An electrophoresis running tank assembly includes a running tank and a power supply holder that is integral to the running tank. A lid is configured for covering the tank, with two activation arms depending downwardly from the lid and with peripheral vertical skirts of the lid overlapping the walls of the tank. Two switches are held by a switch assembly in the power supply holder and are accessible through a space between the power supply holder and a pin wall that supports two connector pins. When the lid is engaged with the tank, the activation arms extend into the space to abut and thereby close the switches, thereby electrically connecting the switches to the electrode. A power supply can be engaged with the pins to energize the electrodes. Owing to the cooperation between the skirts and the walls of the tank, the lid cannot be tilted relative to the tank to expose the electrodes, but instead must deliberately be lifted up and away from the tank (thus opening the switches and deenergizing the electrodes) to expose the electrodes.
    • 电泳运行箱组件包括运行箱和与运行箱一体的电源座。 盖子构造成用于覆盖箱体,其中两个活动臂从盖子向下悬挂,并且盖子的外围垂直裙边与罐壁重叠。 两个开关由电源保持器中的开关组件保持,并且可通过电源保持器和支撑两个连接器引脚的插脚壁之间的空间接近。 当盖与罐接合时,致动臂延伸到空间中以邻接并因此闭合开关,从而将开关电连接到电极。 电源可以与引脚接合以激励电极。 由于裙子和水箱壁之间的配合,盖子不能相对于水箱倾斜以露出电极,而是必须有意地被提起并远离水箱(从而打开开关并使电极断电) 以暴露电极。