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1. 发明申请

WO2009136676A1 METHOD FOR SPECIFIC COVALENT COUPLING OF ANTIBODY USING A PHOTOACTIVABLE PROTEIN G VARIANT 审中-公开
标题翻译：使用光化学蛋白G变体进行抗体的特异性共价连接的方法
公开(公告)号：WO2009136676A1
公开(公告)日：2009-11-12
申请号：PCT/KR2008/002956
申请日：2008-05-27
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
发明人： CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
IPC分类号： C07K14/315
CPC分类号： C07K14/315 , G01N33/531 , G01N33/533
摘要： The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor.
摘要翻译：本发明涉及包含通过用半胱氨酸代替蛋白G的Fc结合结构域的特异性残基制备的突变Fc结合结构域的蛋白G变体及其制备方法。此外，本发明涉及包含用UV交联剂位点选择性修饰的半胱氨酸突变的Fc结合结构域的蛋白G变体。此外，本发明涉及用抗体将蛋白G变体UV交联的方法。本发明涉及通过上述方法制备的蛋白G变体 - 抗体缀合物。此外，本发明提供了使用该缀合物筛选或分析抗原的方法。此外，本发明提供通过将蛋白G变体与固体支持物的表面连接而制造的生物芯片或生物传感器及其制造方法。此外，本发明提供了使用生物芯片或生物传感器固定抗体和分析抗原的方法。

2. 发明申请

WO2012033382A3 NOVEL RNA-BINDING PROTEIN AND LABEL-FREE DETECTION METHOD FOR DETECTING MICRORNA USING SAME 审中-公开
标题翻译：新型RNA结合蛋白和用于检测MICRORNA的无标记检测方法
公开(公告)号：WO2012033382A3
公开(公告)日：2012-06-28
申请号：PCT/KR2011006710
申请日：2011-09-09
申请人： KOREA RES INST OF BIOSCIENCE , JUNG YONG WON , CHUNG BONG HYUN , LEE JEONG MIN
发明人： JUNG YONG WON , CHUNG BONG HYUN , LEE JEONG MIN
IPC分类号： C07K19/00 , C12N15/62 , C12Q1/68 , G01N33/68
CPC分类号： C12Q1/6809 , C07K14/47 , C07K14/52 , C07K2319/00 , C12N9/22 , C12Q2522/101 , C12Q2525/207
摘要： The present invention relates to a novel RNA-binding protein, to a composition comprising same for detecting microRNA, and to a label-free detection method for detecting microRNA using same. According to the present invention, the method for detecting microRNA using the RNA-binding protein, which is a fusion protein comprising a PAZ domain and a double stranded RNA-binding domain and which binds to double stranded RNA having an overhanging 3' terminal, is a label-free detection method for detecting microRNA without an enzymatic labeling process or enzymatic amplifying reaction, which may overcome the drawbacks of conventional microRNA analysis methods, that is, methods for analyzing microRNA after enzymatic labeling or methods for coupling an antibody to microRNA, wherein the drawbacks may include complicated processes, or difficulties developing an RNA-structure-specific antibody resulting from an instable RNA structure. Thus, the method of the present invention may be utilized in the study of gene expression in a variety of diseases, in the detection of diagnostic markers of diseases, and in the evaluation of drug efficacy.
摘要翻译：本发明涉及一种新型RNA结合蛋白，涉及包含其的用于检测微小RNA的组合物以及使用其检测微小RNA的无标记检测方法。根据本发明，使用作为包含PAZ结构域和双链RNA结合结构域并与具有突出端3'末端的双链RNA结合的融合蛋白的RNA结合蛋白检测微小RNA的方法是用于检测没有酶标记过程或酶促扩增反应的微RNA的无标记检测方法，其可以克服常规微RNA分析方法的缺点，即在酶标记之后分析微小RNA的方法或将抗体偶联到微小RNA上的方法，其中缺点可能包括复杂的过程，或由不稳定的RNA结构产生的RNA结构特异性抗体的困难。因此，本发明的方法可用于研究各种疾病中的基因表达，检测疾病的诊断标志物和药物功效的评价。

3. 发明申请

WO2008156249A1 PROTEIN G-OLIGONUCLEOTIDE CONJUGATE 审中-公开
标题翻译：蛋白G-寡核苷酸结合蛋白
公开(公告)号：WO2008156249A1
公开(公告)日：2008-12-24
申请号：PCT/KR2008/002739
申请日：2008-05-16
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
发明人： CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
IPC分类号： C07K19/00
CPC分类号： C07K19/00 , C07K14/315 , G01N33/54393
摘要： The present invention relates to a protein G conjugate, which is prepared by linking an N-terminal cysteine-tagged protein G variant with an oligonucleotide via a linker. The conjugate binds in a directional manner on the surface of a biochip and biosensor, thereby providing a biochip and biosensor having improved antibody immobilization ability.
摘要翻译：本发明涉及蛋白G缀合物，其通过将N-末端半胱氨酸标记的蛋白G变体与寡核苷酸通过接头连接来制备。缀合物以有向的方式结合在生物芯片和生物传感器的表面上，从而提供具有改进的抗体固定能力的生物芯片和生物传感器。

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