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1. 发明申请

US20120216320A1 METHOD FOR MODULATING PLANT GROWTH, NUCLEIC ACID MOLECULES AND POLYPEPTIDES ENCODED THEREOF USEFUL AS MODULATING AGENT 审中-公开
标题翻译：调节植物生长的方法，核酸分子和编码的多糖作为调节剂
公开(公告)号：US20120216320A1
公开(公告)日：2012-08-23
申请号：US13465078
申请日：2012-05-07
申请人： Dirk Inze , Veronique Boudolf , Lieven de Veylder , Juan Antonio Torres Acosta , Zoltan Magyar
发明人： Dirk Inze , Veronique Boudolf , Lieven de Veylder , Juan Antonio Torres Acosta , Zoltan Magyar
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N1/19 , C12P21/00 , G01N33/566 , C12Q1/68 , C12Q1/48 , C12N15/11 , C07K14/00 , C07K16/00 , A01N37/44 , A01N57/24 , A01P21/00 , C12N15/63
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The invention provides isolated nucleic acids molecules, designated CCP nucleic acid molecules, which encode novel cell cycle associated polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing CCP nucleic acid molecules, host cells into which the expression vectors have been introduced, and transgenic plants in which a CCP gene has been introduced or disrupted. The invention still further provides isolated CCP proteins, fusion proteins, antigenic peptides and anti-CCP antibodies. Agricultural, diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
摘要翻译：本发明提供编码新的细胞周期相关多肽的分离的核酸分子，命名为CCP核酸分子。本发明还提供了反义核酸分子，含有CCP核酸分子的重组表达载体，其中引入了表达载体的宿主细胞，以及CCP基因被引入或破坏的转基因植物。本发明还进一步提供了分离的CCP蛋白，融合蛋白，抗原肽和抗CCP抗体。还提供了利用本发明组合物的农业，诊断，筛选和治疗方法。

2. 发明申请

US20110162107A1 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND METHODS FOR MAKING THE SAME 审中-公开
标题翻译：具有改善生长特性的植物及其制备方法
公开(公告)号：US20110162107A1
公开(公告)日：2011-06-30
申请号：US12943243
申请日：2010-11-10
申请人： Dirk INZE , Lieven De Veylder , Kobe Vlieghe
发明人： Dirk INZE , Lieven De Veylder , Kobe Vlieghe
IPC分类号： A01H5/00 , C12N5/10 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention concerns a method for altering characteristics of a plant. The invention describes the identification of a gene that is downregulated in transgenic plants overexpressing E2Fa/DPa and the use of such sequences to alter plant characteristics. A preferred way for altering characteristics of a plant comprises modifying expression of one or more nucleic acid sequences and or modifying level and/or activity of one or more proteins, which nucleic acids and/or proteins encoded thereby are essentially similar to SEQ ID NO 1835. The gene identified in the present invention have an E2Fa target consensus sequence in their 5′ upstream region. The identified gene is postulated to play a role as transcription factors.
摘要翻译：本发明涉及改变植物特性的方法。本发明描述了在过表达E2Fa / DPa的转基因植物中下调的基因的鉴定以及使用这种序列来改变植物特征。用于改变植物特征的优选方法包括修饰一个或多个核酸序列的表达和/或修饰一种或多种蛋白质的水平和/或活性，其中由此编码的核酸和/或蛋白质基本上类似于SEQ ID NO 1835 本发明鉴定的基因在其5'上游区具有E2Fa靶共有序列。假定鉴定的基因作为转录因子发挥作用。

3. 发明授权

US07265267B1 Cyclin-dependent kinase inhibitors and uses thereof 失效
标题翻译：细胞周期蛋白依赖性激酶抑制剂及其用途
公开(公告)号：US07265267B1
公开(公告)日：2007-09-04
申请号：US09574735
申请日：2000-05-18
申请人： Lieven De Veylder , Tom Beeckman , Dirk Inze , Wim Van Camp , Luc Krols
发明人： Lieven De Veylder , Tom Beeckman , Dirk Inze , Wim Van Camp , Luc Krols
IPC分类号： C12N15/82 , A01H5/00 , A01H5/02
CPC分类号： C07K14/415 , A01N65/08 , C12N15/8261 , Y02A40/146
摘要： Provided are DNA sequences encoding cyclin-dependent kinase inhibitor(s) as well as to methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences which naturally regulate the expression of the above described DNA sequences are described. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cyclin-dependent kinase inhibitor(s) functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided is a process for disruption plant cell division by interfering in the expression or activity of a cyclin-dependent protein kinase inhibitor using a DNA sequence according to the invention wherein said plant cell is part of a transgenic plant. Further described are diagnostic compositions comprising the aforementioned DNA sequences, proteins, antibodies and regulatory sequences. Methods for the identification of compounds being capable of activating or inhibiting the cyclin-dependent kinase inhibitors are described as well. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
摘要翻译：提供编码细胞周期蛋白依赖性激酶抑制剂的DNA序列以及获得其的方法。此外，描述了包含所述DNA序列的载体，其中所述DNA序列可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。此外，提供了由所述DNA序列编码的蛋白质，对所述蛋白质的抗体及其生产方法。此外，描述了天然调节上述DNA序列表达的调节序列。还描述了一种用于控制或改变植物和/或植物细胞的生长特征的方法，包括在植物或其部分和/或其中的一个或多个部分引入和/或表达功能性的一种或多种细胞周期蛋白依赖性激酶抑制剂和/ 更多编码这种蛋白质的DNA序列。还提供了通过使用根据本发明的DNA序列干扰细胞周期蛋白依赖性蛋白激酶抑制剂的表达或活性来破坏植物细胞分裂的方法，其中所述植物细胞是转基因植物的一部分。进一步描述了包含上述DNA序列，蛋白质，抗体和调控序列的诊断组合物。还描述了能够激活或抑制细胞周期蛋白依赖性激酶抑制剂的化合物的鉴定方法。此外，还描述了含有上述DNA序列和载体的转基因植物细胞，植物组织和植物以及上述DNA序列，载体，蛋白质，抗体，调节序列和/或化合物在植物细胞和组织培养，植物育种和/或农业中的发明。

4. 发明授权

US08853492B2 Plants having improved growth characteristics and a method for making the same 有权
标题翻译：具有改善的生长特性的植物及其制备方法
公开(公告)号：US08853492B2
公开(公告)日：2014-10-07
申请号：US11799083
申请日：2007-04-30
申请人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
发明人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
IPC分类号： A01H5/00 , A01H1/00 , C12N15/09 , C12N15/82 , C07K14/415 , C12N9/12
CPC分类号： C07K14/415 , C12N9/1205 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及相对于相应野生型植物改善植物生长特性的方法。更具体地，本发明涉及一种改善植物生长特性的方法，包括调节植物中编码I型同源域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的表达; 或包括调节植物中编码硝酸转运蛋白（NRT）或其同源物的核酸的表达; 或包括调节植物中编码称为产量增强蛋白16（YEP16）的多肽的核酸的表达; 或包括调节I型糖原合酶激酶（I类绒毛样激酶）植物中的表达或其同源物。本发明还涉及具有调节I型HDZip hox5多肽或其同源物的核酸表达的植物; 或已调节编码NRT蛋白或其同源物的核酸的表达; 或已调节编码表示为YEP16的多肽的核酸的表达; 或具有调节I组粗毛状激酶或其同源物的表达，该植物相对于相应的野生型植物具有改善的生长特性。本发明还提供了可用于本发明方法的构建体。

5. 发明授权

US07592507B2 Method to modify cell number, architecture and yield of plants by overexpressing the E2F transcription factor 失效
标题翻译：通过过表达E2F转录因子修饰植物的细胞数量，结构和产量的方法
公开(公告)号：US07592507B2
公开(公告)日：2009-09-22
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

6. 发明申请

US20080127365A1 Plants having improved growth characteristics and a method for making the same 有权
标题翻译：具有改善的生长特性的植物及其制备方法
公开(公告)号：US20080127365A1
公开(公告)日：2008-05-29
申请号：US11799083
申请日：2007-04-30
申请人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
发明人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
IPC分类号： C12N15/87 , A01H1/00 , C12N5/04 , C12Q1/68 , A01H5/00
CPC分类号： C07K14/415 , C12N9/1205 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及相对于相应野生型植物改善植物生长特性的方法。更具体地，本发明涉及一种改善植物生长特性的方法，包括调节植物中编码I型同源域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的表达; 或包括调节植物中编码硝酸转运蛋白（NRT）或其同源物的核酸的表达; 或包括调节植物中编码称为产量增强蛋白16（YEP16）的多肽的核酸的表达; 或包括调节I型糖原合酶激酶（I类绒毛样激酶）植物中的表达或其同源物。本发明还涉及具有调节I型HDZip hox5多肽或其同源物的核酸表达的植物; 或已调节编码NRT蛋白或其同源物的核酸的表达; 或已调节编码表示为YEP16的多肽的核酸的表达; 或具有调节I组粗毛状激酶或其同源物的表达，该植物相对于相应的野生型植物具有改善的生长特性。本发明还提供了可用于本发明方法的构建体。

7. 发明申请

US20090222952A1 Plants Having Improved Growth Characteristics And A Method For Making The Same 审中-公开
标题翻译：具有改善生长特性的植物及其制作方法
公开(公告)号：US20090222952A1
公开(公告)日：2009-09-03
申请号：US12092844
申请日：2006-11-08
申请人： Jan de Meutter , Vladimir Mironov , Christophe Reuzeau , Dirk Inze , Kobe Vlieghe , Lieven de Veylder
发明人： Jan de Meutter , Vladimir Mironov , Christophe Reuzeau , Dirk Inze , Kobe Vlieghe , Lieven de Veylder
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63 , C07K14/415 , C12Q1/68
CPC分类号： C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention concerns a method for increasing plant yield and/or increased stress resistance by decreasing the level of activity of a DEL1 polypeptide. One such method comprises introducing into a plant a nucleic acid comprising a variant DEL1 nucleic acid. Another method comprises downregulating expression of a DEL1 gene. The invention also relates to transgenic plants having introduced therein a variant DEL1 nucleic acid thereof, which plants have increased yield and/or increased stress resistance relative to control plants. The present invention also concerns a novel DEL1 protein and its encoding sequence, and constructs useful in the methods of the invention.
摘要翻译：本发明涉及通过降低DEL1多肽的活性水平来提高植物产量和/或增加的胁迫抗性的方法。一种这样的方法包括向植物中引入包含变体DEL1核酸的核酸。另一种方法包括下调DEL1基因的表达。本发明还涉及其中引入了其变体DEL1核酸的转基因植物，该植物相对于对照植物具有增加的产量和/或增加的胁迫抗性。本发明还涉及一种新颖的DEL1蛋白及其编码序列，以及可用于本发明方法的构建体。

8. 发明授权

US06710227B1 Cyclin-dependent kinase inhibitors and uses thereof 失效
公开(公告)号：US06710227B1
公开(公告)日：2004-03-23
申请号：US09526597
申请日：2000-03-16
申请人： Dirk Inze , Lieven De Veylder , Janice De Almeida , Isabelle Landrieu
发明人： Dirk Inze , Lieven De Veylder , Janice De Almeida , Isabelle Landrieu
IPC分类号： C12N1529
CPC分类号： C07K14/415 , A01N65/08 , C12N15/8261 , Y02A40/146
摘要： Provided are DNA sequences encoding cyclin-dependent kinase inhibitor(s) as well as to methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences which naturally regulate the expression of the above described DNA sequences are described. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cyclin-dependent kinase inhibitor(s) functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided is a process for disruption plant cell division by interfering in the expression or activity of a cyclin-dependent protein kinase inhibitor using a DNA sequence according to the invention wherein said plant cell is part of a transgenic plant. Further described are diagnostic compositions comprising the aforementioned DNA sequences, proteins, antibodies and regulatory sequences. Methods for the identification of compounds being capable of activating or inhibiting the cyclin-dependent kinase inhibitors are described as well. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.

9. 发明授权

US08193414B2 Method for modulating plant growth, nucleic acid molecules and polypeptides encoded thereof useful as modulating agent 失效
标题翻译：用于调节植物生长的方法，其编码的核酸分子和多肽可用作调节剂
公开(公告)号：US08193414B2
公开(公告)日：2012-06-05
申请号：US10276032
申请日：2001-05-14
申请人： Dirk Inze , Veronique Boudolf , Lieven De Veylder , Juan Antonio Torres Acosta , Zoltan Magyar
发明人： Dirk Inze , Veronique Boudolf , Lieven De Veylder , Juan Antonio Torres Acosta , Zoltan Magyar
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates to the field of molecular biology. The invention provides a method for modulating growth of a plant comprising introducing into a plant a CCP (cell cycle protein) modulator. Further provided are nucleic acid molecules and the polypeptides encoded thereof useful as modulating agents for performing such methods.
摘要翻译：本发明涉及分子生物学领域。本发明提供了调节植物生长的方法，包括向植物中引入CCP（细胞周期蛋白）调节剂。进一步提供的是核酸分子和其编码的多肽可用作用于进行这些方法的调节剂。

10. 发明授权

US07847156B2 Plants having improved growth characteristics and methods for making the same 有权
标题翻译：具有改善生长特性的植物及其制备方法
公开(公告)号：US07847156B2
公开(公告)日：2010-12-07
申请号：US10531475
申请日：2003-10-20
申请人： Dirk Inze , Lieven De Veylder , Kobe Vlieghe
发明人： Dirk Inze , Lieven De Veylder , Kobe Vlieghe
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention concerns a method for altering characteristics of a plant. The invention describes the identification of a gene that is downregulated in transgenic plants overexpressing E2Fa/DPa and the use of such sequences to alter plant characteristics. A preferred way for altering characteristics of a plant comprises modifying expression of one or more nucleic acid sequences and or modifying level and/or activity of one or more proteins, which nucleic acids and/or proteins encoded thereby are essentially similar to SEQ ID NO 1835. The gene identified in the present invention have an E2Fa target consensus sequence in their 5′ upstream region. The identified gene is postulated to play a role as transcription factors.
摘要翻译：本发明涉及改变植物特性的方法。本发明描述了在过表达E2Fa / DPa的转基因植物中下调的基因的鉴定以及使用这种序列来改变植物特征。用于改变植物特征的优选方法包括修饰一个或多个核酸序列的表达和/或修饰一种或多种蛋白质的水平和/或活性，其中由此编码的核酸和/或蛋白质基本上类似于SEQ ID NO 1835 本发明鉴定的基因在其5'上游区具有E2Fa靶共有序列。假定鉴定的基因作为转录因子发挥作用。

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