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1. 发明申请

US20110183371A1 MICROBE-COLLECTING CARRIER CARTRIDGE, CARRIER TREATING APPARATUS, AND METHOD OF MEASURING MICROBES 有权
标题翻译： MICROBE收集载体盒，载体处理装置和测量微阵列的方法
公开(公告)号：US20110183371A1
公开(公告)日：2011-07-28
申请号：US13001465
申请日：2009-06-25
申请人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
发明人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
IPC分类号： C12Q1/06 , C12M1/00 , C12M1/34
CPC分类号： C12Q1/008 , B01L3/502 , B01L2300/0681 , B01L2300/069 , B01L2300/1805 , B01L2400/0487 , G01N1/2208 , G01N1/2273
摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, such as gelatin, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped container and is stored in the liquid storage sink, thereby causing the carrier to make contact with the warm water. The contact with the warm water causes the carrier to be solated, and mixed and diluted with the warm water. Then, the suction pump of the filtration device is activated for suction and filtration of the mixed liquid through the filter. By using the filter having a shape not allowing microbes to pass through and having a gap, only microbes are captured on the filter after suction and filtration. Polymers and dust to be a background upon an ATP measurement pass through the filter by filtration to be cleanly eliminated.
摘要翻译：提供一种一次性微生物收集载体盒和载体处理装置，其将收集在载体上的微生物有效地转化为溶液并防止在过滤器上浓缩时微生物的回收量的变化。由具有多个通孔的上盖形成的盒，具有倒置锥形的容器，其具有在底部具有过滤器的液体储存槽单元和空气吸入路径，以及用于设置制备诸如明胶的热塑性载体，以及由用于液体供应的分配喷嘴形成的载体处理装置，液体供给机构，液体加热机构和用于过滤的抽吸泵。向设置在倒锥形容器的底面上的过滤器供给温水，并将其储存在液体储存槽中，从而使载体与温水接触。与温水接触导致载体被溶解，并与温水混合并稀释。然后，过滤装置的抽吸泵被激活，以通过过滤器抽吸和过滤混合液体。通过使用具有不允许微生物通过并具有间隙的形状的过滤器，在抽吸和过滤之后仅在过滤器上捕获微生物。聚合物和粉尘作为ATP测量的背景，通过过滤通过过滤器，以便清洁地消除。

2. 发明申请

US20110176975A1 REAGENT SPLITTING / DISPENSING METHOD BASED ON REAGENT DISPENSING NOZZLE AND REAGENT SPLITTING / DISPENSING MECHANISM 失效
标题翻译：试剂分选/分配方法基于试剂分配喷嘴和试剂分选/分配机构
公开(公告)号：US20110176975A1
公开(公告)日：2011-07-21
申请号：US13009037
申请日：2011-01-19
申请人： Noe MIYASHITA , Hideyuki Noda , Masahiro Okanojo
发明人： Noe MIYASHITA , Hideyuki Noda , Masahiro Okanojo
IPC分类号： B01L3/00
CPC分类号： B01L3/52 , F17D3/00 , G01N21/763 , G01N35/1002 , G01N35/1011 , G01N2035/102 , G01N2035/1032 , Y10T137/0324 , Y10T436/118339 , Y10T436/119163 , Y10T436/2575
摘要： The present invention provides a reagent splitting/dispensing method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet. The reagent splitting/dispensing method includes a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle, a first moving process of moving the reagent dispensing nozzle to the position right above the reagent, a second moving process of depositing the nozzle tip end in the reagent, a reagent splitting process of filling the reagent into the reagent dispensing nozzle, a third moving process of evacuating the tip end of the reagent dispensing nozzle from the reagent, a reagent protecting process of disposing a second air layer in the nozzle tip end, a fourth moving process of moving the reagent dispensing nozzle to the reagent dispensation position, a reagent dispensing process of ejecting the split reagent, an operation fluid protecting process of disposing the first air layer in the nozzle tip end, after dispensing the reagent, and a fifth moving process of evacuating the reagent dispensing nozzle to the waiting position, in a state where the first air layer is disposed in the nozzle tip end.
摘要翻译：本发明提供一种试剂分注方法，其能够防止试剂分注喷嘴处于等待状态时的工作液的污染，防止液滴的下降。试剂分注/分注方法包括在试剂分注喷嘴的操作流体的界面和喷嘴前端之间设置第一空气层的等待处理，将试剂分注喷嘴移动到试剂，将试剂分注喷嘴的前端从试剂抽出的第三移动处理，将试剂分注喷嘴从试剂抽出的第三移动处理，在喷嘴前端设置第二空气层的保护过程，将试剂分配喷嘴移动到试剂分配位置的第四移动处理，喷射分离试剂的试剂分注处理，将第一空气配置的操作流体保护处理在分配试剂之后的喷嘴尖端中的层，以及抽空试剂分配喷嘴的第五移动过程 e到第一空气层设置在喷嘴前端的状态下的等待位置。

3. 发明授权

US09834806B2 Microbe-collecting carrier cartridge, carrier treating apparatus, and method of measuring microbes 有权
公开(公告)号：US09834806B2
公开(公告)日：2017-12-05
申请号：US13001465
申请日：2009-06-25
申请人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
发明人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/06 , C12Q1/00 , B01L3/00 , G01N1/22
CPC分类号： C12Q1/008 , B01L3/502 , B01L2300/0681 , B01L2300/069 , B01L2300/1805 , B01L2400/0487 , G01N1/2208 , G01N1/2273
摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped. The contact with the warm water causes the carrier to be solated filtered through the filter.

4. 发明授权

US08628953B2 Capturing carrier, capturing device, analysis system using the same, and method for capturing and testing microorganisms 有权
标题翻译：捕获载体，捕获装置，使用其的分析系统以及捕获和测试微生物的方法
公开(公告)号：US08628953B2
公开(公告)日：2014-01-14
申请号：US12292626
申请日：2008-11-21
申请人： Noe Osato , Ryusuke Gotoda , Satoshi Ozawa , Hideyuki Noda , Masahiro Okanojo
发明人： Noe Osato , Ryusuke Gotoda , Satoshi Ozawa , Hideyuki Noda , Masahiro Okanojo
IPC分类号： C12M1/00
CPC分类号： C12Q1/04 , G01N1/2208 , G01N1/2214 , G01N2001/4088
摘要： In order to make a process of capturing and testing air-borne microorganisms more convenient and quick, a capturing device which comprises the capturing carrier comprising a polymer which is in a gel phase at the time of capturing the microorganisms but undergoes a phase transition under heating to a sol phase at the temperature at or less than 40° C. (especially in the temperature range between 15° C. and 37° C.), and a vessel to contain the capturing carrier is used. Further, by comprising a test reagent in the polymer, the test reagent can be eluted upon said phase transition from a gel phase to a sol phase. By heating the capturing carrier, a phase transition to a sol phase can occur at or less than 40° C. As such, since recovery of microorganisms and addition of a test reagent to the microorganisms can be carried out simultaneously, the process can be simplified and the process time can be shortened.
摘要翻译：为了更方便快捷地捕获和测试空气传播的微生物的过程，捕获装置包括捕获载体，捕获载体包含在捕获微生物时处于凝胶相的聚合物，但在加热下经历相变在等于或低于40℃的温度下（特别是在15℃至37℃的温度范围内）的溶胶相，并且使用容纳捕获载体的容器。此外，通过在聚合物中包含测试试剂，在从凝胶相到溶胶相的所述相转变时，可以洗脱测试试剂。通过加热捕获载体，可以在40℃以下发生溶胶相的相变。因此，由于可以同时进行微生物的回收和微生物的添加试验，因此可以简化该过程并且可以缩短处理时间。

5. 发明授权

US08333936B2 Reagent splitting/dispensing method based on reagent dispensing nozzle and reagent splitting/dispensing mechanism 失效
标题翻译：基于试剂分配喷嘴和试剂分配/分配机构的试剂分流/分液方法
公开(公告)号：US08333936B2
公开(公告)日：2012-12-18
申请号：US13009037
申请日：2011-01-19
申请人： Noe Miyashita , Hideyuki Noda , Masahiro Okanojo
发明人： Noe Miyashita , Hideyuki Noda , Masahiro Okanojo
IPC分类号： B01L3/02 , G01N1/10
CPC分类号： B01L3/52 , F17D3/00 , G01N21/763 , G01N35/1002 , G01N35/1011 , G01N2035/102 , G01N2035/1032 , Y10T137/0324 , Y10T436/118339 , Y10T436/119163 , Y10T436/2575
摘要： The present invention provides for a reagent splitting/dispensing device and method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet. The reagent splitting/dispensing method includes disposing and adjusting an air layer between an interface of an operation fluid and a reagent aspirated and subsequently dispensed from a nozzle tip end in an reagent dispensing nozzle.
摘要翻译：本发明提供了一种试剂分配/分配装置和方法，其可以防止当试剂分配喷嘴处于等待状态时操作流体的污染并防止液滴的下落。试剂分注/分配方法包括在操作流体的界面和吸附的试剂之间布置和调节空气层，并随后从试剂分配喷嘴中的喷嘴尖端分配。

6. 发明申请

US20120250024A1 LUMINESCENCE MEASURING APPARATUS AND MICROBE COUNTING APPARATUS 审中-公开
标题翻译：发光测量装置和微型计数装置
公开(公告)号：US20120250024A1
公开(公告)日：2012-10-04
申请号：US13425497
申请日：2012-03-21
申请人： Hideyuki NODA , Masahiro Okanojo
发明人： Hideyuki NODA , Masahiro Okanojo
IPC分类号： G01B9/02 , G01J1/00
CPC分类号： G01N21/6486 , G01N21/76 , G02B21/16
摘要： The present invention relates to a luminescence measuring apparatus for detecting, at high sensitivity and high accuracy, chemoluminescence and bioluminescence of a substance contained in a sample. Specifically, the present invention provides an apparatus for measuring an amount of luminescence in a sample, comprising: a container for the sample; a photodetector for detecting luminescence from the container; and at least one optical filter inserted between the photodetector and the container, and/or a pH modifier added to the container, wherein the photodetector performs measurement of light emitted from the container at all wavelength regions and spectrometry at a specific wavelength range, and/or measurement of light, the intensity of which is changed by the pH modifier. The present invention also relates to a microbe counting apparatus based on luminescence detection of ATP in a viable microbial cell.
摘要翻译：本发明涉及用于以高灵敏度和高精度检测样品中所含物质的化学发光和生物发光的发光测量装置。具体而言，本发明提供了一种用于测量样品中的发光量的装置，包括：用于样品的容器; 用于检测来自容器的发光的光电检测器; 以及至少一个插入到光电检测器和容器之间的滤光器，和/或添加到容器中的pH调节剂，其中光电检测器在所有波长区域进行从容器发射的光的测量和在特定波长范围的光谱测定，和/ 或光的测量，其强度由pH调节剂改变。本发明还涉及一种基于活的微生物细胞中ATP的发光检测的微生物计数装置。

7. 发明申请

US20120231493A1 APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION 有权
标题翻译：用于荧光测定和检测的装置
公开(公告)号：US20120231493A1
公开(公告)日：2012-09-13
申请号：US13478527
申请日：2012-05-23
申请人： Hideyuki NODA , Satoshi OZAWA , Masahiro OKANOJO , Kenko UCHIDA
发明人： Hideyuki NODA , Satoshi OZAWA , Masahiro OKANOJO , Kenko UCHIDA
IPC分类号： G01N21/76
CPC分类号： C12Q1/04 , G01N21/76
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译：一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

8. 发明申请

US20080261294A1 APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION 有权
标题翻译：用于荧光测定和检测的装置
公开(公告)号：US20080261294A1
公开(公告)日：2008-10-23
申请号：US12034880
申请日：2008-02-21
申请人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
IPC分类号： G01N21/76 , C12M1/36
CPC分类号： C12Q1/04 , G01N21/76
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译：一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。虽然没有实现测量，但是关闭快门单元以阻止杂散光入射到光电检测器，并且在实施测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入通孔使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

9. 发明申请

US20130139898A1 REAGENT SPLITTING/DISPENSING METHOD BASED ON REAGENT DISPENSING NOZZLE AND REAGENT SPLITTING/DISPENSING MECHANISM 审中-公开
标题翻译：试剂分选/分配方法基于试剂分配喷嘴和试剂分选/分配机构
公开(公告)号：US20130139898A1
公开(公告)日：2013-06-06
申请号：US13677462
申请日：2012-11-15
申请人： Noe Miyashita , Hideyuki Noda , Masahiro Okanojo
发明人： Noe Miyashita , Hideyuki Noda , Masahiro Okanojo
IPC分类号： B01L3/00 , F17D3/00
CPC分类号： B01L3/52 , F17D3/00 , G01N21/763 , G01N35/1002 , G01N35/1011 , G01N2035/102 , G01N2035/1032 , Y10T137/0324 , Y10T436/118339 , Y10T436/119163 , Y10T436/2575
摘要： A reagent splitting/dispensing method based on a reagent dispensing nozzle controls a split or dispensation amount of reagent, includes a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle; a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split; a second moving process of depositing the nozzle tip end in the reagent; an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process; and a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end.
摘要翻译：基于试剂分注喷嘴的试剂分注/分配方法控制分液或分配量的试剂，包括在操作流体的界面和试剂分配喷嘴中的喷嘴前端之间设置第一空气层的等待过程; 将试剂分注喷嘴移动到待分离试剂上方的位置的第一移动处理; 将所述喷嘴前端沉积在所述试剂中的第二移动过程; 在所述第一移动处理和所述第二移动处理之间增加所述试剂分注用喷嘴中的操作流体的占有量并减少所述第一空气层的占有量的空气层调整处理; 以及试剂分解处理，其减少试剂分注喷嘴中的操作流体的占有量并将试剂从喷嘴前端填充到试剂分注喷嘴中。

10. 发明申请

US20120321520A1 LIQUID SUCTION DEVICE 有权
标题翻译：液体吸收装置
公开(公告)号：US20120321520A1
公开(公告)日：2012-12-20
申请号：US13580674
申请日：2010-12-22
申请人： Masahiro Okanojo , Hideyuki Noda
发明人： Masahiro Okanojo , Hideyuki Noda
IPC分类号： G01N1/14 , G01N21/64
CPC分类号： G01N35/1011 , G01N21/6428
摘要： The present invention enables liquid suction with a nozzle tip end in contact with a bottom surface of a container without causing damage to the nozzle and/or the bottom surface of the container.Plural rails 112 are disposed on an arm 101 movable three-dimensionally so as to extend upward from the arm 101, and a pipe 102 is fixed to a pipe fixing member 103 movable along the rails. A tip end of the pipe functions as a nozzle. Plural resilient members 114 are disposed on the arm so as to correct the inclination of the pipe fixing member. When the arm is moved downward and the tip end of the pipe comes in contact with the bottom of the container 106, the pipe and the pipe fixing portion move upward along the rails, so that the tip end of the pipe can be brought into contact with the bottom surface of the container while avoiding damages in the pipe and bottom of the container and suck liquid in the container in this state.
摘要翻译：本发明能够使喷嘴前端的液体吸附与容器的底面接触而不会损坏容器的喷嘴和/或底面。多个轨道112设置在能够从臂101上向三维移动的臂101上，并且管102固定到沿着轨道移动的管固定构件103。管的尖端用作喷嘴。多个弹性构件114设置在臂上，以便校正管固定构件的倾斜度。当臂向下移动并且管的顶端与容器106的底部接触时，管和管固定部沿着轨道向上移动，使得管的顶端可以接触同时避免容器的管道和底部的损坏，并且在该状态下吸入容器中的液体。

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