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1. 发明申请

US20100330674A1 CELL CULTURE SUPPORT AND ASSOCIATED METHOD FOR CELL GROWTH AND RELEASE 有权
标题翻译：细胞培养支持和细胞生长和释放的相关方法
公开(公告)号：US20100330674A1
公开(公告)日：2010-12-30
申请号：US12493331
申请日：2009-06-29
申请人： Slawomir Rubinsztajn , Prameela Susarla , Anton Beletskii , Reginald Donovan Smith , Brian Polizzotti
发明人： Slawomir Rubinsztajn , Prameela Susarla , Anton Beletskii , Reginald Donovan Smith , Brian Polizzotti
IPC分类号： C12N5/00
CPC分类号： C12N5/0075 , C08F8/12 , C08F293/005 , C12M23/20 , C12M33/00 , C12N5/0068 , C12N2533/30 , C12N2539/10
摘要： A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.
摘要翻译：一种细胞培养支持物，其包含底物和固定在底物上的双重刺激响应性嵌段共聚物，其中所述双重刺激响应性嵌段共聚物是热反应性和pH响应性的。一种培养细胞的方法，所述细胞包含具有固定在底物上的双重刺激响应共聚物的细胞培养支持物，其中所述双重刺激响应共聚物是热反应性和pH响应性的; 并在细胞培养支持下培养细胞。通过降低温度，细胞从细胞培养支持物释放。

2. 发明申请

US20100151438A1 METHODS AND KITS FOR ENHANCING SEDIMENTATION AND RECOVERY OF CELLS IN A SAMPLE 审中-公开
标题翻译：用于增强样品中的细胞的测定和恢复的方法和工具
公开(公告)号：US20100151438A1
公开(公告)日：2010-06-17
申请号：US12333926
申请日：2008-12-12
申请人： Liming Yu , Hongyi Zhou , Nichole Lee Wood , Anup Sood , Reginald Donovan Smith , Brian David Polizzotti
发明人： Liming Yu , Hongyi Zhou , Nichole Lee Wood , Anup Sood , Reginald Donovan Smith , Brian David Polizzotti
IPC分类号： A01N1/02
CPC分类号： G01N33/491
摘要： The methods and kits provide sedimentation-enhancing agents that significantly increase the efficiency of cell-sedimentation. They also increase the efficiency of blood cell separation methods and thereby increase the recovery of total nucleated cells.
摘要翻译：方法和试剂盒提供沉淀增强剂，显着提高细胞沉淀的效率。它们还提高了血细胞分离方法的效率，从而增加了总有核细胞的回收率。

3. 发明授权

US08399252B2 Methods and kits for cell release 有权
公开(公告)号：US08399252B2
公开(公告)日：2013-03-19
申请号：US12570032
申请日：2009-09-30
申请人： Slawomir Rubinsztain , Prameela Susarla , Reginald Donovan Smith , Evelina Roxana Loghin
发明人： Slawomir Rubinsztain , Prameela Susarla , Reginald Donovan Smith , Evelina Roxana Loghin
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12M23/20 , C12M33/00 , C12N2533/32 , C12N2539/10
摘要： Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.

4. 发明授权

US09453196B2 Cell carrier, methods of making and use 有权
标题翻译：细胞载体，制备和使用方法
公开(公告)号：US09453196B2
公开(公告)日：2016-09-27
申请号：US13287611
申请日：2011-11-02
申请人： Reginald Donovan Smith , Scott Michael Miller , Yosang Yoon , Slawomir Rubinsztajn , Prameela Susarla
发明人： Reginald Donovan Smith , Scott Michael Miller , Yosang Yoon , Slawomir Rubinsztajn , Prameela Susarla
IPC分类号： C12N5/071 , C12N5/00
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/30 , C12N2535/10
摘要： A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 μm, and width in a range from about 20 to 200 μm; seeding the cells on the carriers; and growing the cells on the carrier.
摘要翻译：提供培养贴壁细胞的方法，其中所述方法包括提供用于生长所述细胞的载体，包含一个或多个表面; 并且在一个或多个表面上存在一个或多个消除特征/凹陷，其中载体具有至少约0.2mm，宽度至少约0.2mm的长度，以及在约0.012mm至0.5的范围内的高度毫米并且其中每个所述浮雕特征/压痕的表面高度在约2至200μm的范围内，宽度在约20至200μm的范围内; 将细胞接种在载体上; 并在载体上生长细胞。

5. 发明申请

US20100133181A1 SYSTEMS AND METHODS FOR PROCESSING COMPLEX BIOLOGICAL MATERIALS 有权
标题翻译：用于处理复杂生物材料的系统和方法
公开(公告)号：US20100133181A1
公开(公告)日：2010-06-03
申请号：US12325672
申请日：2008-12-01
申请人： Nichole Lea Wood , Reginald Donovan Smith , Hongyi Zhou , Liming Yu , Brian Polizzotti , Peter Miller
发明人： Nichole Lea Wood , Reginald Donovan Smith , Hongyi Zhou , Liming Yu , Brian Polizzotti , Peter Miller
IPC分类号： B01D61/24 , B01D61/58 , B01D61/30 , B01D65/02 , B01D61/32
CPC分类号： G01N33/491 , A61M1/029 , B01D61/28 , B01D61/30 , B01D63/02 , B01D2313/18 , B01D2313/50 , C12N5/0647 , C12N5/0665 , G01N1/4077 , G01N2001/4083 , G01N2001/4088
摘要： Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.
摘要翻译：用于处理在容器中含有生物材料的生物材料的方法和系统; 向容器中的材料添加聚集剂，并使材料分离成两个或更多不同的子材料; 从容器中提取一种或多种副材料; 将剩余在容器中的一种或多种副材料自动输送到过滤装置; 并将得到的目标滞留物收集到目标滞留容器中。

6. 发明申请

US20080308422A1 Protein Sample Preparation 审中-公开
标题翻译：蛋白质样品制备
公开(公告)号：US20080308422A1
公开(公告)日：2008-12-18
申请号：US11762564
申请日：2007-06-13
申请人： Reginald Donovan Smith , Erin Jean Finehout
发明人： Reginald Donovan Smith , Erin Jean Finehout
IPC分类号： G01N27/26 , G01N1/38 , G01N37/00
CPC分类号： G01N27/44704 , G01N1/38 , Y10T436/108331
摘要： In one aspect, the invention provides methods for protein sample preparation for electrophoretic separation. The method comprises a step of providing a protein sample in solution, adding a chaotrope to the protein sample, adding a surfactant to the protein sample, wherein the final concentration of the surfactant in the solution is less than critical micelle concentration of the surfactant. In another aspect, the invention also provides methods of electrophoretic separation of protein samples that includes the protein sample preparation method as described herein. In yet another aspect, the invention provides a loading buffer solution composition for protein sample preparation that includes a surfactant in solution at a final concentration that is less than critical micelle concentration of the surfactant, and; a chaotrope.
摘要翻译：一方面，本发明提供了用于电泳分离的蛋白质样品制备方法。该方法包括在溶液中提供蛋白质样品，向蛋白质样品中加入离液剂，向蛋白质样品中加入表面活性剂的步骤，其中溶液中表面活性剂的最终浓度小于表面活性剂的临界胶束浓度。另一方面，本发明还提供了包括如本文所述的蛋白质样品制备方法的蛋白质样品的电泳分离方法。另一方面，本发明提供了用于蛋白质样品制备的负载缓冲溶液组合物，其包含溶液中的表面活性剂，其终浓度小于表面活性剂的临界胶束浓度，一种离液剂

7. 发明授权

US09469839B2 Cell culture support and associated method for cell growth and release 有权
标题翻译：细胞培养支持和相关的细胞生长和释放方法
公开(公告)号：US09469839B2
公开(公告)日：2016-10-18
申请号：US12493331
申请日：2009-06-29
申请人： Slawomir Rubinsztajn , Prameela Susarla , Anton Beletskii , Reginald Donovan Smith , Brian Polizzotti
发明人： Slawomir Rubinsztajn , Prameela Susarla , Anton Beletskii , Reginald Donovan Smith , Brian Polizzotti
IPC分类号： C12M3/00 , C12N5/00 , C08F293/00 , C12M1/26 , C12M1/00
CPC分类号： C12N5/0075 , C08F8/12 , C08F293/005 , C12M23/20 , C12M33/00 , C12N5/0068 , C12N2533/30 , C12N2539/10
摘要： A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.
摘要翻译：一种细胞培养支持物，其包含底物和固定在底物上的双重刺激响应性嵌段共聚物，其中所述双重刺激响应性嵌段共聚物是热反应性和pH响应性的。一种培养细胞的方法，所述细胞包含具有固定在底物上的双重刺激响应共聚物的细胞培养支持物，其中所述双重刺激响应共聚物是热反应性和pH响应性的; 并在细胞培养支持下培养细胞。通过降低温度，细胞从细胞培养支持物释放。

8. 发明申请

US20120156777A1 CELL CARRIER, ASSOCIATED METHODS FOR MAKING CELL CARRIER AND CULTURING CELLS USING THE SAME 审中-公开
标题翻译：细胞载体，用于制备细胞载体和培养细胞的相关方法
公开(公告)号：US20120156777A1
公开(公告)日：2012-06-21
申请号：US12970735
申请日：2010-12-16
申请人： Arvind Rangarajan , Prameela Susarla , Scott Michael Miller , Reginald Donovan Smith , Slawomir Rubinsztajn , Anton Beletskii
发明人： Arvind Rangarajan , Prameela Susarla , Scott Michael Miller , Reginald Donovan Smith , Slawomir Rubinsztajn , Anton Beletskii
IPC分类号： C12N5/071 , B05D3/12 , C12N5/02 , C12M1/00 , C12M1/24 , C12M1/22 , C23C16/513 , B32B38/06
CPC分类号： C12N5/0068 , C12M25/14 , C12N2533/30 , C12N2535/10 , Y10T156/1039
摘要： A carrier for growing adherent cells is provided, wherein the carrier comprises one or more outer surfaces; and one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. The carrier may comprise a single indentation or ‘cup’ like structure, or may comprise a plurality of indentations. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
摘要翻译：提供了用于生长贴壁细胞的载体，其中载体包括一个或多个外表面; 以及在一个或多个外表面上的一个或多个结构化凹陷，其中载体具有至少约0.2mm，宽度至少约0.2mm的长度，以及约0.05mm至1.2mm范围内的高度，的结构化缺口具有在约0.1mm至0.5mm的范围内的长轴，在约0.1mm至0.5mm的范围内的短轴和在约0.025mm至约0.5mm的范围内的深度。载体可以包括单个凹陷或“杯状”结构，或者可以包括多个凹陷。还提供了制造载体和使用相同载体培养基质细胞的方法。

9. 发明申请

US20120156773A1 CELL CARRIER, METHODS OF MAKING AND USE 有权
标题翻译：细胞载体，制备和使用方法
公开(公告)号：US20120156773A1
公开(公告)日：2012-06-21
申请号：US13287611
申请日：2011-11-02
申请人： Reginald Donovan Smith , Scott Michael Miller , Yosang Yoon , Slawomir Rubinsztajn , Prameela Susarla
发明人： Reginald Donovan Smith , Scott Michael Miller , Yosang Yoon , Slawomir Rubinsztajn , Prameela Susarla
IPC分类号： C12N5/071 , C12N5/077
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/30 , C12N2535/10
摘要： A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 μm, and width in a range from about 20 to 200 μm; seeding the cells on the carriers; and growing the cells on the carrier.
摘要翻译：提供培养贴壁细胞的方法，其中所述方法包括提供用于生长所述细胞的载体，包含一个或多个表面; 并且在一个或多个表面上存在一个或多个消除特征/凹陷，其中载体具有至少约0.2mm，宽度至少约0.2mm的长度，以及在约0.012mm至0.5的范围内的高度毫米并且其中每个所述浮雕特征/压痕的表面高度在约2至200μm的范围内，宽度在约20至200μm的范围内; 将细胞接种在载体上; 并在载体上生长细胞。

10. 发明申请

US20110070596A1 AGENTS AND METHODS FOR ANALYZING PROTEIN INTERACTIONS 审中-公开
标题翻译：用于分析蛋白质相互作用的试剂和方法
公开(公告)号：US20110070596A1
公开(公告)日：2011-03-24
申请号：US12705828
申请日：2010-02-15
申请人： Nichole Lea Wood , Mohan Mark Amaratunga , Hans Grade , Faisal Ahmed Syud , Anup Sood , Reginald Donovan Smith , Ayse Betul Dinc , Amy Casey Williams , Anthony John Murray , Gregory Daryll Goddard
发明人： Nichole Lea Wood , Mohan Mark Amaratunga , Hans Grade , Faisal Ahmed Syud , Anup Sood , Reginald Donovan Smith , Ayse Betul Dinc , Amy Casey Williams , Anthony John Murray , Gregory Daryll Goddard
IPC分类号： G01N33/68 , C07D403/12 , G01N33/53
CPC分类号： G01N33/6845 , G01N33/6848
摘要： Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library.
摘要翻译：提供了使用同位素标记的对称双功能交联剂和质谱定性和定量分析蛋白质复合物或蛋白质复合物的试剂和方法。靶向部分，细胞渗透性部分或亲和部分可附加到双功能交联剂上。同位素标记的对称双功能交联剂可用于试剂盒或文库。

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