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1. 发明申请

US20060246087A1 Hetero type pentamer recombinant vaccine 失效
标题翻译：异种五聚体重组疫苗
公开(公告)号：US20060246087A1
公开(公告)日：2006-11-02
申请号：US10565595
申请日：2004-07-23
申请人： Takeshi Arakawa , Masanao Kikukawa , Isao Shimabukuro , Masayuki Tadano , Yasunobu Matsumoto , Naotoshi Tsuji , Yoshiya Sato
发明人： Takeshi Arakawa , Masanao Kikukawa , Isao Shimabukuro , Masayuki Tadano , Yasunobu Matsumoto , Naotoshi Tsuji , Yoshiya Sato
IPC分类号： A61K39/12 , C07H21/04 , C12N15/86 , C07K14/18
CPC分类号： A61K39/12 , A61K39/385 , A61K2039/541 , A61K2039/6037 , A61K2039/627 , A61K2039/64 , A61K2039/645 , C07K14/005 , C07K14/245 , C07K14/28 , C07K2319/00 , C07K2319/55 , C07K2319/74 , C12N2770/24122 , C12N2770/24134 , Y02A50/39
摘要： A heteropentamer composed of a fusion monomer (32) comprising a fusion protein of an antigenic amino acid sequence and an amino acid sequence of a monomer of a mucous membrane-binding protein and a nonfusion monomer (20) of an amino acid sequence of a monomer of a mucous membrane-binding protein. A homopentamer composed of a fusion monomer including a fusion protein of an antigen derived from an envelope protein of Japanese encephalitis virus and an amino acid sequence of a monomer of a mucous membrane-binding protein. These heteropentamer and the homopentamer can be used as a vaccine.
摘要翻译：由包含抗原性氨基酸序列的融合蛋白和粘膜结合蛋白的单体的氨基酸序列和单体的氨基酸序列的非融合单体（20）的融合单体（32）组成的异戊二敏单体的粘膜结合蛋白。由包含衍生自日本脑炎病毒的包膜蛋白的抗原的融合蛋白和粘膜结合蛋白的单体的氨基酸序列的融合单体组成的同销口。这些异戊酸和异戊酸可以用作疫苗。

2. 发明授权

US07544361B2 Hetero type pentamer recombinant vaccine 失效
标题翻译：异种五聚体重组疫苗
公开(公告)号：US07544361B2
公开(公告)日：2009-06-09
申请号：US10565595
申请日：2004-07-23
申请人： Takeshi Arakawa , Masanao Kikukawa , Isao Shimabukuro , Masayuki Tadano , Yasunobu Matsumoto , Naotoshi Tsuji , Yoshiya Sato
发明人： Takeshi Arakawa , Masanao Kikukawa , Isao Shimabukuro , Masayuki Tadano , Yasunobu Matsumoto , Naotoshi Tsuji , Yoshiya Sato
IPC分类号： A61K39/385 , A61K39/12 , A61K39/295 , A61K9/28 , C07K19/00 , C07K17/00 , C12N15/62 , C12N15/63 , C12N1/21
CPC分类号： A61K39/12 , A61K39/385 , A61K2039/541 , A61K2039/6037 , A61K2039/627 , A61K2039/64 , A61K2039/645 , C07K14/005 , C07K14/245 , C07K14/28 , C07K2319/00 , C07K2319/55 , C07K2319/74 , C12N2770/24122 , C12N2770/24134 , Y02A50/39
摘要： A heteropentamer composed of a fusion monomer (32) comprising a fusion protein of an antigenic amino acid sequence and an amino acid sequence of a monomer of a mucous membrane-binding protein and a nonfusion monomer (20) of an amino acid sequence of a monomer of a mucous membrane-binding protein. A homopentamer composed of a fusion monomer including a fusion protein of an antigen derived from an envelope protein of Japanese encephalitis virus and an amino acid sequence of a monomer of a mucous membrane-binding protein. These heteropentamer and the homopentamer can be used as a vaccine.
摘要翻译：由包含抗原性氨基酸序列的融合蛋白和粘膜结合蛋白的单体的氨基酸序列和单体的氨基酸序列的非融合单体（20）的融合单体（32）组成的异戊二敏单体的粘膜结合蛋白。由包含源自日本脑炎病毒的包膜蛋白的抗原的融合蛋白和粘膜结合蛋白的单体的氨基酸序列的融合单体构成的同销口。这些异戊酸和异戊酸可以用作疫苗。

3. 发明授权

US06773913B2 Device and process for purifying vectors 失效
标题翻译：用于纯化载体的装置和方法
公开(公告)号：US06773913B2
公开(公告)日：2004-08-10
申请号：US09964377
申请日：2001-09-28
申请人： Sakae Arakaki , Masaru Nagamine , Masanao Kikukawa
发明人： Sakae Arakaki , Masaru Nagamine , Masanao Kikukawa
IPC分类号： C12M112
CPC分类号： C12N15/1017
摘要： In a vector purification device, bacteria containing vectors are cultured in a culture medium in a culture tank, the culture medium is concentrated by filtration with a first TFF film, and the concentrated culture medium is replaced with a buffer solution by filtering a mixture of the culture medium and the buffer solution by the TFF film while supplying the buffer solution from a buffer tank. The bacteria subjected to bacteriolysis by an alkali solution supplied from an alkali tank are passed through a second TFF film to remove undesired substances. Since the TFF films are used in place of a centrifugal separator for concentration or the like, a series of operations can be carried out continuously in a closed system. Further, a crudely purified vector solution is treated with a surfactant to separate vector DNAs from endotoxins as separate layers, and the endotoxins are removed. According to the device, a series of operations such as concentration of the culture medium can be carried out continuously in a closed system, and high-purity vectors can be obtained.
摘要翻译：在载体纯化装置中，将含细菌的载体培养在培养槽中的培养基中，用第一TFF膜过滤浓缩培养基，用缓冲液置换浓缩培养基，培养基和缓冲溶液，同时从缓冲罐供给缓冲溶液。经由碱性槽供给的碱溶液进行细菌溶解的细菌通过第二TFF膜以除去不需要的物质。由于使用TFF膜代替用于浓缩等的离心分离器，因此可以在封闭系统中连续进行一系列操作。此外，将粗制纯化的载体溶液用表面活性剂处理以将来自内毒素的载体DNA作为单独的层分离，并除去内毒素。根据该装置，可以在封闭系统中连续进行培养液浓缩等一系列操作，可以得到高纯度的载体。

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