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1. 发明授权

US09534225B2 Codon optimized nucleic acid encoding a retinitis pigmentosa gtpase regulator (RPGR) 有权
标题翻译：密码子优化的编码视网膜色素变性酶基因调节蛋白（RPGR）的核酸
公开(公告)号：US09534225B2
公开(公告)日：2017-01-03
申请号：US14687227
申请日：2015-04-15
申请人： Guo-Jie Ye , Jilin Liu
发明人： Guo-Jie Ye , Jilin Liu
IPC分类号： C12N9/00 , C12N15/68 , C07K14/47 , C12N15/00 , C12N15/64
CPC分类号： C07K14/4702 , C12N7/00 , C12N9/00 , C12N15/00 , C12N15/64 , C12N15/68 , C12N15/86 , C12N2710/16621 , C12N2750/14143 , C12N2830/008
摘要： This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.
摘要翻译：本发明一般涉及编码色素性视网膜炎GTP酶调节蛋白（RPGR）蛋白质的密码子优化的核酸。相对于编码RPGR蛋白的野生型cDNA，核酸在质粒生产过程中具有增强的稳定性。本发明还涉及包含密码子优化的核酸的表达盒，载体和宿主细胞。还提供了制备包含密码子优化的核酸序列的重组腺相关（rAAV）表达载体的方法。提供的核酸，表达盒，载体和宿主细胞可用于大规模生产用于基因治疗应用的rAAV表达载体。

2. 发明申请

US20170166617A1 CODON OPTIMIZED NUCLEIC ACID ENCODING A RETINITIS PIGMENTOSA GTPASE REGULATOR (RPGR) 审中-公开
公开(公告)号：US20170166617A1
公开(公告)日：2017-06-15
申请号：US15360362
申请日：2016-11-23
申请人： Guo-Jie Ye , Jilin Liu
发明人： Guo-Jie Ye , Jilin Liu
IPC分类号： C07K14/47 , C12N15/86 , C12N7/00
CPC分类号： C07K14/4702 , C12N7/00 , C12N9/00 , C12N15/00 , C12N15/64 , C12N15/68 , C12N15/86 , C12N2710/16621 , C12N2750/14143 , C12N2830/008
摘要： This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.

3. 发明授权

US6120993A 5-oxoprolinase 失效
标题翻译： 5-氧丙泊酚
公开(公告)号：US6120993A
公开(公告)日：2000-09-19
申请号：US762428
申请日：1996-12-09
申请人： Guo-jie Ye , Esther Breslow , Alton Meister, deceased , by Kenneth Meister, executor
发明人： Guo-jie Ye , Esther Breslow , Alton Meister, deceased , by Kenneth Meister, executor
IPC分类号： C12N9/86 , C12Q1/68 , C07H21/04 , C12N15/11 , C12N15/85
CPC分类号： C12N9/86
摘要： The present invention is directed to isolated nucleic acid molecules encoding mammalian 5-oxoprolinase (m 5-OPase). Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for increasing or decreasing the expression of m 5-OPase in host cells. The invention further provides a method of screening a substance for the ability of the substance to modify m 5-OPase function, and a method for isolating other m 5-OPase molecules. DNA oligomers and antibodies specific for m 5-OPase are provided, each of which can be used to detect m 5-OPase in a sample.
摘要翻译：本发明涉及编码哺乳动物5-氧代罗维拉酸酶（m 5-OPase）的分离的核酸分子。还提供了包含核酸分子的表达载体和宿主细胞，以及用于增加或降低宿主细胞中m 5 -OPase表达的方法。本发明还提供了筛选物质以修饰m 5-OPase功能的能力的方法，以及分离其它m 5-OPase分子的方法。提供DNA寡聚体和针对m 5-OPase特异性的抗体，其中每一种可用于检测样品中的5-OPase。

4. 发明申请

US20140364486A1 PROMOTERS, EXPRESSION CASSETTES, VECTORS, KITS, AND METHODS FOR THE TREATMENT OF ACHROMATOPSIA AND OTHER DISEASES 有权
标题翻译：促进剂，表达CASSETTES，载体，作用和治疗丙型肝炎及其他疾病的方法
公开(公告)号：US20140364486A1
公开(公告)日：2014-12-11
申请号：US14269723
申请日：2014-05-05
申请人： Guo-Jie Ye
发明人： Guo-Jie Ye
IPC分类号： C12N15/85
CPC分类号： A61K48/00 , A61K48/0058 , C12N15/86 , C12N2750/14141 , C12N2830/008
摘要： The present invention provides isolated promoters, transgene expression cassettes, vectors, kits, and methods for treatment of genetic diseases that affect the cone cells of the retina.
摘要翻译：本发明提供分离的启动子，转基因表达盒，载体，试剂盒和用于治疗影响视网膜的锥细胞的遗传疾病的方法。

5. 发明授权

US06451704B1 Method for forming PLDD structure with minimized lateral dopant diffusion 有权
标题翻译：用最小化横向掺杂剂扩散形成PLDD结构的方法
公开(公告)号：US06451704B1
公开(公告)日：2002-09-17
申请号：US09849672
申请日：2001-05-07
申请人： Yelehanka Ramachandramurthy Pradeep , Subrahmanyam Chivukula , Jie Ye , Madhudsudan Mukhopdhyay
发明人： Yelehanka Ramachandramurthy Pradeep , Subrahmanyam Chivukula , Jie Ye , Madhudsudan Mukhopdhyay
IPC分类号： H01L2100
CPC分类号： H01L29/6659 , H01L21/823814 , H01L29/6656
摘要： A new method is provided for the creation of PLDD regions that is aimed at reducing lateral p-type impurity diffusion. The process starts with a silicon substrate on the surface of which gate electrodes have been created. An NLDD implantation is performed self-aligned with the NMOS gate electrode, a layer of oxide (oxide liner) is deposited over the structure over which a layer of nitride is deposited over which a first layer of top oxide is deposited. First gate spacers are formed by etching the first layer of top oxide, stopping on the nitride layer. NS/D and PS/D implantations are performed self-aligned with respectively the NMOS and the PMOS devices, the S/D implantations are annealed. The first gate oxide spacers are removed, a PLDD implantation is performed self-aligned with the PMOS gate electrode. A second layer of top oxide is deposited over the structure and etched to form the second gate spacers on the sidewalls of the NMOS and PMOS gate electrodes. After this sequence of processing steps has been completed, the gate electrodes can be completed following conventional methods of gate electrode processing.
摘要翻译：提供了一种用于创建旨在减少横向p型杂质扩散的PLDD区域的新方法。该工艺从在其上形成栅电极的表面上的硅衬底开始。执行NIGD注入与NMOS栅电极自对准，一层氧化物（氧化物衬垫）沉积在其上沉积有一层氮化物的结构上，第一层顶层氧化物沉积在该层上。通过蚀刻顶部氧化物的第一层，停止在氮化物层上形成第一栅极间隔物。 NS / D和PS / D注入分别与NMOS和PMOS器件自对准，S / D注入退火。去除第一栅极氧化物间隔物，执行PLDD注入与PMOS栅电极自对准。在结构上沉积第二层顶部氧化物，并被蚀刻以在NMOS和PMOS栅电极的侧壁上形成第二栅极间隔物。在该处理步骤顺序完成之后，可以按照常规的栅电极处理方法来完成栅电极。

6. 发明授权

US09724429B2 Promoters, expression cassettes, vectors, kits, and methods for the treatment of achromatopsia and other diseases 有权
公开(公告)号：US09724429B2
公开(公告)日：2017-08-08
申请号：US14269723
申请日：2014-05-05
申请人： Guo-Jie Ye
发明人： Guo-Jie Ye
IPC分类号： C12N15/85 , A61K48/00 , C12N15/86
CPC分类号： A61K48/00 , A61K48/0058 , C12N15/86 , C12N2750/14141 , C12N2830/008
摘要： The present invention provides isolated promoters, transgene expression cassettes, vectors, kits, and methods for treatment of genetic diseases that affect the cone cells of the retina.

7. 发明申请

US20150353938A1 CODON OPTIMIZED NUCLEIC ACID ENCODING A RETINITIS PIGMENTOSA GTPASE REGULATOR (RPGR) 有权
标题翻译： CODON优化核酸编码重组腺嘌呤激酶调节因子（RPGR）
公开(公告)号：US20150353938A1
公开(公告)日：2015-12-10
申请号：US14687227
申请日：2015-04-15
申请人： Guo-Jie Ye , Jilin Liu
发明人： Guo-Jie Ye , Jilin Liu
IPC分类号： C12N15/68 , C07K14/47
CPC分类号： C07K14/4702 , C12N7/00 , C12N9/00 , C12N15/00 , C12N15/64 , C12N15/68 , C12N15/86 , C12N2710/16621 , C12N2750/14143 , C12N2830/008
摘要： This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.
摘要翻译：本发明一般涉及编码色素性视网膜炎GTP酶调节蛋白（RPGR）蛋白质的密码子优化的核酸。相对于编码RPGR蛋白的野生型cDNA，核酸在质粒生产过程中具有增强的稳定性。本发明还涉及包含密码子优化的核酸的表达盒，载体和宿主细胞。还提供了制备包含密码子优化的核酸序列的重组腺相关（rAAV）表达载体的方法。所提供的核酸，表达盒，载体和宿主细胞可用于大规模生产用于基因治疗应用的rAAV表达载体。

8. 发明申请

US20130317091A1 PROMOTERS, EXPRESSION CASSETTES, VECTORS, KITS, AND METHODS FOR THE TREATMENT OF ACHROMATOPSIA AND OTHER DISEASES 有权
标题翻译：促进剂，表达CASSETTES，载体，作用和治疗丙型肝炎及其他疾病的方法
公开(公告)号：US20130317091A1
公开(公告)日：2013-11-28
申请号：US13936728
申请日：2013-07-08
申请人： Guo-Jie Ye , Jeffrey D. Chulay
发明人： Guo-Jie Ye , Jeffrey D. Chulay
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , A61K48/0058 , C07K14/705 , C12N2799/025 , C12N2830/008
摘要： The present invention provides isolated promoters, transgene expression cassettes, vectors, kits, and methods for treatment of genetic diseases that affect the cone cells of the retina.
摘要翻译：本发明提供分离的启动子，转基因表达盒，载体，试剂盒和用于治疗影响视网膜的锥细胞的遗传疾病的方法。

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