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    • 4. 发明申请
    • FLUIDIC DROPLET COALESCENCE
    • 流体滴灌技术
    • WO2007089541A3
    • 2007-11-15
    • PCT/US2007002063
    • 2007-01-24
    • HARVARD COLLEGEAHN KEUNHOCHONG HENRYAGRESTI JEREMYWEITZ DAVID ALINK DARREN ROY
    • AHN KEUNHOCHONG HENRYAGRESTI JEREMYWEITZ DAVID ALINK DARREN ROY
    • B01F13/00B01J19/00
    • B01F13/0071B01F13/0076B01J19/0093B01J2219/00272B01J2219/00828B01J2219/00833B01J2219/00835B01J2219/00853B01J2219/00889B01J2219/0093B01L3/5027B01L3/502784B01L2200/0673B01L2300/0867B01L2400/0415B01L2400/0487
    • The present invention generally relates to methods for the control of fluidic species and, in particular, to the coalescence of fluidic droplets. In certain instances, the methods are microf luidic. In one aspect, the invention relates to methods for causing two or more fluidic droplets within a channel to coalescence. The fluidic droplets may be of unequal size in certain cases. In some embodiments, a first fluidic droplet (21) may be caused to move at a first velocity, and a second fluidic droplet (22) may be caused to move at a second velocity different from the first velocity, for instance, substantially greater than the first velocity. The droplets may then coalesce, for example, upon application of an electric field. In the absence of an electric field, in some cases, the droplets may be unable to coalesce. In some cases, two series of fluidic droplets may coalesce, one or both series being substantially uniform. For instance, one series of droplets may have a distribution of diameters such that no more than about 5% of the droplets have a diameter greater than about 10% of the average diameter. In certain cases, one or more series of droplets may each consist essentially of a substantially uniform number of entities of a species therein (i.e., molecules, cells, particles, etc.). The fluidic droplets may be coalesced to start a reaction, and/or to stop a reaction, in some cases. For instance, a reaction may be initiated when a species in a first droplet contacts a species in a second droplet after the droplets coalesce, or a first droplet may contain an ongoing reaction and a second droplet may contain a species that inhibits the reaction.
    • 本发明一般涉及用于控制流体物种的方法,尤其涉及流体液滴聚结的方法。 在某些情况下,该方法是微流感。 一方面,本发明涉及使通道内的两个或多个流体液滴聚结的方法。 在某些情况下,流体液滴的尺寸可能不相等。 在一些实施例中,可以使第一流体液滴(21)以第一速度移动,并且可以使第二流体液滴(22)以不同于第一速度的第二速度移动,例如基本上大于 第一速度。 例如,当施加电场时,液滴可以聚结。 在没有电场的情况下,在一些情况下,液滴可能不能聚结。 在一些情况下,两系列流体液滴可以聚结,一个或两个串联基本均匀。 例如,一系列液滴可以具有直径分布,使得不超过约5%的液滴具有大于平均直径的约10%的直径。 在某些情况下,一个或多个液滴系列可以各自基本上由基本上一致数量的物质实体(即分子,细胞,颗粒等)组成。 在某些情况下,流体液滴可能被聚结起始反应,和/或停止反应。 例如,当液滴聚结之后,当第一液滴中的物质接触第二液滴中的物质时,或者第一液滴可能含有正在进行的反应,并且第二液滴可以含有抑制反应的物质时,反应可以开始。
    • 10. 发明申请
    • FORMATION AND CONTROL OF FLUIDIC SPECIES
    • 流体物种的形成与控制
    • WO2004091763A3
    • 2005-02-17
    • PCT/US2004010903
    • 2004-04-09
    • HARVARD COLLEGELINK DARREN RWEITZ DAVID AMARQUEZ-SANCHEZ MANUELCHENG ZHENGDONG
    • LINK DARREN RWEITZ DAVID AMARQUEZ-SANCHEZ MANUELCHENG ZHENGDONG
    • B01F5/06B01F13/00B01J2/04B05B5/03B05B7/06B05B5/025
    • B01L3/502784B01F5/0682B01F13/0062B01F13/0071B01F13/0076B01F2215/0431B01J2/04B01L3/502715B01L3/50273B01L2200/0673B01L2300/0867B01L2400/02B01L2400/0415B01L2400/0424B05B5/03B05B7/061
    • This invention generally relates to systems and methods for the formation and/or control of fluidic species, and articles produced by such systems and methods. In some cases, the invention involves unique fluid channels, systems, controls, and/or restrictions, and combinations thereof. In certain embodiments, the invention allows fluidic streams (which can be continuous or discontinuous, i.e., droplets) to be formed and/or combined, at a variety of scales, including microfluidic scales. In one set of embodiments, a fluidic stream may be produced from a channel, where a cross-sectional dimension of the fluidic stream is smaller than that of the channel, for example, through the use of structural elements, other fluids, and/or applied external fields, etc. In some cases, a Taylor cone may be produced. In another set of embodiments, a fluidic stream may be manipulated in some fashion, for example, to create tubes (which may be hollow or solid), droplets, nested tubes or droplets, arrays of tubes or droplets, meshes of tubes, etc. In some cases, droplets produced using certain embodiments of the invention may be charged or substantially charged, which may allow their further manipulation, for instance, using applied external fields. Non-limiting examples of such manipulations include producing charged droplets, coalescing droplets (especially at the microscale), synchronizing droplet formation, aligning molecules within the droplet, etc. In some cases, the droplets and/or the fluidic streams may include colloids, cells, therapeutic agents, and the like.
    • 本发明一般涉及用于形成和/或控制流体物质的系统和方法,以及由这些系统和方法生产的制品。 在一些情况下,本发明涉及独特的流体通道,系统,控制和/或限制及其组合。 在某些实施方案中,本发明允许流体流(其可以是连续的或不连续的,即液滴)以各种尺度形成和/或组合,包括微流体尺度。 在一组实施例中,流体流可以从通道产生,其中流体流的横截面尺寸小于通道的横截面尺寸,例如通过使用结构元件,其它流体和/或 应用外场等。在某些情况下,可能产生泰勒锥。 在另一组实施例中,流体流可以以某种方式操纵,例如,以产生管(其可以是中空或固体),液滴,嵌套管或液滴,管或液滴阵列,管的网格等。 在一些情况下,使用本发明的某些实施方案产生的液滴可能被充电或基本上带电,这可能允许其进一步操作,例如使用外部应用场。 这种操作的非限制性实例包括产生带电液滴,聚集液滴(特别是在微量级),同步液滴形成,使液滴中的分子对齐等。在一些情况下,液滴和/或流体流可包括胶体,细胞 ,治疗剂等。