专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP1501351A2 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 审中-公开
标题翻译：同源短片段替代GENERATING疯牛病牛耐
公开(公告)号：EP1501351A2
公开(公告)日：2005-02-02
申请号：EP03741764.9
申请日：2003-04-17
申请人： Metz, Richard , Blaese, Michael R.
发明人： Metz, Richard , Blaese, Michael R.
IPC分类号： A01K67/027
CPC分类号： A01K67/0275 , A01K2227/101 , A01K2267/02 , C12N15/8509 , C12N15/8771 , C12N2790/10011 , C12N2790/10022
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alteration in the PrP gene is disclosed. The PrP gene of a cultured cell is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.

2. 发明专利

AU2003291073A8 A method for cloning of a rare, specifically mutated cell 未知
公开(公告)号：AU2003291073A8
公开(公告)日：2009-07-30
申请号：AU2003291073
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： BLAESE MICHAEL R , DICOLA MIKE , METZ RICHARD
IPC分类号： C12Q1/68 , C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/82 , C12N15/85

3. 发明专利

AU2003291073A1 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 未知
公开(公告)号：AU2003291073A1
公开(公告)日：2004-06-15
申请号：AU2003291073
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
IPC分类号： C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/85 , C12Q1/68

4. 发明专利

AU2003262386A1 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 未知
公开(公告)号：AU2003262386A1
公开(公告)日：2003-11-03
申请号：AU2003262386
申请日：2003-04-17
申请人： BLAESE R MICHAEL , METZ RICHARD
发明人： METZ RICHARD , BLAESE MICHAEL R
IPC分类号： A01K67/027 , A61K48/00 , C12N20060101 , C12N15/09 , C12N15/85 , C12N15/877 , C12Q1/68
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alterations in the PrP gene is disclosed. The PrP gene of a cultured cells is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.

5. 发明申请

WO2004046335A2 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 审中-公开
标题翻译：用于克隆稀有特异性突变细胞的方法
公开(公告)号：WO2004046335A2
公开(公告)日：2004-06-03
申请号：PCT/US2003/036882
申请日：2003-11-18
申请人： METZ, Richard , DICOLA, Mike , BLAESE, Michael, R.
发明人： METZ, Richard , DICOLA, Mike , BLAESE, Michael, R.
IPC分类号： C12N
CPC分类号： C12N15/1034
摘要： The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration. The method comprises sequentially using polymerase chain reaction (PCR) using an allele specific primer to preferentially amplify sequences containing one of the two linked alterations, coupled with a second technique that detects the second change in the PCR product. The second method may comprise restriction digestion, conventional DNA sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected using the methods provided.
摘要翻译：本发明涉及一种检测培养细胞的定向遗传改变的稀有产物的新方法。该方法适用于任何改变方法。该方法包括使用等位基因特异性引物依次使用聚合酶链反应（PCR）来优先扩增含有两个连接的改变之一的序列，加上检测PCR产物的第二个变化的第二个技术。第二种方法可以包括限制性消化，常规DNA测序或热解测序。实验表明，使用所提供的方法可以检测到10,000个细胞中一个正确改变的拷贝稀少的改变。

6. 发明申请

WO2004046335A3 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 审中-公开
标题翻译：用于克隆稀有特异性突变细胞的方法
公开(公告)号：WO2004046335A3
公开(公告)日：2009-06-18
申请号：PCT/US0336882
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
IPC分类号： C12Q1/68 , C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/82 , C12N15/85
CPC分类号： C12N15/1034
摘要： The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration. The method comprises sequentially using polymerase chain reaction (PCR) using an allele specific primer to preferentially amplify sequences containing one of the two linked alterations, coupled with a second technique that detects the second change in the PCR product. The second method may comprise restriction digestion, conventional DNA sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected using the methods provided.
摘要翻译：本发明涉及一种检测培养细胞的定向遗传改变的稀有产物的新方法。该方法适用于任何改变方法。该方法包括使用等位基因特异性引物依次使用聚合酶链反应（PCR）来优先扩增含有两个连接的改变之一的序列，加上检测PCR产物的第二个变化的第二个技术。第二种方法可以包括限制性消化，常规DNA测序或热解测序。实验表明，使用所提供的方法可以检测到10,000个细胞中一个正确改变的拷贝稀少的改变。

7. 发明申请

WO2008143668A3 INDOLEAMINE-2, 3-DIOXYGENASE-2 审中-公开
标题翻译： INDOLEAMINE-2，3-DIOXYGENASE-2
公开(公告)号：WO2008143668A3
公开(公告)日：2009-03-26
申请号：PCT/US2007069271
申请日：2007-05-18
申请人： LANKENAU INST MEDICAL RES , PRENDERGAST GEORGE C , METZ RICHARD
发明人： PRENDERGAST GEORGE C , METZ RICHARD
IPC分类号： C07K2/00
CPC分类号： C12N9/0069 , A01K67/0276 , C07K2319/21 , C07K2319/40 , C12N2830/006 , C12Q1/26
摘要： The nucleotide and amino acid sequences of indoleamine 2, 3-dioxygenase-2 (ID02) and methods of use thereof are provided.
摘要翻译：提供了吲哚胺2,3-双加氧酶-2（ID02）的核苷酸和氨基酸序列及其使用方法。

8. 发明申请

WO2009003164A1 COMPOSITIONS COMPRISING INDOLEAMINE 2,3- DIOXYGENASE-2 AND COENZYME Q INHIBITORS AND METHODS OF USE THEREOF 审中-公开
标题翻译：包含吲哚胺2,3-二氧杂环己烷-2和聚苯乙烯Q抑制剂的组合物及其使用方法
公开(公告)号：WO2009003164A1
公开(公告)日：2008-12-31
申请号：PCT/US2008/068557
申请日：2008-06-27
申请人： LANKENAU INSTITUTE FOR MEDICAL RESEARCH DEVELOPMENT, INC. , PRENDERGAST, George, C. , METZ, Richard
发明人： PRENDERGAST, George, C. , METZ, Richard
IPC分类号： A01N43/40 , A61K31/44 , A61K38/43
CPC分类号： A61K31/44 , A61K31/122 , A61K45/06 , A61K2300/00
摘要： Compositions comprising coenzyme Q and ID02 inhibitors and methods of use thereof are provided.
摘要翻译：提供了包含辅酶Q和ID02抑制剂的组合物及其使用方法。

9. 发明申请

WO2008143668A2 INDOLEAMINE-2, 3-DIOXYGENASE-2 审中-公开
标题翻译： INDOLEAMINE-2，3-DIOXYGENASE-2
公开(公告)号：WO2008143668A2
公开(公告)日：2008-11-27
申请号：PCT/US2007/069271
申请日：2007-05-18
申请人： LANKENAU INSTITUTE FOR MEDICAL RESEARCH , PRENDERGAST, George, C. , METZ, Richard
发明人： PRENDERGAST, George, C. , METZ, Richard
IPC分类号： A01K67/027
CPC分类号： C12N9/0069 , A01K67/0276 , C07K2319/21 , C07K2319/40 , C12N2830/006 , C12Q1/26
摘要： The nucleotide and amino acid sequences of indoleamine 2, 3-dioxygenase-2 (ID02) and methods of use thereof are provided.
摘要翻译：提供了吲哚胺2,3-双加氧酶-2（ID02）的核苷酸和氨基酸序列及其使用方法。

10. 发明申请

WO03089609A2 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 审中-公开
标题翻译：短片代替同种异体替代抗性抗性
公开(公告)号：WO03089609A2
公开(公告)日：2003-10-30
申请号：PCT/US0312093
申请日：2003-04-17
申请人： METZ RICHARD , BLAESE MICHAEL R
发明人： METZ RICHARD , BLAESE MICHAEL R
IPC分类号： A01K67/027 , A61K48/00 , C12N20060101 , C12N15/09 , C12N15/85 , C12N15/877 , C12Q1/68 , C12N
CPC分类号： A01K67/0275 , A01K2227/101 , A01K2267/02 , C12N15/8509 , C12N15/8771 , C12N2790/10011 , C12N2790/10022
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alteration in the PrP gene is disclosed. The PrP gene of a cultured cell is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.
摘要翻译：公开了通过PrP基因的靶向改变产生对牛海绵状脑病耐牛的方法。改变培养的细胞的PrP基因以防止其翻译或编码蛋白质的显性疾病抗性形式，并且改变的细胞的细胞核被用于克隆创始者动物。在一个实施方案中，含有该改变的单链DNA片段用于单链短片段同源置换以改变PrP基因。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式