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1. 发明申请

US20090211380A1 Method of Detecting Dispensed Quantity, and Liquid Suction Monitoring Dispensing Apparatus 有权
标题翻译：检测分配量的方法和液体吸引监测分配装置
公开(公告)号：US20090211380A1
公开(公告)日：2009-08-27
申请号：US11920663
申请日：2006-05-19
申请人： Hideji Tajima , Michinori Koizuka , Hiroshi Suzuki , Susumu Kimura
发明人： Hideji Tajima , Michinori Koizuka , Hiroshi Suzuki , Susumu Kimura
IPC分类号： G01N1/14 , B01L3/02
CPC分类号： G01N35/1016 , G01N2035/1025
摘要： There are provided a method of detecting dispensed quantity in which without the need of detecting a surface of liquid accommodated in a container, a liquid quantity can be detected only by measuring pressure change; and a relevant liquid suction monitoring dispensing apparatus. There is provided a method of detecting dispensed quantity for detection of the quantity of liquid suctioned and discharged by a dispensing apparatus including a pipette tip, a liquid suctioning/discharging mechanism for the pipette tip, a pressure sensor capable of detecting the pressure within the pipette tip, and a lifting and lowering mechanism for the pipette tip, which method comprises: an insertion step for inserting a distal end of the pipette tip down to the deepest zone of a container accommodating liquid to be measured; a suction pressure measuring step for without moving of the pipette tip, while suctioning the liquid into the pipette tip at a predetermined suctioning rate, measuring any pressure change during the suctioning; and a suction detecting step for on the basis of pressure change measured, determining the state of suction. Further, there is provided a liquid suction monitoring dispensing apparatus capable of practicing this method.
摘要翻译：提供了一种检测分配量的方法，其中不需要检测容纳在容器中的液体的表面，只能通过测量压力变化来检测液体量; 和相关的液体吸入监测分配装置。提供了一种检测分配量的方法，用于检测由包括移液管尖端的分注装置吸取和排出的液体的量，用于移液管末端的液体吸入/排出机构，能够检测移液管内的压力的压力传感器尖端以及用于吸头的提升和降低机构，该方法包括：插入步骤，用于将移液管尖端的远端向下插入容纳待测量液体的容器的最深处; 抽吸压力测量步骤，用于在移动吸头的同时，以预定的抽吸速率将液体吸入移液管吸头，测量吸力期间的任何压力变化; 以及抽吸检测步骤，用于基于测量的压力变化，确定抽吸状态。此外，提供了能够实施该方法的液体吸引监测分配装置。

2. 发明申请

US20130084606A1 Composition for Preventing Evaporation of Reaction Solution During Nucleic Acid Amplification Reaction 审中-公开
标题翻译：用于防止核酸扩增反应期间反应溶液蒸发的组合物
公开(公告)号：US20130084606A1
公开(公告)日：2013-04-04
申请号：US13703603
申请日：2011-06-22
申请人： Takaki Sugimoto , Tetsuya Ueda , Osamu Segawa , Michinori Koizuka , Hideji Tajima
发明人： Takaki Sugimoto , Tetsuya Ueda , Osamu Segawa , Michinori Koizuka , Hideji Tajima
IPC分类号： C08L91/06 , C12Q1/68 , C12P19/34
CPC分类号： C08L91/06 , B01L3/0275 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2200/0673 , B01L2200/142 , C08L91/00 , C08L2205/02 , C12P19/34 , C12Q1/6846 , C12Q1/6848 , C12Q2547/107 , Y02P20/149
摘要： The present invention provides a composition capable of hermetically sealing a PCR reaction vessel without using a closure member or adhesive seal. Disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, which is a liquid during the reaction and becomes a solid through chemical or thermal changes after completion of the reaction. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 0-15° C. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 5-10° C.
摘要翻译：本发明提供能够不使用封闭构件或粘合剂密封而密封PCR反应容器的组合物。公开了用于在核酸扩增反应期间防止核酸扩增反应溶液蒸发的组合物，其在反应期间为液体，并且在反应完成后通过化学或热变化而变为固体。还公开了用于在核酸扩增反应期间防止核酸扩增反应溶液蒸发的组合物，其中组合物的熔点为0-15℃。还公开了一种用于防止核酸扩增反应溶液蒸发的组合物在核酸扩增反应期间，其中组合物的熔点为5-10℃

3. 发明授权

US08307722B2 Method of detecting dispensed quantity, and liquid suction monitoring dispensing apparatus 有权
标题翻译：检测分配量的方法和液体吸引监测分配装置
公开(公告)号：US08307722B2
公开(公告)日：2012-11-13
申请号：US11920663
申请日：2006-05-19
申请人： Hideji Tajima , Michinori Koizuka , Hiroshi Suzuki , Susumu Kimura
发明人： Hideji Tajima , Michinori Koizuka , Hiroshi Suzuki , Susumu Kimura
IPC分类号： G01N1/14
CPC分类号： G01N35/1016 , G01N2035/1025
摘要： There are provided a method of detecting dispensed quantity in which without the need of detecting a surface of liquid accommodated in a container, a liquid quantity can be detected only by measuring pressure change; and a relevant liquid suction monitoring dispensing apparatus. There is provided a method of detecting dispensed quantity for detection of the quantity of liquid suctioned and discharged by a dispensing apparatus including a pipette tip, a liquid suctioning/discharging mechanism for the pipette tip, a pressure sensor capable of detecting the pressure within the pipette tip, and a lifting and lowering mechanism for the pipette tip, which method comprises: an insertion step for inserting a distal end of the pipette tip down to the deepest zone of a container accommodating liquid to be measured; a suction pressure measuring step for without moving of the pipette tip, while suctioning the liquid into the pipette tip at a predetermined suctioning rate, measuring any pressure change during the suctioning; and a suction detecting step for on the basis of pressure change measured, determining the state of suction. Further, there is provided a liquid suction monitoring dispensing apparatus capable of practicing this method.
摘要翻译：提供了一种检测分配量的方法，其中不需要检测容纳在容器中的液体的表面，只能通过测量压力变化来检测液体量; 和相关的液体吸入监测分配装置。提供了一种检测分配量的方法，用于检测由包括移液管尖端的分注装置吸取和排出的液体的量，用于移液管末端的液体吸引/排出机构，能够检测移液管内的压力的压力传感器尖端以及用于吸头的提升和降低机构，该方法包括：插入步骤，用于将移液管尖端的远端向下插入到容纳被测量液体的容器的最深处; 抽吸压力测量步骤，用于在移动吸头的同时，以预定的抽吸速率将液体吸入移液管吸头，测量吸力期间的任何压力变化; 以及抽吸检测步骤，用于基于测量的压力变化，确定抽吸状态。此外，提供了能够实施该方法的液体吸引监测分配装置。

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