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    • 3. 发明申请
    • ISOLATED ALCOHOL DEHYDROGENASE ENZYMES AND USES THEREOF
    • 分离的乙醇脱氢酶的酶及其用途
    • WO2009097346A3
    • 2009-09-24
    • PCT/US2009032258
    • 2009-01-28
    • BIO ARCHITECTURE LAB INCKASHIYAMA YUKI
    • KASHIYAMA YUKI
    • C12N15/53C12N1/15C12N1/19C12N1/21C12N9/04C12P7/58C12P19/02
    • C12N9/0006C12P5/00C12P7/00C12P7/06C12P7/58C12P19/02Y02E50/17Y02T50/678
    • Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.
    • 提供了其中所述多肽具有脱氢酶活性的细菌多核苷酸和多肽,所述脱氢酶活性例如醇脱氢酶(ADH)活性,糖醛酸盐,4-脱氧-L-赤柠-5-己酮糖糖醛酸盐(DEHU)((4S,5S) -4,5-二羟基-2,6-二氧代己酸)氢化酶活性,2-酮-3-脱氧-D-葡糖酸脱氢酶活性,D-甘露糖醛酸氢化酶活性和/或D-甘露糖酸脱氢酶活性。 还提供了用于将多糖例如衍生自生物质的多糖转化为合适的单糖或寡糖以及将合适的单糖或寡糖转化为商品化学品如生物燃料的方法,酶,重组微生物和微生物系统。 还提供了通过本文所述的方法生产的商品化学品。