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1. 再颁专利

USRE44512E1 Methods for producing recombinant proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：USRE44512E1
公开(公告)日：2013-10-01
申请号：US13547892
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/04 , C12N5/00 , C12Q1/68
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

2. 发明授权

US07754447B2 Methods for producing recombinant proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：US07754447B2
公开(公告)日：2010-07-13
申请号：US10571367
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/06 , C12P19/00 , C12N13/00
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

3. 发明申请

US20090324443A1 GAS TREATMENT 审中-公开
标题翻译：气体处理
公开(公告)号：US20090324443A1
公开(公告)日：2009-12-31
申请号：US12278319
申请日：2007-02-19
申请人： John Christopher Whitehead , Kui Zhang , David John Glover
发明人： John Christopher Whitehead , Kui Zhang , David John Glover
IPC分类号： A61L9/16 , B01D53/38 , B01J19/08
CPC分类号： B01D53/32 , B01D53/8668 , B01D2259/818
摘要： In one aspect, the invention provides a gas treatment apparatus (1) comprising a gas-flow path and a plurality of reactor units (5)-(7) through which gas to be treated may flow arranged in series along the path. The reactor units (5)-(7) are adapted to generate a non-equilibrium plasma. This aspect of the invention may be used for decomposing pollutant materials in a gas (e.g. air). When air is being treated, the apparatus of this aspect of the invention is advantageously provided, downstream of the final reactor unit in series, with at least one catalyst bed (8) incorporating a catalyst capable of decomposing ozone. A further aspect of the invention provides apparatus (1) for decomposing a pollutant material dispersed in a gas, the apparatus comprising a gas flow path along which are provided for gas flow therethrough (i) at least one reactor unit (5) which is adapted to generate a non-equilibrium plasma and produce ozone in the gas, and (ii) downstream of (i), at least one catalyst bed (8) incorporating a catalyst capable of decomposing ozone.
摘要翻译：一方面，本发明提供了一种气体处理装置（1），其包括气体流动路径和多个反应器单元（5） - （7），待处理的气体可沿着路径串联排列。反应器单元（5） - （7）适于产生非平衡等离子体。本发明的该方面可用于分解气体（例如空气）中的污染物质。当处理空气时，本发明的这个方面的装置有利地在最终的反应器单元串联的下游与至少一个催化剂床（8）一起提供，该催化剂床结合了能够分解臭氧的催化剂。本发明的另一方面提供了一种用于分解分散在气体中的污染物质的装置（1），该装置包括一气体流动通道，用于气体流过其中的气流（i）至少一个反应器单元（5）产生非平衡等离子体并在气体中产生臭氧，和（ii）下游（i），至少一个催化剂床（8），其结合有能够分解臭氧的催化剂。

4. 发明申请

US20080044817A1 Methods for Producing Recombinant Proteins 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：US20080044817A1
公开(公告)日：2008-02-21
申请号：US10571367
申请日：2004-09-02
申请人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
发明人： David John Glover , Mukesh Sehdev , Dominic Gambier Reeks
IPC分类号： C12P21/04 , C12N5/00 , C12Q1/68
CPC分类号： C12N1/20 , C12P21/02
摘要： The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
摘要翻译：本发明提供了一种用于控制大肠杆菌宿主细胞培养物中上清液和周质之间的重组蛋白质分配的方法，其中所述细胞的重组蛋白质的表达在可诱导系统的控制下，该方法包括：）提供大肠杆菌宿主细胞培养物b）改变大肠杆菌宿主细胞的生长速率c）诱导重组蛋白质的表达，其中步骤（b）和（c）可以以任何顺序或同时进行; 并且随后d）确定培养上清液和大肠杆菌宿主细胞周质中重组蛋白的产量e）将步骤（d）中测定的产量与在步骤中至少一种其他生长速率使用时测定的产量进行比较（ b）f）从步骤（e）中进行的比较中选择生长速率，其中上清液和周质之间的重组蛋白质的分配最适合于重组蛋白质的初级回收。

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