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1. 发明授权

US06518066B1 Control of gene expression in eukaryotic cells 有权
公开(公告)号：US06518066B1
公开(公告)日：2003-02-11
申请号：US09608958
申请日：2000-06-30
申请人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
发明人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
IPC分类号： C12N1582
CPC分类号： C07D307/33 , A61K48/00 , C12N15/81 , C12N15/8214 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/30 , C12N2830/36 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.

2. 发明授权

US06403865B1 Method of producing transgenic maize using direct transformation of commercially important genotypes 失效
标题翻译：使用商业上重要的基因型的直接转化生产转基因玉米的方法
公开(公告)号：US06403865B1
公开(公告)日：2002-06-11
申请号：US08438666
申请日：1995-05-10
申请人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie , Nadine Carozzi , Annick De Framond , James O. Linder , Robert L. Miller , Bruce W. Skillings , Alan W. Mousel , Albert R. Hornbrook , Christopher P. Clucas , Moez Rajabali Meghji , Andreas H. Tanner , Francis E. Cassagne , Gilles Pollini , Terry Ray Colbert , Francis P. Cammack
发明人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie , Nadine Carozzi , Annick De Framond , James O. Linder , Robert L. Miller , Bruce W. Skillings , Alan W. Mousel , Albert R. Hornbrook , Christopher P. Clucas , Moez Rajabali Meghji , Andreas H. Tanner , Francis E. Cassagne , Gilles Pollini , Terry Ray Colbert , Francis P. Cammack
IPC分类号： A01H500
CPC分类号： C12N9/12 , C07K14/325 , C12N9/88 , C12N15/8286 , Y02A40/162
摘要： Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.
摘要翻译：公开了用感兴趣的核酸序列转化玉米的方法。该方法包括使未成熟的合子胚或I型愈伤组织进行高速微粒轰击。该方法能够生产具有商业重要性的转化玉米品系及其杂交组合。

3. 发明授权

US5824542A Methods for the production of hybrid seed 失效
公开(公告)号：US5824542A
公开(公告)日：1998-10-20
申请号：US449514
申请日：1995-05-23
申请人： Lyle D. Crossland , Annmarie Tuttle , Jeffrey I. Stein
发明人： Lyle D. Crossland , Annmarie Tuttle , Jeffrey I. Stein
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , C07K14/415 , C12N5/10 , C12N15/29 , C12N15/82 , C12N15/31
CPC分类号： C12N15/8216 , A01H1/00 , A01H1/02 , C07K14/415 , C12N15/8217 , C12N15/8289 , Y10S47/01
摘要： The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

4. 发明授权

US06943243B2 Control of gene expression in eukaryotic cells 有权
标题翻译：控制真核细胞中的基因表达
公开(公告)号：US06943243B2
公开(公告)日：2005-09-13
申请号：US10360123
申请日：2003-02-06
申请人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
发明人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
IPC分类号： A01H5/00 , A01K67/027 , A61K31/7088 , A61K48/00 , A61P31/00 , C07D307/33 , C07H21/04 , C12N1/19 , C12N5/04 , C12N5/10 , C12N9/18 , C12N15/09 , C12N15/81 , C12N15/82 , C12N15/85 , C12Q1/02 , C12Q1/68 , C12N5/14
CPC分类号： C07D307/33 , A61K48/00 , C12N15/81 , C12N15/8214 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/30 , C12N2830/36 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.
摘要翻译：用于控制真核细胞和生物体中的基因表达的DNA构建体和其它组合物和方法源自细菌群体感测系统。来自luxI启动子的一种或多种顺式元件（“lux框”）或功能上相似的序列并入真核启动子中。来自转录调节物的LuxR家族的受体蛋白质在结合酰化的高丝氨酸内酯（AHL）化合物时，与lux框相互作用，调节启动子的活性。

5. 发明授权

US5859336A Synthetic DNA sequence having enhanced activity in maize 失效
标题翻译：在玉米中具有增强活性的合成DNA序列
公开(公告)号：US5859336A
公开(公告)日：1999-01-12
申请号：US459448
申请日：1995-06-02
申请人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie
发明人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie
IPC分类号： A01H5/00 , A01H5/10 , C07H21/04 , C07K14/325 , C12N5/04 , C12N9/12 , C12N9/88 , C12N15/09 , C12N15/11 , C12N15/32 , C12N15/54 , C12N15/60 , C12N15/82 , C12N15/87 , C12N15/63
CPC分类号： C07K14/325 , C12N15/8286 , C12N9/12 , C12N9/88
摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.
摘要翻译：公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。还提供植物启动子，特定组织特异性和组织优选启动子。另外公开了包含所述DNA序列的转化载体。转化载体在转化为玉米时表现出高水平的杀虫活性。

6. 发明授权

US5659124A Transgenic male sterile plants for the production of hybrid seeds 失效
标题翻译：用于生产杂交种子的转基因雄性不育植物
公开(公告)号：US5659124A
公开(公告)日：1997-08-19
申请号：US368773
申请日：1995-01-03
申请人： Lyle D. Crossland , Annmarie Tuttle , Jeffrey I. Stein
发明人： Lyle D. Crossland , Annmarie Tuttle , Jeffrey I. Stein
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , C07K14/415 , C12N5/10 , C12N15/29 , C12N15/82 , C12N15/33
CPC分类号： C12N15/8216 , A01H1/00 , A01H1/02 , C07K14/415 , C12N15/8217 , C12N15/8289 , Y10S47/01
摘要： The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed.The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.
摘要翻译：本发明提供了用于产生雄性不育植物的双重方法。两个遗传转化的植物，父母1和2交叉获得雄性不育后代。用表达盒转化亲本1，所述表达盒包含编码花药特异性启动子的核苷酸序列，其与编码反式激活因子的核苷酸序列可操作地连接。用包含目标核苷酸序列的表达盒转化亲本2，所述靶核苷酸序列能够由反式激活因子活化，可操作地连接到编码RNA或多肽的核苷酸序列，所述核苷酸序列将破坏活的花粉的形成。因此，父母1与父母2交叉导致雄性不育后代。雄性不育植物可用于产生杂交种子。本发明还提供用于植物中感兴趣的编码区高水平表达的组合物和方法。

7. 发明授权

US6147282A Method of controlling the fertility of a plant 有权
标题翻译：控制植物生育力的方法
公开(公告)号：US6147282A
公开(公告)日：2000-11-14
申请号：US234190
申请日：1999-01-20
申请人： Stephen A. Goff , Lyle D. Crossland , Laura S. Privalle
发明人： Stephen A. Goff , Lyle D. Crossland , Laura S. Privalle
IPC分类号： A01H5/00 , C07K14/705 , C07K14/72 , C12N5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/82 , C12P21/02 , C12R1/91 , A01H1/02 , C12N15/55
CPC分类号： C12N15/8216 , C07K14/70567 , C07K14/721 , C12N15/8217 , C12N15/8237 , C12N15/8289 , C12N15/829 , C07K2319/00
摘要： The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises obtaining a transgenic plant comprising at least two receptor expression cassettes and at least one target expression cassette. The first receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a first receptor polypeptide, and a 3' termination region. The second receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a second receptor polypeptide, and a 3' termination region. The target expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a target polypeptide, and a 3' termination region, wherein the 5' regulatory region of said target expression cassette is activated by said first and second receptor polypeptides in the presence of one or more chemical ligands which are complementary to the ligand binding domain of said receptor polypeptides, whereby expression of said target polypeptide is accomplished. The method is useful for controlling various traits of agronomic importance, such as plant fertility.
摘要翻译：本发明涉及控制植物中基因表达的方法。具体地，该方法包括获得包含至少两个受体表达盒和至少一个靶标表达盒的转基因植物。第一受体表达盒包含与编码第一受体多肽的核苷酸序列和3'终止区可操作地连接的5'调控区的核苷酸序列。第二受体表达盒包含与编码第二受体多肽的核苷酸序列可操作地连接的5'调控区的核苷酸序列和3'终止区。靶表达盒包含与编码靶多肽的核苷酸序列有效连接的5'调控区的核苷酸序列和3'终止区，其中所述靶表达盒的5'调节区被所述第一和第第二受体多肽在与所述受体多肽的配体结合结构域互补的一个或多个化学配体存在下进行，从而实现所述靶多肽的表达。该方法可用于控制农艺学重要性的各种性状，如植物生育力。

8. 发明授权

US5880333A Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand 失效
标题翻译：通过受体介导的反式激活在化学配体的存在下控制植物中的基因表达
公开(公告)号：US5880333A
公开(公告)日：1999-03-09
申请号：US10050
申请日：1998-01-21
申请人： Stephen A. Goff , Lyle D. Crossland , Laura S. Privalle
发明人： Stephen A. Goff , Lyle D. Crossland , Laura S. Privalle
IPC分类号： A01H5/00 , C07K14/705 , C07K14/72 , C12N5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/82 , C12P21/02 , C12R1/91
CPC分类号： C12N15/8216 , C07K14/70567 , C07K14/721 , C12N15/8217 , C12N15/8237 , C12N15/8289 , C12N15/829 , C07K2319/00
摘要： The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises obtaining a transgenic plant comprising at least two receptor expression cassettes and at least one target expression cassette. The first receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a first receptor polypeptide, and a 3' termination region. The second receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a second receptor polypeptide, and a 3' termination region. The target expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a target polypeptide, and a 3' termination region, wherein the 5' regulatory region of said target expression cassette is activated by said first and second receptor polypeptides in the presence of one or more chemical ligands which are complementary to the ligand binding domain of said receptor polypeptides, whereby expression of said target polypeptide is accomplished. The method is useful for controlling various traits of agronomic importance, such as plant fertility.
摘要翻译：本发明涉及控制植物中基因表达的方法。具体地，该方法包括获得包含至少两个受体表达盒和至少一个靶标表达盒的转基因植物。第一受体表达盒包含与编码第一受体多肽的核苷酸序列和3'终止区可操作地连接的5'调控区的核苷酸序列。第二受体表达盒包含与编码第二受体多肽的核苷酸序列可操作地连接的5'调控区的核苷酸序列和3'终止区。靶表达盒包含与编码靶多肽的核苷酸序列有效连接的5'调控区的核苷酸序列和3'终止区，其中所述靶表达盒的5'调节区被所述第一和第第二受体多肽在与所述受体多肽的配体结合结构域互补的一个或多个化学配体存在下进行，从而实现所述靶多肽的表达。该方法可用于控制农艺学重要性的各种性状，如植物生育力。

9. 发明授权

US5477002A Anther-specific CDNA sequences, genomic DNA sequences and recumbinant DNA sequences 失效
标题翻译：花药特异性CDNA序列，基因组DNA序列和重叠DNA序列
公开(公告)号：US5477002A
公开(公告)日：1995-12-19
申请号：US207904
申请日：1994-03-07
申请人： Annmarie B. Tuttle , Lyle D. Crossland
发明人： Annmarie B. Tuttle , Lyle D. Crossland
IPC分类号： C07K14/415 , C12N1/21 , C12N15/82 , A01H5/00 , C12N15/11
CPC分类号： C07K14/415 , C12N15/8289
摘要： cDNA sequences are disclosed which are expressed specifically in the anther of a plant. Genomic DNA sequences corresponding to the cDNA clones are obtained using the cDNA clones as hybridization probes. Recombinant, or chimeric, DNA sequences are constructed in which the promoter sequence from anther-specific genomic clones are operatively linked to a DNA sequence coding for a desired polypeptide. Transgenic plants are made in which the chimeric DNA sequences are expressed in the anther of the transgenic plant. In a preferred embodiment, the coding DNA sequence expresses a polypeptide which will disrupt formation of viable pollen, resulting in a male-sterile plant.
摘要翻译：公开了在植物花药中特异性表达的cDNA序列。使用cDNA克隆作为杂交探针获得对应于cDNA克隆的基因组DNA序列。构建重组或嵌合的DNA序列，其中来自花药特异性基因组克隆的启动子序列可操作地连接到编码所需多肽的DNA序列。制备转基因植物，其中嵌合DNA序列在转基因植物的花药中表达。在优选的实施方案中，编码DNA序列表达将破坏活花粉形成的多肽，导致雄性不育植物。

10. 发明授权

US07202085B2 Control of gene expression in eukaryotic cells 有权
标题翻译：控制真核细胞中的基因表达
公开(公告)号：US07202085B2
公开(公告)日：2007-04-10
申请号：US11145451
申请日：2005-06-03
申请人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
发明人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
IPC分类号： C12N5/14 , C12N5/10 , A01H5/00
CPC分类号： C07D307/33 , A61K48/00 , C12N15/81 , C12N15/8214 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/30 , C12N2830/36 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.
摘要翻译：用于控制真核细胞和生物体中的基因表达的DNA构建体和其它组合物和方法源自细菌群体感测系统。来自luxI启动子的一种或多种顺式元件（“lux框”）或功能上相似的序列并入真核启动子中。来自转录调节物的LuxR家族的受体蛋白质在结合酰化的高丝氨酸内酯（AHL）化合物时，与lux框相互作用，调节启动子的活性。

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