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1. 发明公开

EP1501351A2 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 审中-公开
标题翻译：同源短片段替代GENERATING疯牛病牛耐
公开(公告)号：EP1501351A2
公开(公告)日：2005-02-02
申请号：EP03741764.9
申请日：2003-04-17
申请人： Metz, Richard , Blaese, Michael R.
发明人： Metz, Richard , Blaese, Michael R.
IPC分类号： A01K67/027
CPC分类号： A01K67/0275 , A01K2227/101 , A01K2267/02 , C12N15/8509 , C12N15/8771 , C12N2790/10011 , C12N2790/10022
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alteration in the PrP gene is disclosed. The PrP gene of a cultured cell is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.

2. 发明专利

AU2003291073A8 A method for cloning of a rare, specifically mutated cell 未知
公开(公告)号：AU2003291073A8
公开(公告)日：2009-07-30
申请号：AU2003291073
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： BLAESE MICHAEL R , DICOLA MIKE , METZ RICHARD
IPC分类号： C12Q1/68 , C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/82 , C12N15/85

3. 发明专利

AU2003291073A1 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 未知
公开(公告)号：AU2003291073A1
公开(公告)日：2004-06-15
申请号：AU2003291073
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
IPC分类号： C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/85 , C12Q1/68

4. 发明申请

WO2004046335A3 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 审中-公开
标题翻译：用于克隆稀有特异性突变细胞的方法
公开(公告)号：WO2004046335A3
公开(公告)日：2009-06-18
申请号：PCT/US0336882
申请日：2003-11-18
申请人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
发明人： METZ RICHARD , DICOLA MIKE , BLAESE MICHAEL R
IPC分类号： C12Q1/68 , C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/82 , C12N15/85
CPC分类号： C12N15/1034
摘要： The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration. The method comprises sequentially using polymerase chain reaction (PCR) using an allele specific primer to preferentially amplify sequences containing one of the two linked alterations, coupled with a second technique that detects the second change in the PCR product. The second method may comprise restriction digestion, conventional DNA sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected using the methods provided.
摘要翻译：本发明涉及一种检测培养细胞的定向遗传改变的稀有产物的新方法。该方法适用于任何改变方法。该方法包括使用等位基因特异性引物依次使用聚合酶链反应（PCR）来优先扩增含有两个连接的改变之一的序列，加上检测PCR产物的第二个变化的第二个技术。第二种方法可以包括限制性消化，常规DNA测序或热解测序。实验表明，使用所提供的方法可以检测到10,000个细胞中一个正确改变的拷贝稀少的改变。

5. 发明申请

WO1995009655A1 TREATMENT OF TUMORS BY GENETIC TRANSFORMATION OF TUMOR CELLS WITH GENES ENCODING NEGATIVE SELECTIVE MARKERS AND CYTOKINES 审中-公开
标题翻译：通过基因编码阴性选择标记和细胞因子的肿瘤细胞的遗传转化来治疗肿瘤
公开(公告)号：WO1995009655A1
公开(公告)日：1995-04-13
申请号：PCT/US1994011251
申请日：1994-10-04
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES , CULVER, Kenneth, W. , LINK, Charles, J., Jr. , BLAESE, Michael, R.
发明人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES
IPC分类号： A61K48/00
CPC分类号： A61K48/00 , A61K38/00 , A61K39/0011 , C07K14/55
摘要： A method of treating a tumor in a host which comprises administering to the host at least one expression vehicle including a first nucleic acid sequence encoding an agent which renders the tumor cells sensitive to an interaction agent, and a second nucleic acid sequence which encodes an agent which provides for the inhibition, prevention, or destruction of the growth of the tumor cells, wherein such agent is not an agent which renders tumor cells sensitive to an interaction agent. The tumor then is treated with the interaction agent. Preferably, the first nucleic acid sequence encodes a negative selective marker (such as Herpes Simplex Virus thymidine kinase), and the second nucleic acid sequence encodes a cytokine (such as Interleukin-2), and the first and second nucleic acid sequences are contained in first and second viral vectors, each of which is contained in a first producer cell line and in a second producer cell line, respectively. The producer cell lines are administered to the tumor, whereby viral particles generated by the producer cell lines infect the tumor cells. Upon administration of an interaction agent, such as ganciclovir, to the host, the tumor cells are killed.
摘要翻译：一种治疗宿主中的肿瘤的方法，其包括对所述宿主施用至少一种表达载体，所述表达载体包含编码使所述肿瘤细胞对相互作用剂敏感的试剂的第一核酸序列和编码所述试剂的第二核酸序列其提供抑制，预防或破坏肿瘤细胞的生长，其中所述药剂不是使肿瘤细胞对相互作用剂敏感的药剂。然后用相互作用剂处理肿瘤。优选地，第一核酸序列编码阴性选择性标记（例如单纯疱疹病毒胸苷激酶），第二核酸序列编码细胞因子（例如白细胞介素-2），并且第一和第二核酸序列包含在第一和第二病毒载体，其各自分别包含在第一生产细胞系和第二生产细胞系中。向肿瘤施用生产细胞系，由生产细胞系产生的病毒颗粒感染肿瘤细胞。在向宿主施用相互作用剂如更昔洛韦后，将肿瘤细胞杀死。

6. 发明申请

WO03089609A2 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 审中-公开
标题翻译：短片代替同种异体替代抗性抗性
公开(公告)号：WO03089609A2
公开(公告)日：2003-10-30
申请号：PCT/US0312093
申请日：2003-04-17
申请人： METZ RICHARD , BLAESE MICHAEL R
发明人： METZ RICHARD , BLAESE MICHAEL R
IPC分类号： A01K67/027 , A61K48/00 , C12N20060101 , C12N15/09 , C12N15/85 , C12N15/877 , C12Q1/68 , C12N
CPC分类号： A01K67/0275 , A01K2227/101 , A01K2267/02 , C12N15/8509 , C12N15/8771 , C12N2790/10011 , C12N2790/10022
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alteration in the PrP gene is disclosed. The PrP gene of a cultured cell is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.
摘要翻译：公开了通过PrP基因的靶向改变产生对牛海绵状脑病耐牛的方法。改变培养的细胞的PrP基因以防止其翻译或编码蛋白质的显性疾病抗性形式，并且改变的细胞的细胞核被用于克隆创始者动物。在一个实施方案中，含有该改变的单链DNA片段用于单链短片段同源置换以改变PrP基因。

7. 发明申请

WO2004046335A2 A METHOD FOR CLONING OF A RARE, SPECIFICALLY MUTATED CELL 审中-公开
标题翻译：用于克隆稀有特异性突变细胞的方法
公开(公告)号：WO2004046335A2
公开(公告)日：2004-06-03
申请号：PCT/US2003/036882
申请日：2003-11-18
申请人： METZ, Richard , DICOLA, Mike , BLAESE, Michael, R.
发明人： METZ, Richard , DICOLA, Mike , BLAESE, Michael, R.
IPC分类号： C12N
CPC分类号： C12N15/1034
摘要： The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration. The method comprises sequentially using polymerase chain reaction (PCR) using an allele specific primer to preferentially amplify sequences containing one of the two linked alterations, coupled with a second technique that detects the second change in the PCR product. The second method may comprise restriction digestion, conventional DNA sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected using the methods provided.
摘要翻译：本发明涉及一种检测培养细胞的定向遗传改变的稀有产物的新方法。该方法适用于任何改变方法。该方法包括使用等位基因特异性引物依次使用聚合酶链反应（PCR）来优先扩增含有两个连接的改变之一的序列，加上检测PCR产物的第二个变化的第二个技术。第二种方法可以包括限制性消化，常规DNA测序或热解测序。实验表明，使用所提供的方法可以检测到10,000个细胞中一个正确改变的拷贝稀少的改变。

8. 发明申请

WO2003089609A3 SHORT FRAGMENT HOMOLOGOUS REPLACEMENT TO PROVIDE BSE RESISTANT CATTLE 审中-公开
公开(公告)号：WO2003089609A3
公开(公告)日：2003-10-30
申请号：PCT/US2003/012093
申请日：2003-04-17
申请人： METZ, Richard , BLAESE, Michael, R.
发明人： METZ, Richard , BLAESE, Michael, R.
IPC分类号： A01K67/027
摘要： A method for generating cattle resistant to Bovine Spongioform Encephalopathy through targeted alteration in the PrP gene is disclosed. The PrP gene of a cultured cell is altered to prevent its translation or to encode a dominant disease-resistant form of the protein, and the nucleus of the altered cell is used to clone a founder animal. In one embodiment, a single-stranded DNA fragment containing the alteration is used in single-stranded short fragment homologous replacement to alter the PrP gene.

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