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1. 发明授权

US08969036B2 Process for obtaining antibodies 有权
标题翻译：获得抗体的方法
公开(公告)号：US08969036B2
公开(公告)日：2015-03-03
申请号：US11791107
申请日：2005-11-16
申请人： Mukesh Sehdev , Mariangela Spitali
发明人： Mukesh Sehdev , Mariangela Spitali
IPC分类号： C12P21/06 , A23J1/00 , A61K39/395 , C07K16/00
CPC分类号： C07K16/00 , C07K2317/55
摘要： The present invention relates to the manufacture of recombinant antibodies of therapeutic quality. In particular, the invention relates to methods for increasing the yield of functional antibody from large scale fermentations whereby a cultured host cell sample is subjected to a non-lysing treatment.
摘要翻译：本发明涉及制备治疗质量的重组抗体。特别地，本发明涉及从大规模发酵中提高功能性抗体的产量的方法，由此对培养的宿主细胞样品进行非裂解处理。

2. 再颁专利

USRE44512E1 Methods for producing recombinant proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：USRE44512E1
公开(公告)日：2013-10-01
申请号：US13547892
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/04 , C12N5/00 , C12Q1/68
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

3. 发明申请

US20080044817A1 Methods for Producing Recombinant Proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：US20080044817A1
公开(公告)日：2008-02-21
申请号：US10571367
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/04 , C12N5/00 , C12Q1/68
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

4. 发明申请

US20080003644A1 Process for Obtaining Antibodies 有权
标题翻译：获得抗体的方法
公开(公告)号：US20080003644A1
公开(公告)日：2008-01-03
申请号：US11791107
申请日：2005-11-16
申请人： Mukesh Sehdev , Mariangela Spitali
发明人： Mukesh Sehdev , Mariangela Spitali
IPC分类号： C12P21/08
CPC分类号： C07K16/00 , C07K2317/55
摘要： The present invention relates to the manufacture of recombinant antibodies of therapeutic quality. In particular, the invention relates to methods for increasing the yield of functional antibody from large scale fermentations whereby a cultured host cell sample is subjected to a non-lysing treatment.
摘要翻译：本发明涉及制备治疗质量的重组抗体。特别地，本发明涉及从大规模发酵中提高功能性抗体的产量的方法，由此对培养的宿主细胞样品进行非裂解处理。

5. 发明申请

US20070298462A1 Process for Obtaining Antibodies 审中-公开
标题翻译：获得抗体的方法
公开(公告)号：US20070298462A1
公开(公告)日：2007-12-27
申请号：US11791108
申请日：2005-11-16
申请人： Mukesh Sehdev , Mariangela Spitali
发明人： Mukesh Sehdev , Mariangela Spitali
IPC分类号： C12P21/04
CPC分类号： C07K16/00 , C07K2317/55 , C12P21/02
摘要： The present invention relates to the manufacture of recombinant antibodies of therapeutic quality. In particular, the invention relates to methods for increasig the yield of cuntional antibody from large scale fermentations whereby a cultured host cell sample is subjected to a freeze-thaw treatment step.
摘要翻译：本发明涉及制备治疗质量的重组抗体。特别地，本发明涉及从大规模发酵中提高免疫抗体的产量的方法，由此对培养的宿主细胞样品进行冷冻 - 融化处理步骤。

6. 发明授权

US07754447B2 Methods for producing recombinant proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：US07754447B2
公开(公告)日：2010-07-13
申请号：US10571367
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/06 , C12P19/00 , C12N13/00
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

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