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1. 发明授权

US06775621B1 Degree of hybridization detection method 失效
标题翻译：杂交检测方法
公开(公告)号：US06775621B1
公开(公告)日：2004-08-10
申请号：US09459712
申请日：1999-12-13
申请人： Noriko Yurino , Kenji Yamamoto , Toshiaki Ito , Toshimasa Watanabe
发明人： Noriko Yurino , Kenji Yamamoto , Toshiaki Ito , Toshimasa Watanabe
IPC分类号： G01N3348
CPC分类号： C12Q1/6837 , C12Q2545/114 , C12Q2545/101 , C12Q2563/107 , C12Q2565/102
摘要： The present invention provides a hybridization detection method capable of quantitatively determining the degree of the hybridization between a sample biopolymer and a probe biopolymer. In the method, the amount of a fluorescently labeled probe immobilized on a substrate as a spot is quantitatively determined by determining the fluorescence emitted from a fluorescent material labeling the probe, and the amount of a fluorescently labeled sample hybridized to the probe is quantitatively determined by determining the fluorescence emitted from the fluorescent material labeling the sample. The difference between the amount of the probe and the amount of the sample is normalized with the amount of the probe. Based on the normalized value, the amount of the sample hybridized to the probe can be determined relative to the amount of the probe spotted on the substrate.
摘要翻译：本发明提供能够定量测定样品生物聚合物和探针生物聚合物之间的杂交程度的杂交检测方法。在该方法中，通过测定从标记探针的荧光物质发出的荧光来定量测定固定在基板上的荧光标记探针作为斑点的量，并且通过用探针杂交的荧光标记的样品的量定量地测定确定从标记样品的荧光材料发出的荧光。探针的量与样品量之间的差异用探针的量进行归一化。基于归一化值，可以相对于在基板上点样的探针的量来确定与探针杂交的样品的量。

2. 发明授权

US06755952B1 Device for electrophoresis 失效
标题翻译：电泳装置
公开(公告)号：US06755952B1
公开(公告)日：2004-06-29
申请号：US09787128
申请日：2001-03-13
申请人： Kenji Yamamoto , Noriko Yurino
发明人： Kenji Yamamoto , Noriko Yurino
IPC分类号： G01N27453
CPC分类号： G01N27/44791 , G01N27/44721
摘要： The present invention allows detection in short time while maintaining the level of the resolving power as high as when a capillary is utilized. The entire surface of an electrophoresis board 10, which is provided with a two-dimensional tubular electrophoresis pathway, is irradiated with excitation light so as to pick up fluorescence or luminescence from a sample with a two-dimensional sensor. By reading the entire electrophoresis pathway, the separated state of the sample may be confirmed during the electrophoresis. As a result, the electrophoresis may be ended when the sample is sufficiently separated and a molecular weight thereof may be determined based on the migration distance.
摘要翻译：本发明允许在短时间内进行检测，同时保持分辨能力的水平高达使用毛细管时的高度。设置有二维管状电泳通路的电泳板10的整个表面用激发以便从具有二维传感器的样品中吸收荧光或发光。通过读取整个电泳途径，可以在电泳期间确认样品的分离状态。结果，当样品充分分离并且可以基于迁移距离确定分子量时，可以结束电泳。

3. 发明授权

US6077670A Fluorescent material labeled-probe and method for detecting hybridization 失效
标题翻译：荧光材料标记探针和杂交检测方法
公开(公告)号：US6077670A
公开(公告)日：2000-06-20
申请号：US39278
申请日：1998-03-12
申请人： Kenji Yamamoto , Hisanori Nasu , Hitoshi Fujimiya , Noriko Yurino
发明人： Kenji Yamamoto , Hisanori Nasu , Hitoshi Fujimiya , Noriko Yurino
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N37/00
CPC分类号： C12Q1/6818
摘要： A method is disclosed which is capable of increasing a S/N ratio without subjecting a sample to any treatment to thereby detect hybridization with high sensitivity. A fragmentarily divided probe is used which is prepared by dividing a single probe 12 into a plurality of fragmentary probes 12a, 12b, and labeling the fragmentary probes with different fluorescent materials 14a, 14b, respectively. When the probe is hybridized with a sample 11, the fragmentary probes 12a,12b reconstitute the single probe. An intermittent light beam is used as excitation light 19. The fluorescent material 14a is excited therewith, and fluorescence 18 emitted from the fluorescent material 14b through multistep excitation is detected while irradiation with the excitation light is intermitted.
摘要翻译：公开了能够提高S / N比而不对样品进行任何处理从而以高灵敏度检测杂交的方法。使用通过将单个探针12分成多个部分探针12a，12b并分别用不同的荧光材料14a，14b标记碎片探针来制备的片断分离的探针。当探针与样品11杂交时，片段探针12a，12b重构单个探针。使用间歇光束作为激发光19.荧光材料14a被激发，并且通过多脉冲激发从荧光材料14b发射的荧光18被检测，同时激发光的照射被中断。

4. 发明授权

US6071702A Probe-bearing element and method for producing the same 失效
标题翻译：承载元件及其制造方法
公开(公告)号：US6071702A
公开(公告)日：2000-06-06
申请号：US364752
申请日：1999-07-30
申请人： Kenji Yamamoto , Noriko Yurino , Hisanori Nasu
发明人： Kenji Yamamoto , Noriko Yurino , Hisanori Nasu
IPC分类号： C12Q1/00 , B01J19/00 , B01L3/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N37/00 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6834 , B01J19/0046 , B01L3/5085 , B01J2219/00317 , B01J2219/00319 , B01J2219/00527 , B01J2219/00531 , B01J2219/00536 , B01J2219/00538 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00664 , B01J2219/00722 , B01L2300/0893 , C40B40/06 , C40B60/14
摘要： A probe-bearing element according to the present invention includes a sheet-like body having a plurality of through holes which each bears a biopolymer probe. The sheet-like body may have a thickness of 100 .mu.m to 2 mm, and may be made of glass, acrylic resin, metal or plastic. Preferably, the size of the through hole is about 10-100 .mu.m in diameter considering the number of samples relative to the size of the element, amounts of probes required for hybridization and the detection sensitivity. The plurality of through holes are preferably arranged in concentric circles or in a spiral.
摘要翻译：根据本发明的探针承载元件包括具有多个通孔的片状体，其各自承载生物聚合物探针。片状体的厚度可以为100μm〜2mm，并且可以由玻璃，丙烯酸树脂，金属或塑料制成。考虑到相对于元件尺寸的样品数量，杂交所需的探针量和检测灵敏度，优选地，通孔的直径大约为10-100μm。多个通孔优选地以同心圆或螺旋形布置。

5. 发明授权

US06563584B1 Method and device for fluorescence measurement 失效
标题翻译：用于荧光测量的方法和装置
公开(公告)号：US06563584B1
公开(公告)日：2003-05-13
申请号：US09743681
申请日：2001-03-15
申请人： Noriko Yurino , Kenji Yamamoto , Kentaro Shishido
发明人： Noriko Yurino , Kenji Yamamoto , Kentaro Shishido
IPC分类号： G01N2125
CPC分类号： G01N21/6452 , B01J2219/00536 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C40B40/06 , G01N27/44721 , G01N33/487 , G01N33/582 , G01N35/00069 , G01N2201/0833
摘要： The invention uses a continuous-wave laser as an excitation light source to achieve highly-accurate detection with a simple structure. A sample 11a is irradiated with excitation light from an excitation light source 30, and then transported to an optical axis of a fluorescence detector 50, thereby being detected of its fluorescence intensity.
摘要翻译：本发明使用连续波激光器作为激发光源，以简单的结构实现高精度的检测。用来自激发光源30的激发光照射样品11a，然后将其输送到荧光检测器50的光轴，从而检测其荧光强度。

6. 发明授权

US06127125A Sample application method and device 失效
标题翻译：示例应用方法和设备
公开(公告)号：US06127125A
公开(公告)日：2000-10-03
申请号：US264463
申请日：1999-03-08
申请人： Noriko Yurino , Kenji Yamamoto , Hisanori Nasu
发明人： Noriko Yurino , Kenji Yamamoto , Hisanori Nasu
IPC分类号： G01N1/00 , B01L99/00 , C40B40/06 , C40B60/14 , G01N1/28 , G01N35/00 , C12Q1/68
CPC分类号： G01N1/2813 , B01L3/0293 , B01J2219/00364 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , B01L2300/0636 , B01L2300/0803 , B01L2300/18 , B01L2400/0409 , C40B40/06 , C40B60/14 , G01N2035/00158 , Y10S435/81
摘要： A sample application method including the steps of: rotating a biochip including a plurality of regions bound with various probes; and dropping a sample onto generally a center of rotation of the rotating biochip so as to apply the sample to the plurality of regions.
摘要翻译：一种样品施加方法，包括以下步骤：旋转包括与各种探针结合的多个区域的生物芯片; 并将样品落在旋转的生物芯片的大致旋转中心上，以将样品施加到多个区域。

7. 发明授权

US5991030A Apparatus for reading a luminescence pattern of a sample 失效
标题翻译：用于读取样品的发光图案的装置
公开(公告)号：US5991030A
公开(公告)日：1999-11-23
申请号：US66875
申请日：1998-04-28
申请人： Kenji Yamamoto , Hisanori Nasu , Toshimasa Watanabe , Yuuji Tsukamoto , Tateo Kondou , Takehiko Nishida , Hitoshi Fujimiya , Noriko Yurino
发明人： Kenji Yamamoto , Hisanori Nasu , Toshimasa Watanabe , Yuuji Tsukamoto , Tateo Kondou , Takehiko Nishida , Hitoshi Fujimiya , Noriko Yurino
IPC分类号： G01N27/447 , G01R9/02 , F21V9/16
CPC分类号： G01N27/44721
摘要： The apparatus for reading a luminescence pattern is equipped with a stage on which the sample to be read is mounted. A photoreceptor disk is installed in the lower part of the stage and scans and condenses light from the luminescent pattern of the sample at positions determined by the rotation of a rotary plate. A transport mechanism causes the stage and the photoreceptor disk to undergo relative motion. An optical guide part guides the light from the luminescent pattern to a photoelectric converter. The optical guide part may include mirrors or one or more optical fibers. The photoelectric converter converts the light into an electrical signal. A controller controls how scanning is performed by the photoreceptor disk and transport mechanism and generates a read scanning position signal. A data processor performs data processing by converting the electrical signal into a digital signal and obtains a scanning position signal from the controller. Additionally, a laser light source may be used to shine laser light onto an electrophoresis gel of the sample.
摘要翻译：用于读取发光图案的装置装备有待安装样品的阶段。感光盘安装在舞台的下部，并且通过旋转板旋转确定的位置扫描并冷凝来自样品的发光图案的光。传送机构使舞台和感光盘发生相对运动。光导部分将光从发光图案引导到光电转换器。光导部分可以包括反射镜或一个或多个光纤。光电转换器将光转换成电信号。控制器控制感光盘和传送机构的扫描方式，并生成读取扫描位置信号。数据处理器通过将电信号转换为数字信号并从控制器获得扫描位置信号来执行数据处理。此外，可以使用激光源将激光照射到样品的电泳凝胶上。

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