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    • 9. 发明申请
    • NUCLEOTIDE PROBES AND METHOD FOR DETERMINING HLA DQB1 TYPING
    • 核糖核酸探针和确定HLA DQB1型的方法
    • WO1997046700A1
    • 1997-12-11
    • PCT/FR1997000980
    • 1997-06-03
    • BIO MERIEUXMOUGIN, Bruno
    • BIO MERIEUX
    • C12Q01/68
    • C12Q1/6881C12Q2600/156
    • Nucleotide probe selected in particular among the following: TG CGT TAT GTG ACC AGA , GT G CGT CTT GTG ACC AGA , GT CTT GTA ACC AGA CAC AT, CG T CTT GTA ACC AGA TAC AT, CG T CTT GTG AGC AGA AGC AT, CG ACC GAG CTC GTG CGG CG T G, G T AC CGG GCA GTG AC G C, G A CG CCG CTG GGI CCG CCT G , G A CG CCG CTG GGG CCG CCT G , G G AG GGI ACC CIG GCI GA G T, G G AG GGG ACC CGG GCG GA G T, TC G GTG GAC ACC GTA TGC AG A C, GG ACG GAG CGC GTG CG, C ATC TAT AAC CGA GA , and their complementary sequences, being understood that the said probes have at least the underlined sequence, and can further contain one or two bases selected, respecting the sequence continuity, among the non-underlined bases. These probes are useful for determining a person's HLA DQ beta genotype. They have in particular the advantage of being useable at a single hybridization temperature, in particular 37 DEG C.
    • 选择以下特定的核苷酸探针:TG CGT TAT GTG ACC AGA,GTG CGT CTT GTG ACC AGA,GTCTT GTA ACC AGA CAC AT,CGT CTT GTA ACC AGA TAC AT,CGT CTT GTG AGC AGA AGC AT,CG ACC GAG CTC GTG CGG CG TG ,GT ACGG GCA GTG AC GC,GA CG CCG CTG GGI CCG CCT G,GA CG CCG CTG GGG CCG CCT G,GG < AG AG GGI ACC CIG GCI GA GT,GGGGGG ACC CGG GCG GA GT,TCG GTG GAC ACC GTA TGC AG AC,GG ACG GAG CGC GTG CG,C ATC TAT AAC CGA GA及其互补序列,应理解为所述探针至少具有下划线序列,并且还可含有一个或两个 在不带下划线的基础之间选择了基于序列连续性的基因。 这些探针可用于确定一个人的HLA DQβ基因型。 它们特别具有在单一杂交温度,特别是37℃下可用的优点。
    • 10. 发明申请
    • METHOD FOR DETERMINING A BIOLOGICAL PATHWAY ACTIVITY
    • 确定生物路径活动的方法
    • WO2012045568A1
    • 2012-04-12
    • PCT/EP2011/066223
    • 2011-09-19
    • bioMérieuxHOSPICES CIVILS DE LYONMIOSSEC, PierreMOUGIN, BrunoPAYE, MalickREYNIER, Frederic
    • MIOSSEC, PierreMOUGIN, BrunoPAYE, MalickREYNIER, Frederic
    • C12Q1/68
    • C12Q1/6883C12Q2600/106C12Q2600/158
    • Method for determining a level of activity of a biological pathway from a biological sample of a patient suffering from a pathology, said method comprising the steps of: measuring the level of expression of at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is inactive, said at least three genes being associated to said biological pathway, for establishing a negative reference, and measuring level of expression of said at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is active, for establishing a positive reference, and measuring the level of expression of said at least three genes of said patient in the sample, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the negative reference and determining a value C- which corresponds to a correlation level between the level of expression of said at least three genes of said negative reference and the level of expression of said at least three genes of said patient, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the positive reference and determining a value C+ which corresponds to a correlation level between the level of expression of said at least three genes of said positive reference and the level of expression of said at least three genes of said patient, and establishing a ratio C+/C- which gives a correlation score, wherein if the correlation score is lower to 1 that is indicative of the non activity of the biological pathway, and if the correlation score is upper or equal to 1 that is indicative of the activity of the biological pathway.
    • 用于确定来自病理学患者的生物样品的生物学途径的活性水平的方法,所述方法包括以下步骤:测量来自一组个体,对照个体或患者的至少三种基因的表达水平 所述至少三个基因与所述生物学途径相关联,用于建立负参考,以及测量来自一组个体,对照个体或患者的所述至少三种基因的表达水平,用于 生物学途径是活性的,用于建立阳性参照,以及测量所述患者所述至少三个基因在样品中的表达水平,并比较所述患者样品中所述至少三个基因的表达水平 所述至少三个基因的所述负参考的表达水平,并确定对应于相关水平的值C- 在所述负参考的所述至少三个基因的表达水平与所述患者的所述至少三个基因的表达水平之间,并将所述患者样品中所述至少三个基因的表达水平与 所述阳性参考文献的所述至少三个基因的表达水平,并确定对应于所述阳性参考文献的所述至少三个基因的表达水平与所述至少三个基因的表达水平之间的相关水平的值C + 并建立给出相关分数的比率C + / C-,其中如果所述相关得分低于表示所述生物学途径的非活性的1,并且如果所述相关得分高于或等于1 这表明生物学途径的活性。