专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007089541A3 FLUIDIC DROPLET COALESCENCE 审中-公开
标题翻译：流体滴灌技术
公开(公告)号：WO2007089541A3
公开(公告)日：2007-11-15
申请号：PCT/US2007002063
申请日：2007-01-24
申请人： HARVARD COLLEGE , AHN KEUNHO , CHONG HENRY , AGRESTI JEREMY , WEITZ DAVID A , LINK DARREN ROY
发明人： AHN KEUNHO , CHONG HENRY , AGRESTI JEREMY , WEITZ DAVID A , LINK DARREN ROY
IPC分类号： B01F13/00 , B01J19/00
CPC分类号： B01F13/0071 , B01F13/0076 , B01J19/0093 , B01J2219/00272 , B01J2219/00828 , B01J2219/00833 , B01J2219/00835 , B01J2219/00853 , B01J2219/00889 , B01J2219/0093 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487
摘要： The present invention generally relates to methods for the control of fluidic species and, in particular, to the coalescence of fluidic droplets. In certain instances, the methods are microf luidic. In one aspect, the invention relates to methods for causing two or more fluidic droplets within a channel to coalescence. The fluidic droplets may be of unequal size in certain cases. In some embodiments, a first fluidic droplet (21) may be caused to move at a first velocity, and a second fluidic droplet (22) may be caused to move at a second velocity different from the first velocity, for instance, substantially greater than the first velocity. The droplets may then coalesce, for example, upon application of an electric field. In the absence of an electric field, in some cases, the droplets may be unable to coalesce. In some cases, two series of fluidic droplets may coalesce, one or both series being substantially uniform. For instance, one series of droplets may have a distribution of diameters such that no more than about 5% of the droplets have a diameter greater than about 10% of the average diameter. In certain cases, one or more series of droplets may each consist essentially of a substantially uniform number of entities of a species therein (i.e., molecules, cells, particles, etc.). The fluidic droplets may be coalesced to start a reaction, and/or to stop a reaction, in some cases. For instance, a reaction may be initiated when a species in a first droplet contacts a species in a second droplet after the droplets coalesce, or a first droplet may contain an ongoing reaction and a second droplet may contain a species that inhibits the reaction.
摘要翻译：本发明一般涉及用于控制流体物种的方法，尤其涉及流体液滴聚结的方法。在某些情况下，该方法是微流感。一方面，本发明涉及使通道内的两个或多个流体液滴聚结的方法。在某些情况下，流体液滴的尺寸可能不相等。在一些实施例中，可以使第一流体液滴（21）以第一速度移动，并且可以使第二流体液滴（22）以不同于第一速度的第二速度移动，例如基本上大于第一速度。例如，当施加电场时，液滴可以聚结。在没有电场的情况下，在一些情况下，液滴可能不能聚结。在一些情况下，两系列流体液滴可以聚结，一个或两个串联基本均匀。例如，一系列液滴可以具有直径分布，使得不超过约5％的液滴具有大于平均直径的约10％的直径。在某些情况下，一个或多个液滴系列可以各自基本上由基本上一致数量的物质实体（即分子，细胞，颗粒等）组成。在某些情况下，流体液滴可能被聚结起始反应，和/或停止反应。例如，当液滴聚结之后，当第一液滴中的物质接触第二液滴中的物质时，或者第一液滴可能含有正在进行的反应，并且第二液滴可以含有抑制反应的物质时，反应可以开始。

2. 发明申请

WO2004002627A2 METHOD AND APPARATUS FOR FLUID DISPERSION 审中-公开
标题翻译：流体分散方法及装置
公开(公告)号：WO2004002627A2
公开(公告)日：2004-01-08
申请号：PCT/US0320542
申请日：2003-06-30
申请人： HARVARD COLLEGE , STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
发明人： STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
IPC分类号： B01F3/08 , B01F5/06 , B01F13/00 , B01L3/00 , B05B7/04
CPC分类号： B01L3/5027 , B01F3/0807 , B01F5/0682 , B01F5/0688 , B01F13/0062 , B01F2215/0431 , B01F2215/045 , B05B7/0408 , B05B7/0416 , B05B7/0441 , Y10S516/924 , Y10S516/927 , Y10T29/49002 , Y10T137/0324 , Y10T137/0329 , Y10T137/206 , Y10T137/87346 , Y10T436/2575
摘要： A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.
摘要翻译：提供了用于在流体中聚焦和/或形成类似或不同尺寸的不连续部分的微流体方法和装置。该装置可以简单地使用简单的技术从容易获得的廉价材料制造。

3. 发明申请

WO2006096571A3 METHOD AND APPARATUS FOR FORMING MULTIPLE EMULSIONS 审中-公开
标题翻译：形成多种乳剂的方法和设备
公开(公告)号：WO2006096571A3
公开(公告)日：2006-11-23
申请号：PCT/US2006007772
申请日：2006-03-03
申请人： HARVARD COLLEGE , WEITZ DAVID A , LINK DARREN ROY , UTADA ANDREW S
发明人： WEITZ DAVID A , LINK DARREN ROY , UTADA ANDREW S
IPC分类号： B01F13/00 , B01F3/08 , B01F5/04 , B01J13/02
CPC分类号： F15D1/008 , A61K9/113 , A61K9/1273 , A61K9/5089 , B01F3/0807 , B01F5/045 , B01F5/046 , B01F13/0059 , B01F13/0062 , B01F2005/0022 , B82Y10/00 , B82Y30/00 , Y10S516/924 , Y10T137/0391
摘要： The present invention generally relates to multiple emulsions, and to methods and apparatuses for making multiple emulsions. A multiple emulsion generally describes larger droplets that contain one or more smaller droplets therein. The larger droplets may be suspended in a third fluid in some cases. These can be useful for encapsulating species such as pharmaceutical agents, cells, chemicals, or the like. In some cases, one or more of the droplets can change form, for instance, to become solidified to form a microcapsule, a liposome, a polymerosome, or a colloidosome. Multiple emulsions can be formed in one step in certain embodiments, with generally precise repeatability, and can be tailored to include one, two, three, or more inner droplets within a single outer droplet (which droplets may all be nested in some cases).
摘要翻译：本发明一般涉及多重乳液，以及制造多重乳液的方法和设备。多重乳液通常描述其中包含一个或多个较小液滴的较大液滴。在某些情况下，较大的液滴可能会悬浮在第三种液体中。这些可用于封装诸如药剂，细胞，化学品等的物种。在一些情况下，一个或多个液滴可以改变形式，例如变得固化以形成微胶囊，脂质体，聚合物体或胶体体。在某些实施方案中，可以在一个步骤中形成多重乳液，具有通常精确的重复性，并且可以定制为在单个外部液滴内包含一个，两个，三个或更多个内部液滴（该液滴可以全部在一些情况下嵌套）。

4. 发明申请

WO2004002627B1 METHOD AND APPARATUS FOR FLUID DISPERSION 审中-公开
公开(公告)号：WO2004002627B1
公开(公告)日：2004-06-17
申请号：PCT/US0320542
申请日：2003-06-30
申请人： HARVARD COLLEGE , STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
发明人： STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
IPC分类号： B01F3/08 , B01F5/06 , B01F13/00 , B01L3/00 , B05B7/04
CPC分类号： B01L3/5027 , B01F3/0807 , B01F5/0682 , B01F5/0688 , B01F13/0062 , B01F2215/0431 , B01F2215/045 , B05B7/0408 , B05B7/0416 , B05B7/0441 , Y10S516/924 , Y10S516/927 , Y10T29/49002 , Y10T137/0324 , Y10T137/0329 , Y10T137/206 , Y10T137/87346 , Y10T436/2575
摘要： A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

5. 发明申请

WO2007030501A3 MICROFLUIDIC MANIPULATION OF FLUIDS AND REACTIONS 审中-公开
标题翻译：流体的微流体操纵和反应
公开(公告)号：WO2007030501A3
公开(公告)日：2007-10-04
申请号：PCT/US2006034659
申请日：2006-09-07
申请人： UNIV BRANDEIS , HARVARD COLLEGE , FRADEN SETH , LINK DARREN ROY , CRISTOBAL-AZKARATE GALDER , JUNG UK SHIM , WEITZ DAVID A
发明人： FRADEN SETH , LINK DARREN ROY , CRISTOBAL-AZKARATE GALDER , JUNG UK SHIM , WEITZ DAVID A
IPC分类号： C30B13/02 , C30B11/00 , C30B15/26 , C30B15/30
CPC分类号： B01L3/502784 , B01L3/06 , B01L3/502792 , B01L2200/0642 , B01L2200/0673 , B01L2200/10 , B01L2200/14 , B01L2200/141 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2400/0487 , Y10T156/1093 , Y10T436/11 , Y10T436/2575
摘要： The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including microreactors for manipulating fluids and reactions. In some embodiments, structures and methods for manipulating many (e.g., 1000) fluid samples, i.e., in the form of droplets, are described. Processes such as diffusion, evaporation, dilution, and precipitation can be controlled in each fluid sample. These methods also enable conditions within the fluid samples (e.g., concentration) to be controlled. Manipulation of fluid samples can be useful for a variety of applications, including testing for reaction conditions, e.g., in crystallization, chemical, and biological assays.
摘要翻译：本发明一般涉及微流体结构，并且更具体地涉及包括用于操纵流体和反应的微反应器的微流体结构和方法。在一些实施例中，描述了用于操纵许多（例如，1000）流体样本的结构和方法，即以液滴的形式。诸如扩散，蒸发，稀释和沉淀等过程可以在每个流体样品中进行控制。这些方法还能够控制流体样品内的条件（例如浓度）。流体样品的操作可用于多种应用，包括测试反应条件，例如在结晶，化学和生物测定中。

6. 发明申请

WO2004002627A8 METHOD AND APPARATUS FOR FLUID DISPERSION 审中-公开
标题翻译：流体分散方法与装置
公开(公告)号：WO2004002627A8
公开(公告)日：2005-03-17
申请号：PCT/US0320542
申请日：2003-06-30
申请人： HARVARD COLLEGE , STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
发明人： STONE HOWARD A , ANNA SHELLY L , BONTOUX NATHALIE , LINK DARREN ROY , WEITZ DAVID A , GITLIN IRINA , GARSTECKI PIOTR , DILUZIO WILLOW , WHITESIDES GEORGE M , KUMACHEVA EUGENIA
IPC分类号： B01F3/08 , B01F5/06 , B01F13/00 , B01L3/00 , B05B7/04
CPC分类号： B01L3/5027 , B01F3/0807 , B01F5/0682 , B01F5/0688 , B01F13/0062 , B01F2215/0431 , B01F2215/045 , B05B7/0408 , B05B7/0416 , B05B7/0441 , Y10S516/924 , Y10S516/927 , Y10T29/49002 , Y10T137/0324 , Y10T137/0329 , Y10T137/206 , Y10T137/87346 , Y10T436/2575
摘要： A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.
摘要翻译：提供了用于在流体中聚焦和/或形成类似或不同尺寸的不连续部分的微流体方法和装置。该设备可以简单地使用简单的技术从容易获得的廉价材料制造。

7. 发明申请

WO2009134395A3 MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS 审中-公开
标题翻译：用于储存和定义错误布置的微流体装置
公开(公告)号：WO2009134395A3
公开(公告)日：2010-02-18
申请号：PCT/US2009002644
申请日：2009-04-28
申请人： HARVARD COLLEGE , SCHMITZ CHRISTIAN , ROWAT AMY , KOESTER SARAH , AGRESTI JEREMY , WEITZ DAVID A
发明人： SCHMITZ CHRISTIAN , ROWAT AMY , KOESTER SARAH , AGRESTI JEREMY , WEITZ DAVID A
IPC分类号： G01N35/00 , C12Q1/02 , G01N21/64 , G01N33/50
CPC分类号： G01N21/6452 , B01L3/502784 , B01L7/525 , B01L2200/0621 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1827 , B01L2400/0487 , B01L2400/086 , G01N33/5008
摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time- resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
摘要翻译：本发明涉及用于在预定位置中布置液滴的系统和方法。许多应用程序需要收集时间分辨数据。实例包括基于其生长特征或观察酶反应来筛选细胞。本发明提供了一种解决该需求的工具和相关技术，并且可以在许多其它情况下使用。本发明在一个方面提供一种允许单个液滴的稳定储存和分度的工具。本发明不仅可以与微流体/微型设备连接，而且可以与宏观设备接合，以便容易地注入液体和提取样品液滴等。

8. 发明申请

WO2008109176A8 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
标题翻译：测定和涉及滴剂的其他反应
公开(公告)号：WO2008109176A8
公开(公告)日：2008-11-13
申请号：PCT/US2008003185
申请日：2008-03-07
申请人： HARVARD COLLEGE , AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
发明人： AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
IPC分类号： C12Q1/68 , B01J13/00
CPC分类号： C12Q1/6848 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , C12P19/34 , C12Q1/6834 , C12Q1/686
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明一般涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可以用于测定中，并且在某些实施方案中，液滴或乳液可以被硬化以形成凝胶。在一些方面，可以使用凝胶进行非均相测定。例如，可以使小滴硬化以形成凝胶，其中小滴含有细胞，DNA或其他合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以通过凝胶扩散，或者硬化的颗粒可以液化以形成液体状态，从而允许反应物与细胞相互作用。作为具体例子，可以对凝胶颗粒内包含的DNA进行PCR（聚合酶链式反应）扩增，例如通过使用能够在凝胶形成时结合到凝胶上的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

9. 发明申请

WO2008109176A2 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
标题翻译：测量和其他涉及倾销的反应
公开(公告)号：WO2008109176A2
公开(公告)日：2008-09-12
申请号：PCT/US2008003185
申请日：2008-03-07
申请人： HARVARD COLLEGE , AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GUATAM , CHURCH GEORGE
发明人： AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GUATAM , CHURCH GEORGE
CPC分类号： C12Q1/6848 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , C12P19/34 , C12Q1/6834 , C12Q1/686
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明通常涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可用于测定中，并且在某些实施方案中，液滴或乳液可被硬化以形成凝胶。在一些方面，可以使用凝胶进行异质测定。例如，液滴可以被硬化以形成凝胶，其中液滴包含细胞，DNA或其它合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以扩散通过凝胶，或者硬化的颗粒可以液化以形成液体状态，允许反应物与细胞相互作用。作为具体实例，包含在凝胶颗粒内的DNA可以进行PCR（聚合酶链式反应）扩增，例如通过使用能够在形成凝胶时结合凝胶的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

10. 发明申请

WO2012027366A3 ACOUSTIC WAVES IN MICROFLUIDICS 审中-公开
标题翻译： MICROFLUIDICS中的声波
公开(公告)号：WO2012027366A3
公开(公告)日：2012-08-16
申请号：PCT/US2011048804
申请日：2011-08-23
申请人： HARVARD COLLEGE , UNIV AUGSBURG , WEITZ DAVID A , FRANKE THOMAS , WIXFORTH ACHIM , SCHMID LOTHAR , AGRESTI JEREMY , ABATE ADAM R
发明人： WEITZ DAVID A , FRANKE THOMAS , WIXFORTH ACHIM , SCHMID LOTHAR , AGRESTI JEREMY , ABATE ADAM R
IPC分类号： B01J19/10 , C12M1/42
CPC分类号： C12M23/16 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2400/0421 , B01L2400/0436 , B01L2400/0487 , B01L2400/0496 , C12M47/04 , F17D3/01 , G01N2015/1006 , G01N2015/1081 , Y10T137/0391
摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
摘要翻译：本发明的各个方面涉及例如在微流体系统中的流体物质的控制和操纵。在一组实施例中，可以使用表面声波对液滴进行分类。液滴可能含有细胞或其他物种。在一些情况下，表面声波可以使用诸如叉指换能器的表面声波发生器和/或诸如压电基板的材料来产生。除了在液滴分选位置处或附近之外，压电基底可以与微流体基底分离，例如分离成第一或第二微流体通道。在这样的位置处，微流体衬底可以通过一个或多个耦合区域耦合到压电衬底（或其他材料）。在一些情况下，可以实现相对高的分选速率，例如以至少约1,000Hz，至少约10,000Hz或至少约100,000Hz的速率，并且在一些实施方式中，在分选后具有高细胞存活力。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式