专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2013033049A8 BIOCOATED PIEZOELECTRIC BIOSENSOR PLATFORM FOR POINT-OF-CARE DIAGNOSTIC USE 审中-公开
公开(公告)号：WO2013033049A8
公开(公告)日：2013-03-07
申请号：PCT/US2012/052583
申请日：2012-08-27
申请人： AVIANA MOLECULAR TECHNOLOGIES, LLC , LAURY-KLEINTOP, Lisa , OU, Hsu-Cheng , RUTNER, Herman
发明人： LAURY-KLEINTOP, Lisa , OU, Hsu-Cheng , RUTNER, Herman
IPC分类号： H01L41/00
摘要： Biosensor components (chips )are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezoelectrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table monolayers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biotinylated antibody for capture and detection of analytes in biologic fluid samples. Although the processes described herein can be performed on any type of piezoelectric material in any number of configurations, some embodiments are directed to a biosensor with the foregoing biocoating onto a particular acoustic plate mode biosensor and where the interdigitated transducers (IDTs) are present on the opposite side of the crystal's biocoated film.

2. 发明申请

WO2013033049A1 BIOCOATED PIEZOELECTRIC BIOSENSOR PLATFORM FOR POINT-OF-CARE DIAGNOSTIC USE 审中-公开
标题翻译：点对点诊断用生物聚合压电生物传感器平台
公开(公告)号：WO2013033049A1
公开(公告)日：2013-03-07
申请号：PCT/US2012052583
申请日：2012-08-27
申请人： AVIANA MOLECULAR TECHNOLOGIES LLC , LAURY-KLEINTOP LISA , OU HSU-CHENG , RUTNER HERMAN
发明人： LAURY-KLEINTOP LISA , OU HSU-CHENG , RUTNER HERMAN
IPC分类号： H01L41/00
CPC分类号： G01N33/54306 , B05D3/142 , G01D5/14 , G01N29/02 , G01N29/022 , G01N29/22 , G01N29/34 , G01N29/36 , G01N33/54386 , G01N33/56927 , G01N33/56983 , G01N2291/0255 , G01N2291/0256 , G01N2291/0427 , H01L41/113 , H01L41/253
摘要： Biosensor components (chips )are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezoelectrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table monolayers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biotinylated antibody for capture and detection of analytes in biologic fluid samples. Although the processes described herein can be performed on any type of piezoelectric material in any number of configurations, some embodiments are directed to a biosensor with the foregoing biocoating onto a particular acoustic plate mode biosensor and where the interdigitated transducers (IDTs) are present on the opposite side of the crystal's biocoated film.
摘要翻译：基于直接生物涂层方法描述生物传感器组件（芯片），其导致锚定物质如抗生物素蛋白具有特异性结合伴侣或寡核苷酸或多核苷酸的其他蛋白质的顽固且稳定，非共价（被认为是化学吸收）到任何压电活性晶体表面。所产生的平台技术可以针对具有生物测定中特定应用的各种生物传感器开发。锚定物质的表单层形成反应层，准备结合捕获试剂例如生物素化抗体以捕获和检测生物流体样品中的分析物。虽然本文所述的方法可以以任何数量的配置的任何类型的压电材料进行，但是一些实施例涉及具有上述生物涂层的生物传感器到特定的声板模式生物传感器上，并且其中叉指式换能器（IDT）存在于水晶生物涂层膜的对面。

3. 发明申请

WO2005049168A2 METHOD AND APPARATUS FOR PRE-ENRICHMENT AND RECOVERY OF CELLS FROM DENSIFIED WHOLE BLOOD 审中-公开
标题翻译：从浓缩全血中预先富集和回收细胞的方法和设备
公开(公告)号：WO2005049168A2
公开(公告)日：2005-06-02
申请号：PCT/US2004/038609
申请日：2004-11-17
申请人： IMMUNIVEST CORPORATION , GROSS, Steven , RUTNER, Herman
发明人： GROSS, Steven , RUTNER, Herman
IPC分类号： B01D
CPC分类号： A01N1/02 , A01N1/0263
摘要： The present invention describes reagents, methods and apparatus for pre-processing of blood samples to obtain blood fractions containing substantially all target cells in reduced specimen volumes that are suitable for subsequent further enrichment. Sample processor systems are described for enriching cell populations having densities of less than approximately 1.09 g/ml from about 30 ml whole blood. Whole blood is combined with a cell compatible dense reagent to raise the density to about 1.09 g/ml. Centrifugation causes the target cells and most of the WBC in the buffy coat layer to accumulate at the top under a low-density buffer overlay. The reduced sample volumes are suitable for subsequent immunomagnetic or other enrichment methods prior to detection and enumeration. These processor systems, in conjunction with other enrichment procedures, facilitate detection of rare target cells, such as circulating tumor cells (CTC) that are useful markers in cancer diagnosis and monitoring of therapeutic response.
摘要翻译：本发明描述了用于对血液样品进行预处理以获得含有基本上全部靶细胞的血液馏分的试剂，方法和设备，所述血液馏分具有适合于随后进一步富集的减少的样本体积。描述了用于从约30ml全血富集密度小于约1.09g / ml的细胞群的样品处理器系统。将全血与细胞相容致密试剂组合以将密度提高至约1.09g / ml。离心使血块层中的靶细胞和大部分WBC在低密度缓冲覆盖层下积聚在顶部。在检测和计数之前，减少的样品体积适合随后的免疫磁性或其他富集方法。这些处理器系统与其他富集程序相结合，有助于检测罕见的靶细胞，如循环肿瘤细胞（CTC），这些细胞是癌症诊断和治疗反应监测的有用标记物。

4. 发明申请

WO2006054991A1 MAGNETIC ENRICHMENT OF CIRCULATING CELLS, FRAGMENTS AND DEBRIS FOR ENABLING HTS PROTEOMICS AND GENOMICS IN DISEASE DETECTION 审中-公开
标题翻译：循环细胞的磁性增殖，用于启动HTS预防和疾病感染的基因组的片段和修饰
公开(公告)号：WO2006054991A1
公开(公告)日：2006-05-26
申请号：PCT/US2004/038608
申请日：2004-11-17
申请人： IMMUNIVEST CORPORATION , DOYLE, Gerald, V. , O'HARA, Shawn, Mark , RUTNER, Herman
发明人： DOYLE, Gerald, V. , O'HARA, Shawn, Mark , RUTNER, Herman
IPC分类号： G01N33/574 , G01N33/567 , G01N33/566 , B03C1/00 , C07K16/00
CPC分类号： G01N33/574 , G01N33/5091
摘要： The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry, CellSpotter TM fluorescent microscopy imaging andmass spectrometric profiling. In addition to analyzing and enumerating morphologicaly classified cells by imaging, the presence of damaged cells and derived debris in magnetically enriched specimens has been shown to be an important “smoking gun” indicator of demised tumor cells. Described herein are improved methods to screen, diagnose and monitor disease based on intact circulating rare cells and associated fragments or debris. The present invention incorporates proteomic and genomic profiling of cellular debris in cancer cell analysis, either complementary to or independent of image cytometry. The technology provides diagnosis and prognostic tools in diseases such as cancer that incorporate not only detection and quantitation of intact circulating cells but also of cellular fragments and debris that bear generic cytoskeletal and/or tumor-specific markers. The presence of such markers reflects immune function in early disease and/or therapeutic effectiveness in later stages.
摘要翻译：本发明描述的方法和试剂用于分析循环肿瘤细胞，簇，碎片和碎片。使用许多平台进行分析，包括流式细胞术，CellSpotter TM荧光显微镜成像和质谱分析。除了通过成像分析和列举形态分类的细胞之外，磁性富集标本中损伤的细胞和衍生碎片的存在已经被证明是消除肿瘤细胞的重要的“吸烟枪”指标。本文描述了基于完整的循环稀有细胞和相关片段或碎片筛选，诊断和监测疾病的改进方法。本发明将细胞碎片的蛋白质组学和基因组分析结合在癌细胞分析中，与图像细胞计数的补充或独立。该技术在诸如癌症的疾病中提供了诊断和预后工具，其不仅包括完整的循环细胞的检测和定量，而且还包含具有通用细胞骨架和/或肿瘤特异性标志物的细胞碎片和碎片。这种标志物的存在反映早期疾病中的免疫功能和/或后期阶段的治疗有效性。

5. 发明申请

WO2005028663A3 OPERATOR INDEPENDENT PROGRAMMABLE SAMPLE PREPARATION AND ANALYSIS SYSTEM 审中-公开
公开(公告)号：WO2005028663A3
公开(公告)日：2005-03-31
申请号：PCT/US2004/030429
申请日：2004-09-17
申请人： IMMUNIVEST CORPORATION , BENDELE, Teresa , HARBART, Thomas , HOWARD, Dave , KAGAN, Michael , KEENE, Douglas , LAPEUS, Dave , MAYES, Jared , PAYNTER, Douglas , PROHASKA, Jerry , RUTNER, Herman
发明人： BENDELE, Teresa , HARBART, Thomas , HOWARD, Dave , KAGAN, Michael , KEENE, Douglas , LAPEUS, Dave , MAYES, Jared , PAYNTER, Douglas , PROHASKA, Jerry , RUTNER, Herman
IPC分类号： G01N21/00
摘要： The present invention provides a protocol and apparatus for enriching circulating tumor cells and other rare cells from blood, including debris and other components, from samples with high precision and at high throughput rates. This invention discloses an improved processing system from previously described semi-automated sample processing. The system further reduces operator intervention and hands-on time from prior systems. While this system has general utility in processing diverse materials, the system is configured for sample processing of biological specimens to provide an enriched fraction suitable for detection, enumeration and identification of target cells by appropriate analytical methodologies. The presence and quantities of such target cells in a sample specimen can utilized for screening and detection in disease such as cancer, assessing early stage pre-metastatic cancer, monitoring for disease remission in response to therapy and selection of more effective dose regimens or alternative therapies in case of relapse.

6. 发明申请

WO2005028663A2 OPERATOR INDEPENDENT PROGRAMMABLE SAMPLE PREPARATION AND ANALYSIS SYSTEM 审中-公开
标题翻译：操作者独立的可编程样品制备和分析系统
公开(公告)号：WO2005028663A2
公开(公告)日：2005-03-31
申请号：PCT/US2004030429
申请日：2004-09-17
申请人： IMMUNIVEST CORP , BENDELE TERESA , HARBART THOMAS , HOWARD DAVE , KAGAN MICHAEL , KEENE DOUGLAS , LAPEUS DAVE , MAYES JARED , PAYNTER DOUGLAS , PROHASKA JERRY , RUTNER HERMAN
发明人： BENDELE TERESA , HARBART THOMAS , HOWARD DAVE , KAGAN MICHAEL , KEENE DOUGLAS , LAPEUS DAVE , MAYES JARED , PAYNTER DOUGLAS , PROHASKA JERRY , RUTNER HERMAN
IPC分类号： C12Q20060101 , G01N1/00 , G01N1/40 , G01N21/00 , G01N31/00 , G01N33/00 , G01N33/53 , G01N35/00 , G06T7/00 , H01L21/265 , C12Q
CPC分类号： G01N35/0098 , G01N1/40 , G06T7/70 , G06T2207/30004
摘要： The present invention provides a protocol and apparatus for enriching circulating tumor cells and other rare cells from blood, including debris and other components, from samples with high precision and at high throughput rates. This invention discloses an improved processing system from previously described semi-automated sample processing. The system further reduces operator intervention and hands-on time from prior systems. While this system has general utility in processing diverse materials, the system is configured for sample processing of biological specimens to provide an enriched fraction suitable for detection, enumeration and identification of target cells by appropriate analytical methodologies. The presence and quantities of such target cells in a sample specimen can utilized for screening and detection in disease such as cancer, assessing early stage pre-metastatic cancer, monitoring for disease remission in response to therapy and selection of more effective dose regimens or alternative therapies in case of relapse.
摘要翻译：本发明提供了一种用于从高精度和高生产率的样品中富集来自血液的循环肿瘤细胞和其它稀有细胞（包括碎片和其他成分）的方案和装置。本发明公开了一种来自先前描述的半自动化样品处理的改进的处理系统。该系统进一步减少了现有系统的操作员干预和实际操作时间。虽然该系统在处理不同材料方面具有一般效用，但是该系统被配置用于生物样品的样品处理，以通过适当的分析方法提供适合于靶细胞的检测，计数和鉴定的富集级分。样品样本中的这种靶细胞的存在和数量可以用于疾病如癌症中的筛选和检测，评估早期转移前癌症，响应于治疗监测疾病缓解和选择更有效的剂量方案或替代疗法在复发的情况下。

7. 发明申请

WO2010138871A1 INTEGRATED MICROCHIP SURFACE ACOUSTIC WAVE SENSOR SYSTEM FOR DETECTION OF INFECTIOUS AGENTS 审中-公开
标题翻译：一体化微波表面声波传感器系统，用于检测感染性细胞
公开(公告)号：WO2010138871A1
公开(公告)日：2010-12-02
申请号：PCT/US2010/036662
申请日：2010-05-28
申请人： AVIANA MOLECULAR TECHNOLOGIES, LLC , RAGAVAN, Vanaja, Vijaya , ROY, Avijit , RUTNER, Herman
发明人： RAGAVAN, Vanaja, Vijaya , ROY, Avijit , RUTNER, Herman
IPC分类号： G01N29/02 , G01N29/22 , G01N30/00 , G01N33/00
CPC分类号： G01N29/022 , G01N29/222 , G01N29/226 , G01N33/54373 , G01N33/569 , G01N2291/0255 , G01N2291/0256 , G01N2291/0423 , G01N2291/106 , Y02A50/53
摘要： An integrated multiplexed acoustic wave biosensor chip system with enhanced sensitivity has been developed. The biosensor system incorporates one or more microfluidic channels, coated with target-specific binding films enabling rapid and early detection of viral, bacterial or parasitic targets such as Dengue virus and sexually transmitted diseases in specimens from potentially infected patients. The biosensors are used in portable analytical systems that are suitable for real-time point of care (POC) clinical diagnosis in cost sensitive and/or resource limited settings. The highly sensitive biosensors utilize thinned single crystal piezoelectric substrates that propagate layer guided shear horizontal acoustic plate mode (LG-SH-APM) waves in sensing regions bearing immobilized binders that provide simultaneous and direct detection of mass changes due to multiple bound target pathogens or molecules.
摘要翻译：已经开发了具有增强的灵敏度的集成多声道声波生物传感器芯片系统。生物传感器系统包含一个或多个微流体通道，涂覆有目标特异性结合膜，能够在潜在感染患者的标本中快速和早期检测病毒，细菌或寄生虫目标，如登革热病毒和性传播疾病。生物传感器用于便携式分析系统，适用于成本敏感和/或资源有限的实时临床诊断中的实时护理点（POC）。高灵敏度的生物传感器利用薄膜单晶压电基片，传播层导向剪切水平声板模式（LG-SH-APM）波在感测具有固定粘合剂的区域，提供同时和直接检测多重结合靶病原体或分子的质量变化。

8. 发明申请

WO2005049168A3 METHOD AND APPARATUS FOR PRE-ENRICHMENT AND RECOVERY OF CELLS FROM DENSIFIED WHOLE BLOOD 审中-公开
标题翻译：用于预处理和恢复来自透明血液的细胞的方法和装置
公开(公告)号：WO2005049168A3
公开(公告)日：2005-09-09
申请号：PCT/US2004038609
申请日：2004-11-17
申请人： IMMUNIVEST CORP , GROSS STEVEN , RUTNER HERMAN
发明人： GROSS STEVEN , RUTNER HERMAN
IPC分类号： A01N1/02 , B01D20060101
CPC分类号： A01N1/02 , A01N1/0263
摘要： The present invention describes reagents, methods and apparatus for pre-processing of blood samples to obtain blood fractions containing substantially all target cells in reduced specimen volumes that are suitable for subsequent further enrichment. Sample processor systems are described for enriching cell populations having densities of less than approximately 1.09 g/ml from about 30 ml whole blood. Whole blood is combined with a cell compatible dense reagent to raise the density to about 1.09 g/ml. Centrifugation causes the target cells and most of the WBC in the buffy coat layer to accumulate at the top under a low-density buffer overlay. The reduced sample volumes are suitable for subsequent immunomagnetic or other enrichment methods prior to detection and enumeration. These processor systems, in conjunction with other enrichment procedures, facilitate detection of rare target cells, such as circulating tumor cells (CTC) that are useful markers in cancer diagnosis and monitoring of therapeutic response.
摘要翻译：本发明描述了用于预处理血液样品以获得含有基本上所有靶细胞的血液成分的试剂，方法和装置，其中所述靶细胞在减少的样品体积中适合于随后的进一步富集。描述了用于从约30ml全血中富集密度小于约1.09g / ml的细胞群的样品处理器系统。将全血与细胞相容的致密试剂结合，将密度提高至约1.09μg/ ml。离心导致目标细胞和血沉棕黄层中的大部分WBC在低密度缓冲层覆盖下在顶部积聚。在检测和计数之前，减少的样品体积适用于随后的免疫磁性或其它富集方法。这些处理器系统与其他富集程序相结合，便于检测罕见的靶细胞，例如循环肿瘤细胞（CTC），它们是癌症诊断和治疗反应监测中有用的标志物。

9. 发明申请

WO03018757A3 STABILIZATION OF CELLS AND BIOLOGICAL SPECIMENS FOR ANALYSIS 审中-公开
标题翻译：细胞稳定和生物样本分析
公开(公告)号：WO03018757A3
公开(公告)日：2003-09-12
申请号：PCT/US0226867
申请日：2002-08-23
申请人： IMMUNIVEST CORP , RAO GALLA CHANDRA , HERMANN MELISSA , RUTNER HERMAN , TERSTAPPEN LEON
发明人： RAO GALLA CHANDRA , HERMANN MELISSA , RUTNER HERMAN , TERSTAPPEN LEON
IPC分类号： G01N33/48 , A01N1/00 , A01N1/02 , C12Q1/02 , G01N33/49 , G01N33/543 , G01N33/569 , G01N33/574
CPC分类号： A01N1/02 , A01N1/00 , A01N1/0226 , G01N33/54333 , G01N33/574
摘要： Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable n protocols requiring rare cells enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.
摘要翻译：公开了用于稳定血液标本中稀有细胞，保持血液标本质量以及用作细胞固定剂的组合物和方法，其使靶细胞（例如，循环肿瘤细胞）的损失最小化，并从靶细胞形成碎片和聚集体，非靶细胞和血浆成分，从而允许更准确地分析和分类循环肿瘤细胞（CTC），最终导致癌症患者的肿瘤负担。标本的稳定是特别需要的，需要来自癌症患者的血液标本稀有细胞富集的方案。已经观察到这些样品暴露于例如在正常加工，混合，摇动，由于运送血液引起的延迟所遇到的潜在压力条件下，不仅减少了CTC的数量，而且在血样中产生碎片和聚集体被发现干扰靶细胞的准确计数（如果存在）。鉴别体内CTC分解和体外样品降解是必需的稳定剂。

10. 发明申请

WO2003019141A3 ANALYSIS OF CIRCULATING TUMOR CELLS, FRAGMENTS, AND DEBRIS 审中-公开
公开(公告)号：WO2003019141A3
公开(公告)日：2003-03-06
申请号：PCT/US2002/026861
申请日：2002-08-23
申请人： IMMUNIVEST CORPORATION , RAO, Galla, Chandra , LARSON, Christopher , REPOLLET, Madeline , RUTNER, Herman , TERSTAPPEN, Leon , O'HARA, Shawn, Mark , GROSS, Steven
发明人： RAO, Galla, Chandra , LARSON, Christopher , REPOLLET, Madeline , RUTNER, Herman , TERSTAPPEN, Leon , O'HARA, Shawn, Mark , GROSS, Steven
IPC分类号： C07K1/00
摘要： The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro . Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式