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1. 发明申请

WO2003012142A1 DETECTION OF NUCLEIC ACIDS BY REAL-TIME PCR USING CHIMERIC RNA-DNA PRIMERS 审中-公开
标题翻译：通过使用CHIMERIC RNA-DNA PRIMERS实时PCR检测核酸
公开(公告)号：WO2003012142A1
公开(公告)日：2003-02-13
申请号：PCT/NL2002/000519
申请日：2002-07-31
申请人： NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK , WAGENINGEN UNIVERSITY , SYBESMA, Wilbert, Feike, Henricus , KLEEREBEZEM, Michiel
发明人： SYBESMA, Wilbert, Feike, Henricus , KLEEREBEZEM, Michiel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q2561/113 , C12Q2525/161
摘要： The present invention relates to methods for detection and quantification of target sequences in nucleic acid sample using real-time PCR. The method advantageously employs a chimeric RNA:DNA primer that introduces a unique (universal) detection sequence during a first gene-specific primer-extension reaction. The introduction of this unique detection sequence allows to use a single (universal) fluorescently labelled detection probe for detection and quantification of multiple different target sequence specific amplification products in real-time polymerase chain reactions. The method may be applied for detection and quantification of both DNA and RNA target sequences and is preferably used for quantification of specific mRNA's. By introducing a primer ligation step the method is adapted for genome wide transcriptome analysis. The method can be adapted for differential gene expression analysis in a single PCR reaction, allowing simultaneous and comparative analysis of specific gene-expression levels in samples obtained from cells grown under different conditions.
摘要翻译：本发明涉及使用实时PCR检测和定量核酸样品中靶序列的方法。该方法有利地采用在第一基因特异性引物延伸反应期间引入独特（通用）检测序列的嵌合RNA：DNA引物。引入这种独特的检测序列允许使用单一（通用）荧光标记的检测探针，用于在实时聚合酶链反应中检测和定量多种不同的靶序列特异性扩增产物。该方法可用于DNA和RNA靶序列的检测和定量，并且优选用于定量特异性mRNA。通过引入引物连接步骤，该方法适用于全基因组转录组学分析。该方法可以在单个PCR反应中适应差异基因表达分析，从而在从不同条件下生长的细胞获得的样品中同时和比较分析特异性基因表达水平。

2. 发明申请

WO2004013332A1 SUBSTRATE CONVERSION 审中-公开
标题翻译：基板转换
公开(公告)号：WO2004013332A1
公开(公告)日：2004-02-12
申请号：PCT/NL2003/000555
申请日：2003-08-01
申请人： NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK , NOORDMAN, Herman, Wouter , HUGENHOLTZ, Jeroen , KLEEREBEZEM, Michiel
发明人： NOORDMAN, Herman, Wouter , HUGENHOLTZ, Jeroen , KLEEREBEZEM, Michiel
IPC分类号： C12N15/53
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0077 , C12P7/18 , C12P7/22 , C12P7/26 , C12P7/42 , C12P7/62 , C12P17/08 , C12R1/225
摘要： The present invention relates to a method for converting a carbon-containing substrate of an NAD(P)H-dependent enzyme. A lactic acid bacterium which expresses the NAD(P)H dependent enzyme is used for the conversion. In this way more than 90 % of a substrate may be converted.
摘要翻译：本发明涉及一种NAD（P）H依赖性酶转化含碳底物的方法。用于表达NAD（P）H依赖性酶的乳酸菌用于转化。以这种方式，可以转化90％以上的底物。

3. 发明申请

WO1991002064A2 MODIFIED PROTEASES AND THEIR USE IN FOODSTUFFS 审中-公开
标题翻译：改良的保护品及其在食品卫生中的应用
公开(公告)号：WO1991002064A2
公开(公告)日：1991-02-21
申请号：PCT/EP1990001302
申请日：1990-08-02
申请人： NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK , VOS, Petrus, Antonius, Josephina , SIEZEN, Roelant, Jan , DE VOS, Willem, Meindert , KOK, Jan , VENEMA, Gerhardus , HAANDRIKMAN, Alfred, Jacques
发明人： NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK
IPC分类号： C12N15/57
CPC分类号： C12N9/52 , C07K2319/00
摘要： The invention relates to DNA fragments containing at least one 'mutant protease gene', which 'mutant protease gene' is understood to mean: a gene made up of sections of parent protease genes of several lactococcal strains, in particular of the parent protease genes of L.lactis subsp. cremoris Wg2 and L.lactis subsp. cremoris SK11, as well as a parent protease gene of a lactococcal strain, in particular the parent protease gene of L.lactis subsp. cremoris Wg2 or the parent protease gene of L.lactis subsp. cremoris SK11, the DNA sequence of which has been altered in a manner such that in the mutant protease for which the gene codes: (a) an amino acid other than that of the 'wild-type' protease is present at at least one site, and/or (b) at at least one site within the first 1350 residues of the amino acid sequence, calculated from the N-terminus, at least one amino acid of the 'wild-type' protease is missing and/or one or more amino acids have been inserted, or (c) at at least two sites separated from each other, one or more amino acids are missing and/or one or more amino acids have been inserted, the cloning vectors containing such a DNA fragment, the transformed host strains containing such a DNA fragment or vector, the mutant proteases obtainable as well as the foodstuffs and flavourings produced with the host strains or mutant proteases meant above.

4. 发明专利

DE2652558C2 未知
公开(公告)号：DE2652558C2
公开(公告)日：1986-08-21
申请号：DE2652558
申请日：1976-11-18
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE, NL
发明人： STADHOUDERS, JACOBUS JOHANNES, BENNEKOM, NL , BERG, GERARD VAN DEN, EDE, NL
IPC分类号： A23C15/06 , A23C15/12 , A23C21/02

5. 发明专利

DE2454238C2 未知
公开(公告)号：DE2454238C2
公开(公告)日：1985-05-23
申请号：DE2454238
申请日：1974-11-15
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE, NL
发明人： STAAL, HERMANNUS JAN, EDE, NL
IPC分类号： A01J25/11 , A01J25/12

6. 发明专利

DE2317452C2 未知
公开(公告)号：DE2317452C2
公开(公告)日：1984-02-09
申请号：DE2317452
申请日：1973-04-06
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE, NL
发明人： DRIESSEN, FRANSISCUS MARTINUS, EDE, NL , STADHOUDERS, JACOBUS JOSEPHUS, BENNEKOM, NL , IRHOVENLAAN, JACOB UBBELS VAN, EDE, NL
IPC分类号： A01J11/00 , A23C9/12 , A23C9/123 , A23C17/00

7. 发明专利

ES2026249T3 未知
公开(公告)号：ES2026249T3
公开(公告)日：1992-04-16
申请号：ES88202744
申请日：1988-11-29
申请人： STICHTING NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK
发明人： KLAVER, FRANCISCUS ADRIANUS MARIA , KINGMA, FEDDE
IPC分类号： A23C9/12 , A23C9/123 , C12M1/12
摘要： Fermented milk products, such as yoghurt and buttermilk, are produced by fermentation of a suitable raw material, such as milk, with different microorganism species, in such a way that microorganisms that are beneficial in the production stage but unfavourably affect the product quality during storage, are kept apart from the fermentation mixture by means of a semipermeable membrane. The process can be carried out in a fed batch system or continuously, preferably with continuous pH control. The products so obtained have improved taste and storage properties.

8. 发明专利

DE2322146C2 未知
公开(公告)号：DE2322146C2
公开(公告)日：1985-06-13
申请号：DE2322146
申请日：1973-05-02
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE, NL
发明人： HUP, GERHARD, EDE, NL , STADHOUDERS, JACOBUS JOSEPHUS, BENNEKOM, NL
IPC分类号： C12N1/20 , A23C19/02

9. 发明专利

DE2932767C2 未知
公开(公告)号：DE2932767C2
公开(公告)日：1984-07-05
申请号：DE2932767
申请日：1979-08-13
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE, NL
发明人： SCHAAP, JOHANNUS E., LUNTEREN, NL , NOOY, PAULUS F.C., BENNEKOM, NL , DE BOER, RUDOLF, WAGENINGEN, NL
IPC分类号： A23C9/142 , A23C19/028
摘要： Semi-hard cheese such as Gouda and Edam is produced by subjecting skimmilk to a first stage ultrafiltration, a second stage diafiltration and a third stage ultrafiltration, adding fat to the resultant concentrate, mixing and cooling the resultant fat-containing concentrate in a first scraped heat exchanger, adding additives for making cheese such as rennet and starter, passing the resultant mixture to a second scraped heat exchanger and placing the mixture in molds to form cheese.

10. 发明专利

DE2256005B2 未知
公开(公告)号：DE2256005B2
公开(公告)日：1980-05-14
申请号：DE2256005
申请日：1972-11-15
申请人： STICHTING BEDRIJVEN VAN HET NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK, EDE (NIEDERLANDE)
发明人： BOER, RUDOLF DE, WAGENINGEN (NIEDERLANDE)
IPC分类号： A21D2/26 , A21D2/36 , A23J1/20 , A23C21/00 , A23C23/00
摘要： A method is provided for the preparation of a milk protein coprecipitate high in baking quality and nutritional value and low in lactose by precipitating the proteins from a mixture of whey, whey concentrates and a low fat milk product through heating and pH control in the presence of CaCl2, recovering the precipitate and washing with an aqueous solution of polyphosphate in an amount that after drying the product has a nitrogen solubility index between 4 and 11.

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