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1. 发明授权

US09834806B2 Microbe-collecting carrier cartridge, carrier treating apparatus, and method of measuring microbes 有权
公开(公告)号：US09834806B2
公开(公告)日：2017-12-05
申请号：US13001465
申请日：2009-06-25
申请人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
发明人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/06 , C12Q1/00 , B01L3/00 , G01N1/22
CPC分类号： C12Q1/008 , B01L3/502 , B01L2300/0681 , B01L2300/069 , B01L2300/1805 , B01L2400/0487 , G01N1/2208 , G01N1/2273
摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped. The contact with the warm water causes the carrier to be solated filtered through the filter.

2. 发明申请

US20110183371A1 MICROBE-COLLECTING CARRIER CARTRIDGE, CARRIER TREATING APPARATUS, AND METHOD OF MEASURING MICROBES 有权
标题翻译： MICROBE收集载体盒，载体处理装置和测量微阵列的方法
公开(公告)号：US20110183371A1
公开(公告)日：2011-07-28
申请号：US13001465
申请日：2009-06-25
申请人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
发明人： Hideyuki Noda , Masahiro Okanojo , Kenko Uchida , Norihito Kuno
IPC分类号： C12Q1/06 , C12M1/00 , C12M1/34
CPC分类号： C12Q1/008 , B01L3/502 , B01L2300/0681 , B01L2300/069 , B01L2300/1805 , B01L2400/0487 , G01N1/2208 , G01N1/2273
摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, such as gelatin, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped container and is stored in the liquid storage sink, thereby causing the carrier to make contact with the warm water. The contact with the warm water causes the carrier to be solated, and mixed and diluted with the warm water. Then, the suction pump of the filtration device is activated for suction and filtration of the mixed liquid through the filter. By using the filter having a shape not allowing microbes to pass through and having a gap, only microbes are captured on the filter after suction and filtration. Polymers and dust to be a background upon an ATP measurement pass through the filter by filtration to be cleanly eliminated.
摘要翻译：提供一种一次性微生物收集载体盒和载体处理装置，其将收集在载体上的微生物有效地转化为溶液并防止在过滤器上浓缩时微生物的回收量的变化。由具有多个通孔的上盖形成的盒，具有倒置锥形的容器，其具有在底部具有过滤器的液体储存槽单元和空气吸入路径，以及用于设置制备诸如明胶的热塑性载体，以及由用于液体供应的分配喷嘴形成的载体处理装置，液体供给机构，液体加热机构和用于过滤的抽吸泵。向设置在倒锥形容器的底面上的过滤器供给温水，并将其储存在液体储存槽中，从而使载体与温水接触。与温水接触导致载体被溶解，并与温水混合并稀释。然后，过滤装置的抽吸泵被激活，以通过过滤器抽吸和过滤混合液体。通过使用具有不允许微生物通过并具有间隙的形状的过滤器，在抽吸和过滤之后仅在过滤器上捕获微生物。聚合物和粉尘作为ATP测量的背景，通过过滤通过过滤器，以便清洁地消除。

3. 发明申请

US20050123965A1 RNA extraction method, RNA extraction reagent, and method for analyzing biological materials 审中-公开
标题翻译： RNA提取法，RNA提取试剂，生物材料分析方法等
公开(公告)号：US20050123965A1
公开(公告)日：2005-06-09
申请号：US10981521
申请日：2004-11-05
申请人： Yoshihiro Yamashita , Toshinari Sakurai , Norihito Kuno , Kenko Uchida , Toshiaki Yokobayashi
发明人： Yoshihiro Yamashita , Toshinari Sakurai , Norihito Kuno , Kenko Uchida , Toshiaki Yokobayashi
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/1006 , C12N15/101
摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.
摘要翻译：提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。

4. 发明授权

US07737268B2 Method of recovering nucleic acids and kit for recovering nucleic acids 有权
标题翻译：用于回收核酸的方法和用于回收核酸的试剂盒
公开(公告)号：US07737268B2
公开(公告)日：2010-06-15
申请号：US10848368
申请日：2004-05-19
申请人： Norihito Kuno , Kenko Uchida
发明人： Norihito Kuno , Kenko Uchida
IPC分类号： C07H21/00 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/1006
摘要： Disclosure is a method of separately recovering deoxyribonucleic acid and ribonucleic acid from a biological sample solution. They are separated and recovered from a single biological sample by controlling pH values, temperature, and/or concentration of cationic ions of a nucleic acid sample solution containing a caotropic agent. It is possible to easily and safely separate and recover deoxyribonucleic acid and ribonucleic acid from the single biological sample.
摘要翻译：披露是从生物样品溶液中分离回收脱氧核糖核酸和核糖核酸的方法。通过控制含有约束剂的核酸样品溶液的阳离子离子的pH值，温度和/或浓度，从单个生物样品中分离和回收它们。可以从单一生物样品中容易且安全地分离和回收脱氧核糖核酸和核糖核酸。

5. 发明申请

US20090111114A1 RNA EXTRACTION METHOD, RNA EXTRACTION REAGENT, AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS 审中-公开
标题翻译： RNA提取方法，RNA提取试剂和分析生物材料的方法
公开(公告)号：US20090111114A1
公开(公告)日：2009-04-30
申请号：US12251375
申请日：2008-10-14
申请人： Yoshihiro Yamashita , Toshinari Sakurai , Norihito Kuno , Kenko Uchida , Toshiaki Yokobayashi
发明人： Yoshihiro Yamashita , Toshinari Sakurai , Norihito Kuno , Kenko Uchida , Toshiaki Yokobayashi
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/101
摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.
摘要翻译：提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。

6. 发明申请

US20050288867A1 Cell tissue culture management method and system 审中-公开
标题翻译：细胞组织培养管理方法和系统
公开(公告)号：US20050288867A1
公开(公告)日：2005-12-29
申请号：US11166095
申请日：2005-06-27
申请人： Norihito Kuno
发明人： Norihito Kuno
IPC分类号： C12M3/00 , C12N5/07 , C12N5/071 , C12N15/09 , C12Q1/68 , G01N33/48 , G06F19/00 , G06F19/28
CPC分类号： C12M99/00
摘要： In order to detect the mis-operation of cells or tissues in a culturing step and other working steps to prevent erroneous use in treatment of cultured cell and tissues besides those of the cell donor, a genetic information, held by a cell or tissue, is used as a cell tissue information, which is inseparable from the cell tissue itself and enables individual identification of the cell donor, that is, identification of the origin of the cell or tissue subject to a culture process, and the cell tissue information before and after culturing are compared and collated.
摘要翻译：为了检测培养步骤中的细胞或组织的错误操作以及除了细胞供体以外的培养细胞和组织的治疗中的错误使用的其他工作步骤，由细胞或组织保持的遗传信息是用作细胞组织信息，其与细胞组织本身是不可分离的，并且能够单独鉴定细胞供体，即鉴定受培养过程的细胞或组织的来源，以及前后的细胞组织信息比较和整理培养。

7. 发明申请

US20100055779A1 CELL CULTURE VESSEL, PRODUCTION PROCESS THEREOF AND CULTURED CELL 有权
标题翻译：细胞培养血管，其生产过程和培养细胞
公开(公告)号：US20100055779A1
公开(公告)日：2010-03-04
申请号：US12614471
申请日：2009-11-09
申请人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
发明人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
IPC分类号： C12N5/00 , B29C43/40 , B29C43/50 , A61L2/04
CPC分类号： C12M23/10 , C12M25/00 , C12M25/06
摘要： An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.
摘要翻译：本发明的目的是提供一种结构简单，易于处理的细胞培养容器，能够在分离时防止细胞损伤，促进营养物质的运输和有效物质的排出，提高培养效率改善结构特点的效果。为了实现上述目的，提供了一种细胞培养容器，其包括具有等效直径小于待培养细胞的多个突起的培养部分和包围培养部分的培养部分侧壁，在培养部/侧壁边界线上的任意位置与最近的突起之间的距离小于待培养细胞的直径。提供给培养细胞的血管中的突起的作用增强。

8. 发明申请

US20060281172A1 Cell culturel vessel, production process thereof and cultured cell 有权
标题翻译：细胞培养容器，其生产过程和培养细胞
公开(公告)号：US20060281172A1
公开(公告)日：2006-12-14
申请号：US11441235
申请日：2006-05-26
申请人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
发明人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
IPC分类号： C12M1/22
CPC分类号： C12M23/10 , C12M25/00 , C12M25/06
摘要： An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.
摘要翻译：本发明的目的是提供一种结构简单，易于处理的细胞培养容器，能够在分离时防止细胞损伤，促进营养物质的运输和有效物质的排出，提高培养效率改善结构特点的效果。为了实现上述目的，提供了一种细胞培养容器，其包括具有等效直径小于待培养细胞的多个突起的培养部分和包围培养部分的培养部分侧壁，在培养部/侧壁边界线上的任意位置与最近的突起之间的距离小于待培养细胞的直径。提供给培养细胞的血管中的突起的作用增强。

9. 发明申请

US20060115381A1 Solution mixing device and analysis system 审中-公开
标题翻译：溶液混合装置及分析系统
公开(公告)号：US20060115381A1
公开(公告)日：2006-06-01
申请号：US11195665
申请日：2005-08-03
申请人： Norihito Kuno , Masayoshi Ishibashi
发明人： Norihito Kuno , Masayoshi Ishibashi
IPC分类号： B01L3/00
CPC分类号： B01F11/0045 , B01F13/0059 , B01L3/502 , B01L3/5027 , B01L2300/0636 , B01L2300/0822 , B01L2400/0481
摘要： An analyzer to carry out a reaction with efficiency is provided. A substrate and a cover member having a concave portion to form a space for retaining a reaction solution are constructed of deformable material, the cover member is deformed by exerting a force externally of the cover member, and the reaction solution introduced into the space for reaction is moved within the space by this deformation, thereby allowing the reaction solution to be stirred within the space. Enhancement of signal intensities is achieved by improvement of reaction efficiency due to mixing. Further, uniform mixing can be achieved over the entire region within the space because an arbitrary location of the cover member can be deformed.
摘要翻译：提供了一种能够有效地进行反应的分析仪。具有凹部以形成用于保持反应溶液的空间的基板和盖构件由可变形材料构成，盖构件通过在盖构件外部施加力而变形，并将反应溶液引入反应空间通过该变形在空间内移动，从而使反应溶液在空间内被搅拌。通过改善由于混合引起的反应效率来实现信号强度的提高。此外，可以在空间内的整个区域上实现均匀的混合，因为盖构件的任意位置可能变形。

10. 发明授权

US07888111B2 Cell culture vessel, production process thereof and cultured cell 有权
标题翻译：细胞培养容器，其生产过程和培养细胞
公开(公告)号：US07888111B2
公开(公告)日：2011-02-15
申请号：US12614471
申请日：2009-11-09
申请人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
发明人： Kosuke Kuwabara , Akihiro Miyauchi , Norihito Kuno
IPC分类号： C12M1/14 , C12M3/04 , C12M3/00 , C12M1/00
CPC分类号： C12M23/10 , C12M25/00 , C12M25/06
摘要： An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.
摘要翻译：本发明的目的是提供一种结构简单，易于处理的细胞培养容器，能够在分离时防止细胞损伤，促进营养物质的运输和有效物质的排出，提高培养效率改善结构特点的效果。为了实现上述目的，提供了一种细胞培养容器，其包括具有等效直径小于待培养细胞的多个突起的培养部分和包围培养部分的培养部分侧壁，在培养部/侧壁边界线上的任意位置与最近的突起之间的距离小于待培养细胞的直径。提供给培养细胞的血管中的突起的作用增强。

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