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1. 发明申请

US20130210074A1 EPIGENETIC ENGINEERING 审中-公开
标题翻译：仿生工程
公开(公告)号：US20130210074A1
公开(公告)日：2013-08-15
申请号：US13859097
申请日：2013-04-09
申请人： Lore FLORIN , Barbara ENENKEL , Martin FUSSENEGGER , Hitto KAUFMANN , Raffaella SANTORO
发明人： Lore FLORIN , Barbara ENENKEL , Martin FUSSENEGGER , Hitto KAUFMANN , Raffaella SANTORO
IPC分类号： C12N15/113 , C12N15/85 , C12P21/00
CPC分类号： C12N15/1136 , C12N15/111 , C12N15/113 , C12N15/85 , C12N2310/14 , C12P21/00
摘要： The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines.The invention further concerns a method of producing proteins using the cells generated by the described method.
摘要翻译：本发明涉及细胞培养技术领域。它涉及通过减少NoCR蛋白表达，特别是TIP-5而实现的核糖体RNA（rRNA）表达增加的生产宿主细胞系。与对照细胞系相比，这些细胞系具有改善的分泌和生长特性。本发明还涉及使用由所述方法产生的细胞产生蛋白质的方法。

2. 发明申请

US20080300207A1 PROTEIN PRODUCTION 有权
标题翻译：蛋白质生产
公开(公告)号：US20080300207A1
公开(公告)日：2008-12-04
申请号：US12040198
申请日：2008-02-29
申请人： Hitto Kaufmann , Lore Florin , Eric Becker , Monilola Olayioye , Angelika Hausser , Tim Fugmann
发明人： Hitto Kaufmann , Lore Florin , Eric Becker , Monilola Olayioye , Angelika Hausser , Tim Fugmann
IPC分类号： A61K31/70 , C12P21/04 , C12N5/06 , C12N15/00 , C12N1/19 , A61P31/00 , C12Q1/02 , C12N5/04 , C07K7/00 , C07K16/18
CPC分类号： C12N15/63 , C07K14/435 , C07K14/47 , C07K14/4702 , C07K16/00 , C07K2319/036 , C12N15/67 , C12N15/85 , C12P21/00 , C12Q1/68 , G01N33/68
摘要： The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.
摘要翻译：本发明涉及蛋白质生产和细胞培养技术领域。 CERT被确定为体内PKD底物的新颖。通过PKD在丝氨酸132上的磷酸化降低了CERT对高尔基体膜上的脂质靶磷脂酰肌醇4-磷酸的亲和力，降低了神经酰胺转移活性，鉴定了PKD作为脂质体内平衡的调节剂。本发明显示CERT又对于PKD激活和PKD依赖蛋白质运送到质膜的关键。因此，PKD和CERT的相互依存关系是维持高尔基体膜完整性和分泌转运的关键。

3. 发明授权

US08535940B2 Cell growth 有权
标题翻译：细胞生长
公开(公告)号：US08535940B2
公开(公告)日：2013-09-17
申请号：US12524219
申请日：2008-01-22
申请人： Hitto Kaufmann , Lore Florin , Eric Becker
发明人： Hitto Kaufmann , Lore Florin , Eric Becker
IPC分类号： A61K39/395 , C12N5/10 , A61P35/00 , C12P21/00
CPC分类号： C12N5/0602 , C12N9/003 , C12N2501/70 , C12N2501/998 , C12N2510/02
摘要： The invention concerns the field of cell culture technology. It concerns a method of improving cell growth, especially the growth of biopharmaceutical producer host cells. The invention further concerns a method of producing proteins using the cells generated by the described method.
摘要翻译：本发明涉及细胞培养技术领域。它涉及改善细胞生长的方法，特别是生物制药生产者宿主细胞的生长。本发明还涉及使用由所述方法产生的细胞产生蛋白质的方法。

4. 发明申请

US20130197196A1 PROTEIN PRODUCTION 审中-公开
公开(公告)号：US20130197196A1
公开(公告)日：2013-08-01
申请号：US13494402
申请日：2012-06-12
申请人： Hitto KAUFMANN , Lore FLORIN , Eric BECKER , Monilola OLAYIOYE , Angelika HAUSSER , Tim FUGMANN
发明人： Hitto KAUFMANN , Lore FLORIN , Eric BECKER , Monilola OLAYIOYE , Angelika HAUSSER , Tim FUGMANN
IPC分类号： C07K16/00 , C07K14/435
CPC分类号： C12N15/63 , C07K14/435 , C07K14/47 , C07K14/4702 , C07K16/00 , C07K2319/036 , C12N15/67 , C12N15/85 , C12P21/00 , C12Q1/68 , G01N33/68
摘要： The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.

5. 发明申请

US20130177919A1 PROTEIN PRODUCTION 审中-公开
公开(公告)号：US20130177919A1
公开(公告)日：2013-07-11
申请号：US12528828
申请日：2008-02-29
申请人： Hitto Kaufmann , Lore Florin , Eric Becker , Monilola Olayioye , Angelika Hausser , Tim Fugmann
发明人： Hitto Kaufmann , Lore Florin , Eric Becker , Monilola Olayioye , Angelika Hausser , Tim Fugmann
IPC分类号： C12N15/85 , G01N33/68 , C12Q1/68 , C07K14/47 , C12P21/00
CPC分类号： C12N15/63 , C07K14/435 , C07K14/47 , C07K14/4702 , C07K16/00 , C07K2319/036 , C12N15/67 , C12N15/85 , C12P21/00 , C12Q1/68 , G01N33/68
摘要： The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.

6. 发明申请

US20120100553A1 CHO/CERT CELL LINES 有权
标题翻译： CHO / CERT细胞系
公开(公告)号：US20120100553A1
公开(公告)日：2012-04-26
申请号：US13318509
申请日：2010-05-04
申请人： Lore Florin , Eric Becker , Hitto Kaufmann
发明人： Lore Florin , Eric Becker , Hitto Kaufmann
IPC分类号： C12N5/10 , C12P21/00 , C12Q1/68
CPC分类号： C07K14/47
摘要： The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).
摘要翻译：本发明涉及细胞培养技术领域。本发明描述了包含载体构建体的生产宿主细胞系，其包含CERT S132A表达盒。这些细胞系具有改善的生长特性和较高的CERT S132A表达水平。本发明特别涉及以DSM ACC2989（CHO / CERT 2.20）和DSM AC-C2990（CHO / CERT 2.41）存放于DSMZ的两种细胞系。本发明还涉及产生这种优选的生产宿主细胞的方法，以及使用保藏在帝斯曼数据DSM ACC2989（CHO / CERT 2.20）和DSM ACC2990（CHO / CERT 2.41）上的DSMZ的两种细胞系产生蛋白质的方法。

7. 发明授权

US09340592B2 CHO/CERT cell lines 有权
标题翻译： CHO / CERT细胞系
公开(公告)号：US09340592B2
公开(公告)日：2016-05-17
申请号：US13318509
申请日：2010-05-04
申请人： Lore Florin , Eric Becker , Hitto Kaufmann
发明人： Lore Florin , Eric Becker , Hitto Kaufmann
IPC分类号： C12N15/85 , C12N15/63 , A61K31/711 , C12P21/08 , C07K14/47
CPC分类号： C07K14/47
摘要： The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).
摘要翻译：本发明涉及细胞培养技术领域。本发明描述了包含载体构建体的生产宿主细胞系，其包含CERT S132A表达盒。这些细胞系具有改善的生长特性和较高的CERT S132A表达水平。本发明特别涉及以DSM ACC2989（CHO / CERT 2.20）和DSM AC-C2990（CHO / CERT 2.41）存放于DSMZ的两种细胞系。本发明还涉及产生这种优选的生产宿主细胞的方法，以及使用保藏在帝斯曼数据DSM ACC2989（CHO / CERT 2.20）和DSM ACC2990（CHO / CERT 2.41）上的DSMZ的两种细胞系产生蛋白质的方法。

8. 发明授权

US08563303B2 Method for increasing recloning efficiency 有权
标题翻译：提高再生能力的方法
公开(公告)号：US08563303B2
公开(公告)日：2013-10-22
申请号：US12922945
申请日：2009-03-16
申请人： Jüergen Fieder , Lore Florin , Hitto Kaufmann , Thomas Krieg , Melanie Briegel
发明人： Jüergen Fieder , Lore Florin , Hitto Kaufmann , Thomas Krieg , Melanie Briegel
IPC分类号： C12N5/02 , C12N5/07 , C12N5/18
CPC分类号： C12N5/0037 , C12N2500/84 , C12N2501/105 , C12N2502/243 , C12N2510/02
摘要： The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells.By using IGF particularly in conjunction with HSA in the culture medium after recloning, the recloning efficiency and hence the quantity of clones obtained can be increased significantly.
摘要翻译：本发明涉及细胞培养技术领域，涉及复制/克隆细胞的方法，优选细胞系对于生产生物药物的生产是重要的。本发明还涉及使用已经通过单细胞沉积获得和复制的细胞制备蛋白质的方法，以及使得能够复制个体细胞的组合物。通过使用IGF，特别是与再生后的培养基中的HSA结合，可以显着提高所获得的克隆的再生能力和因此的量。

9. 发明申请

US20120258496A1 PRODUCTION OF LOW FUCOSE ANTIBODIES IN H4-II-E RAT CELLS 审中-公开
标题翻译：在H4-II-E细胞中生产低FUCIOS抗体
公开(公告)号：US20120258496A1
公开(公告)日：2012-10-11
申请号：US13239654
申请日：2011-09-22
申请人： Kristina Ellwanger , Lore Florin , Hitto Kaufmann
发明人： Kristina Ellwanger , Lore Florin , Hitto Kaufmann
IPC分类号： C12P21/00 , C12N5/10 , C12N15/85
CPC分类号： C12P21/005 , C07K16/2887 , C07K16/32 , C07K2317/14 , C07K2317/41 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C07K2319/30 , C12N5/067 , C12N2500/90 , C12N2510/02
摘要： The invention concerns the field of cell culture technology. It specifically concerns a rat hepatoma cell, preferably a H4-II-E rat hepatoma cell, carrying a DNA encoding an antibody or Fc-fusion protein and having low fucosylation activity for adding fucose to glycosidic structures such as biantennary glycans, e.g. N-acetylglucosamine. The invention furthermore concerns a method for producing low fucose glycoproteins especially antibodies or Fc-fusion proteins in rat hepatoma cells, preferably in H4-II-E rat hepatoma cells. It further concerns the identification and generation of new host cell lines which are capable of synthetizing glycoproteins with beneficial properties, improving the therapeutic efficacy and/or serum half-life of the product compared to products from commonly used host cell lines.
摘要翻译：本发明涉及细胞培养技术领域。它具体涉及一种大鼠肝癌细胞，优选H4-II-E大鼠肝癌细胞，其携带编码抗体或Fc融合蛋白的DNA，并且具有低岩藻糖基化活性，以将岩藻糖加入到糖苷结构中，例如双天线聚糖，例如双链聚糖。 N-乙酰氨基葡萄糖。本发明还涉及在大鼠肝癌细胞，优选在H4-II-E大鼠肝癌细胞中生产低岩藻糖糖蛋白，特别是抗体或Fc-融合蛋白的方法。它还涉及与来自常用宿主细胞系的产物相比，鉴定和产生能够合成具有有益性质的糖蛋白的新宿主细胞系，提高产品的治疗功效和/或血清半衰期。

10. 发明申请

US20100086967A1 CELL GROWTH 有权
标题翻译：细胞生长
公开(公告)号：US20100086967A1
公开(公告)日：2010-04-08
申请号：US12524219
申请日：2008-01-22
申请人： Hitto Kaufmann , Lore Florin , Eric Becker
发明人： Hitto Kaufmann , Lore Florin , Eric Becker
IPC分类号： C12P21/00 , C12N5/00 , C12N15/00 , C12N5/07
CPC分类号： C12N5/0602 , C12N9/003 , C12N2501/70 , C12N2501/998 , C12N2510/02
摘要： The invention concerns the field of cell culture technology. It concerns a method of improving cell growth, especially the growth of biopharmaceutical producer host cells. The invention further concerns a method of producing proteins using the cells generated by the described method.
摘要翻译：本发明涉及细胞培养技术领域。它涉及改善细胞生长的方法，特别是生物制药生产者宿主细胞的生长。本发明还涉及使用由所述方法产生的细胞产生蛋白质的方法。

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