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1. 发明申请

WO2012045568A1 METHOD FOR DETERMINING A BIOLOGICAL PATHWAY ACTIVITY 审中-公开
标题翻译：确定生物路径活动的方法
公开(公告)号：WO2012045568A1
公开(公告)日：2012-04-12
申请号：PCT/EP2011/066223
申请日：2011-09-19
申请人： bioMérieux , HOSPICES CIVILS DE LYON , MIOSSEC, Pierre , MOUGIN, Bruno , PAYE, Malick , REYNIER, Frederic
发明人： MIOSSEC, Pierre , MOUGIN, Bruno , PAYE, Malick , REYNIER, Frederic
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q2600/106 , C12Q2600/158
摘要： Method for determining a level of activity of a biological pathway from a biological sample of a patient suffering from a pathology, said method comprising the steps of: measuring the level of expression of at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is inactive, said at least three genes being associated to said biological pathway, for establishing a negative reference, and measuring level of expression of said at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is active, for establishing a positive reference, and measuring the level of expression of said at least three genes of said patient in the sample, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the negative reference and determining a value C- which corresponds to a correlation level between the level of expression of said at least three genes of said negative reference and the level of expression of said at least three genes of said patient, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the positive reference and determining a value C+ which corresponds to a correlation level between the level of expression of said at least three genes of said positive reference and the level of expression of said at least three genes of said patient, and establishing a ratio C+/C- which gives a correlation score, wherein if the correlation score is lower to 1 that is indicative of the non activity of the biological pathway, and if the correlation score is upper or equal to 1 that is indicative of the activity of the biological pathway.
摘要翻译：用于确定来自病理学患者的生物样品的生物学途径的活性水平的方法，所述方法包括以下步骤：测量来自一组个体，对照个体或患者的至少三种基因的表达水平所述至少三个基因与所述生物学途径相关联，用于建立负参考，以及测量来自一组个体，对照个体或患者的所述至少三种基因的表达水平，用于生物学途径是活性的，用于建立阳性参照，以及测量所述患者所述至少三个基因在样品中的表达水平，并比较所述患者样品中所述至少三个基因的表达水平所述至少三个基因的所述负参考的表达水平，并确定对应于相关水平的值C- 在所述负参考的所述至少三个基因的表达水平与所述患者的所述至少三个基因的表达水平之间，并将所述患者样品中所述至少三个基因的表达水平与所述阳性参考文献的所述至少三个基因的表达水平，并确定对应于所述阳性参考文献的所述至少三个基因的表达水平与所述至少三个基因的表达水平之间的相关水平的值C + 并建立给出相关分数的比率C + / C-，其中如果所述相关得分低于表示所述生物学途径的非活性的1，并且如果所述相关得分高于或等于1 这表明生物学途径的活性。

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