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1. 发明申请

WO2005000226A3 MIXED BED MULTI-DIMENSIONAL CHROMATOGRAPHY SYSTEMS AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译：混合床多维色谱系统及其制备和使用方法
公开(公告)号：WO2005000226A3
公开(公告)日：2005-05-06
申请号：PCT/US2004017647
申请日：2004-06-04
申请人： DIVERSA CORP , WEI JING , LATTERICH MARTIN
发明人： WEI JING , LATTERICH MARTIN
IPC分类号： A61K20060101 , G01N30/02 , G01N30/46 , G01N30/60 , G01N30/48
CPC分类号： G01N30/461 , C07K1/13 , C07K1/16 , C07K1/18 , C07K1/36
摘要： The invention provides mixed bed multi-dimensional liquid chromatographs and methods of making and using them. The invention provides systems comprising the mixed bed multi-dimensional liquid chromatographs of the invention operatively linked to mass spectrometry devices. The invention provides novel systems and methods for determining polypeptide profiles and protein expression variations, as with proteome analyses. The present invention provides systems and methods for simultaneously identifying and quantifying individual proteins in complex protein mixtures by selective differential labeling of amino acid residues followed by chromatographic and mass spectrographic analysis. The invention also provides computer program products and computer implemented methods for practicing the systems and methods of the invention.
摘要翻译：本发明提供混合床多维液相色谱仪及其制备和使用方法。本发明提供了包含可操作地连接到质谱分析装置的本发明的混合床多维液相色谱仪的系统。与蛋白质组分析一样，本发明提供了用于确定多肽谱和蛋白质表达变异的新系统和方法。本发明提供了用于通过氨基酸残基的选择性差异标记，然后进行色谱和质谱分析来同时鉴定和定量复杂蛋白质混合物中的单个蛋白质的系统和方法。本发明还提供用于实践本发明的系统和方法的计算机程序产品和计算机实现的方法。

2. 发明申请

WO2003031928A2 CELLULAR ENGINEERING, PROTEIN EXPRESSION PROFILING, DIFFERENTIAL LABELING OF PEPTIDES, AND NOVEL REAGENTS THEREFOR 审中-公开
标题翻译：细胞工程，蛋白质表达谱，肽差异标记及其新型试剂
公开(公告)号：WO2003031928A2
公开(公告)日：2003-04-17
申请号：PCT/US2002/023549
申请日：2002-07-22
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
发明人： SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
IPC分类号： G01N
CPC分类号： G01N33/6848 , G01N33/6818 , G01N2458/15
摘要： The invention provides cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. Furthermore, a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products. Additionally, the invention provides novel methods for determining polypeptide profiles, and protein expression variations, which methods of simultaneously identifying and quantifying individual proteins in complex protein mixtures. Protein expression levels can be globally quantified.
摘要翻译：本发明提供了用于产生具有所需性质的新型转基因生物的细胞转化，定向进化和筛选方法。因此，在一个方面，本发明涉及产生具有差异可激活的多种性状的转基因生物体如微生物或植物的方法。此外，通过引入可在预期宿主中操作的调节序列（例如启动子）来重新组合基因和基因途径的方法，从而在新基因途径被引入预期宿主时赋予其可操作性。例如，基于微生物来源的祖细胞模板产生的新型人造基因途径，其可在植物细胞中操作。此外，产生具有所需性状，重组基因和基因产物的表达增加的新宿主生物的方法。另外，本发明提供了确定多肽概况和蛋白质表达变化的新方法，该方法同时鉴定和定量复合蛋白质混合物中的各种蛋白质。可以全面量化蛋白质表达水平。

3. 发明申请

WO0229032A2 WHOLE CELL ENGINEERING BY MUTAGENIZING A SUBSTANTIAL PORTION OF A STARTING GENOME, COMBINING MUTATIONS, AND OPTIONALLY REPEATING 审中-公开
标题翻译：通过将起始基因组的重要部分，组合突变和可选择重复的整体细胞工程整合
公开(公告)号：WO0229032A2
公开(公告)日：2002-04-11
申请号：PCT/US0131004
申请日：2001-10-01
申请人： DIVERSA CORP , SHORT JAY M , FU PENGCHENG , LATTERICH MARTIN , WEI JING , LEVIN MICHAEL
发明人： SHORT JAY M , FU PENGCHENG , LATTERICH MARTIN , WEI JING , LEVIN MICHAEL
IPC分类号： C12N15/10 , C12N15/82 , G01N33/534 , G01N33/68 , C12N15/00
CPC分类号： C12N15/1089 , C12N15/102 , C12N15/1027 , C12N15/1058 , C12N15/8241 , G01N33/534 , G01N33/6848 , G01N2800/52
摘要： An invention comprising cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. In one embodiment, this invention provides a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. This invention also provides a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, this conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. This invention also provides a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products. This invention provides novel methods for determining polypeptide profiles, and protein expression variations, which methods are applicable to all sample types disclosed herein. The present invention provides methods of simultaneously identifying and quantifying individual proteins in complex protein mixtures. Additionally this invention provides methods for cellular and metabolic engineering of new and modified phenotypes by using "on-line" or "real-time" metabolic flux analysis.
摘要翻译：一种包含细胞转化，定向进化和用于产生具有所需性质的新型转基因生物的筛选方法的发明。在一个实施方案中，本发明提供了产生具有差异可激活的多种性状的转基因生物体如微生物或植物的方法。本发明还提供了通过引入可在预期宿主中操作的调节序列（例如启动子）来重新组合基因和基因途径的方法，当将其导入预期的宿主时，这赋予了新基因途径的可操作性。例如，基于微生物来源的祖细胞模板产生的新型人造基因途径，其可在植物细胞中操作。本发明还提供了产生具有所需性状，重组基因和基因产物表达增加的新型宿主生物的方法。本发明提供了用于确定多肽谱和蛋白质表达变化的新方法，该方法适用于本文公开的所有样品类型。本发明提供同时鉴定和定量复合蛋白质混合物中的各种蛋白质的方法。此外，本发明通过使用“在线”或“实时”代谢通量分析提供新的和修饰的表型的细胞和代谢工程的方法。

4. 发明申请

WO2005000226A2 MIXED BED MULTI-DIMENSIONAL CHROMATOGRAPHY SYSTEMS AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译：混合床多维色谱系统及其制造和使用方法
公开(公告)号：WO2005000226A2
公开(公告)日：2005-01-06
申请号：PCT/US2004/017647
申请日：2004-06-04
申请人： DIVERSA CORPORATION , WEI, Jing , LATTERICH, Martin
发明人： WEI, Jing , LATTERICH, Martin
IPC分类号： A61K
CPC分类号： G01N30/461 , C07K1/13 , C07K1/16 , C07K1/18 , C07K1/36
摘要： The invention provides mixed bed multi-dimensional liquid chromatographs and methods of making and using them. The invention provides systems comprising the mixed bed multi-dimensional liquid chromatographs of the invention operatively linked to mass spectrometry devices. The invention provides novel systems and methods for determining polypeptide profiles and protein expression variations, as with proteome analyses. The present invention provides systems and methods for simultaneously identifying and quantifying individual proteins in complex protein mixtures by selective differential labeling of amino acid residues followed by chromatographic and mass spectrographic analysis. The invention also provides computer program products and computer implemented methods for practicing the systems and methods of the invention.
摘要翻译：本发明提供混合床多维液相色谱仪及其制备和使用方法。本发明提供了包含可操作地连接到质谱装置的本发明的混合床多维液相色谱仪的系统。本发明提供用于确定多肽谱和蛋白质表达变异的新系统和方法，如蛋白质组分析。本发明提供了通过氨基酸残基的选择性差异标记，然后进行色谱和质谱分析来同时鉴定和定量复合蛋白质混合物中的各种蛋白质的系统和方法。本发明还提供了用于实施本发明的系统和方法的计算机程序产品和计算机实现的方法。

5. 发明申请

WO2003031928A3 CELLULAR ENGINEERING, PROTEIN EXPRESSION PROFILING, DIFFERENTIAL LABELING OF PEPTIDES, AND NOVEL REAGENTS THEREFOR 审中-公开
公开(公告)号：WO2003031928A3
公开(公告)日：2003-04-17
申请号：PCT/US2002/023549
申请日：2002-07-22
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
发明人： SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
IPC分类号： G01N33/48
摘要： The invention provides cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. Furthermore, a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products. Additionally, the invention provides novel methods for determining polypeptide profiles, and protein expression variations, which methods of simultaneously identifying and quantifying individual proteins in complex protein mixtures. Protein expression levels can be globally quantified.

6. 发明申请

WO2002029032A3 WHOLE CELL ENGINEERING BY MUTAGENIZING A SUBSTANTIAL PORTION OF A STARTING GENOME, COMBINING MUTATIONS, AND OPTIONALLY REPEATING 审中-公开
公开(公告)号：WO2002029032A3
公开(公告)日：2002-04-11
申请号：PCT/US2001/031004
申请日：2001-10-01
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , FU, Pengcheng , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
发明人： SHORT, Jay, M. , FU, Pengcheng , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
IPC分类号： G01N33/68
摘要： An invention comprising cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. In one embodiment, this invention provides a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. This invention also provides a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, this conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. This invention also provides a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products. This invention provides novel methods for determining polypeptide profiles, and protein expression variations, which methods are applicable to all sample types disclosed herein. The present invention provides methods of simultaneously identifying and quantifying individual proteins in complex protein mixtures. Additionally this invention provides methods for cellular and metabolic engineering of new and modified phenotypes by using "on-line" or "real-time" metabolic flux analysis.

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