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    • 4. 发明授权
    • Method of inhibiting viral replication in eukaryotic cells and of
inducing apoptosis of virally-infected cells
    • 抑制真核细胞中病毒复制和诱导病毒感染细胞凋亡的方法
    • US5849587A
    • 1998-12-15
    • US488811
    • 1995-06-09
    • Hartmut M. Hanauske-AbelRobert Walter GradyAxel HanauskeLinda AndrusPaul Szabo
    • Hartmut M. Hanauske-AbelRobert Walter GradyAxel HanauskeLinda AndrusPaul Szabo
    • A61K45/00A61K31/00A61K31/44A61K31/4412A61K38/04A61K38/06A61P31/00A61P31/12C12N5/08
    • A61K38/06A61K31/00A61K31/4412A61K38/04
    • The present invention is directed to methods which employ inhibition of the post-translational hypusine formation in the intracelluar protein eIF-5A, for the purpose of suppressing infections by viruses that parasitize eIF-5A so as to promote their own replication. Intentional inhibition of the post-translational formation of hypusine in infected host cells with compounds generically termed `hypusine inhibitors` not only selectively suppresses the production of viral proteins and of infectious viral particles, but also causes, particularly after hypusine inhibitor withdrawal, apoptosis in such virally-infected cells. Each of these methods, respectively, involves administering, to eukaryotic cells, tissues, or individuals, an agent which blocks the post-translational intracellular formation of hypusine, in an amount sufficient to: suppress biosynthesis of bioactive eIF-5A, suppress translational interaction of eIF-5A with viral elements of nucleic acid and/or protein structure, inhibit biosynthesis of viral proteins of Rev-dependent lentiviruses or of viruses dependent on interaction of eIF-5A with viral elements of nucleic acid and/or protein structure, inhibit replication of Rev-dependent lentiviruses or of viruses dependent on interaction of eIF-5A with viral elements of nucleic acid and/or protein structure, and induce apoptosis of virally-infected cells. This agent can be a compound of Formulae I or II and derivatives thereof as follows: ##STR1## R.sub.1, R.sub.2, R.sub.3, and R.sub.4 each individually represent a hydrogen, an alkyl, alkenyl, or alkoxy group containing 1 to about 8 carbon atoms, an aryl, aralkyl, or cycloalkyl group containing about 5 to 12 carbon atoms, or a carboalkoxy or carbamyl group containing up to 8 carbon atoms, or a peptide or peptidomimetic moiety containing 10 to about 30 carbon atoms.
    • 本发明涉及在胞内蛋白eIF-5A中采用抑制翻译后丘脑形成抑制作用的方法,目的是抑制寄生eIF-5A的病毒感染以促进其自身的复制。 通常称为“hypusine抑制剂”的化合物在感染宿主细胞中翻译后形成hypusine的有意抑制不仅选择性地抑制病毒蛋白质和感染性病毒颗粒的产生,而且还导致,特别是在hypusine抑制剂撤除后,细胞凋亡 病毒感染细胞。 这些方法中的每一种分别涉及向真核细胞,组织或个体施用足以抑制生物活性eIF-5A的生物合成的量的阻断翻译后细胞内形成hypusine的药剂,抑制翻译后的相互作用 具有核酸和/或蛋白质结构的病毒元件的eIF-5A抑制Rev依赖性慢病毒的病毒蛋白的生物合成或依赖于eIF-5A与核酸和/或蛋白质结构的病毒元件相互作用的病毒,抑制复制 依赖于依赖于依赖于依赖于eIF-5A与核酸和/或蛋白质结构的病毒元件的相互作用的依赖于慢病毒的病毒或依赖于病毒感染细胞凋亡的病毒。 该试剂可以是式I或II的化合物及其衍生物如下:R1,R2,R3和R4各自独立地表示氢,烷基,烯基或烷氧基,其含有1〜 约8个碳原子,含有约5至12个碳原子的芳基,芳烷基或环烷基,或含有至多8个碳原子的烷氧基或氨基甲酰基,或含有10至约30个碳原子的肽或肽模拟部分。