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    • 5. 发明授权
    • Method of containing core material in microcapsules
    • 在微胶囊中含有核心材料的方法
    • US5795570A
    • 1998-08-18
    • US418953
    • 1995-04-07
    • Collin J. WeberJennifer Ayres-Price
    • Collin J. WeberJennifer Ayres-Price
    • A01N1/02A61K9/10A61K9/50A61K35/12A61K35/14A61K35/30A61K35/39A61K35/407A61P3/08A61P3/10A61P3/14C12N5/00C12N5/071C12N11/04C12N11/10B01J13/20
    • A61K9/5073C12N11/04C12N5/0012C12N5/0677A61K2035/126A61K2035/128C12N2533/74Y10T428/2984Y10T428/2989
    • A core material such as animal tissue or cells is contained within a semipermeable vessel which may be a microcapsule, hollow fiber or plastic membrane having a semipermeable wall by a method that prevents the core material from incorporation into the wall of the vessel. This is accomplished by suspending the core material in a solution of polysaccharide gum such as an alkali metal alginate in an amount between about 0.2% and about 0.5%, removing and washing the core material to remove all but a thin layer of polysaccharide gum, and gelling the polysaccharide gum with multivalent cations or other means to form a pretreated core material. The pretreated core material is contained within a semipermeable vessel such as by suspending the pretreated core material in a solution of alkali metal alginate, forming the suspension into droplets, gelling the droplets to form temporary shape-retaining capsules and treating the capsules with a polymeric material having groups that react with and crosslink acid groups of the capsules to form a permanent semipermeable membrane around the capsules. A second permanent semipermeable membrane may be formed around the capsules to form double-walled microcapsules by further treating the capsules with the polymeric material. The semipermeable vessel may be impermeable to immune factors. Cells or tissue can be transplanted from a donor to a subject such as by using pancreatic islet tissue or cells as the core material of the double-walled microcapsules and transplanting the microcapsules by intraperitoneal injection into a diabetic subject.
    • 诸如动​​物组织或细胞的核心材料包含在半透膜容器内,该半透膜容器可以是具有半透壁的微胶囊,中空纤维或塑料膜,其方法是防止芯材掺入容器的壁中。 这是通过将核心材料悬浮在多糖胶(例如碱金属藻酸盐)的溶液中,其量为约0.2%至约0.5%,除去和洗涤核心材料以除去除了多糖胶质的薄层以外的所有物质,以及 用多价阳离子或其他方式胶凝多糖胶,以形成预处理的芯材料。 将预处理的芯材料包含在半透性容器内,例如通过将预处理的芯材料悬浮在碱金属藻酸盐的溶液中,将悬浮液形成液滴,使液滴胶凝以形成暂时的形状保持胶囊,并用聚合物材料 具有与胶囊的酸基团反应并交联的基团,以在胶囊周围形成永久的半透膜。 通过用聚合物材料进一步处理胶囊,可以在胶囊周围形成第二永久半透膜以形成双壁微胶囊。 半透明容器可能是不可渗透的免疫因素。 可以将细胞或组织从供体移植到受试者,例如通过使用胰岛组织或细胞作为双壁微胶囊的核心材料,并将微胶囊通过腹膜内注射移植到糖尿病受试者中。