基本信息:
- 专利标题： PROTEIN PRODUCTION
- 申请号：US13494402 申请日：2012-06-12
- 公开(公告)号：US20130197196A1 公开(公告)日：2013-08-01
- 发明人： Hitto KAUFMANN , Lore FLORIN , Eric BECKER , Monilola OLAYIOYE , Angelika HAUSSER , Tim FUGMANN
- 申请人： Hitto KAUFMANN , Lore FLORIN , Eric BECKER , Monilola OLAYIOYE , Angelika HAUSSER , Tim FUGMANN
- 申请人地址： DE Ingelheim am Rhein
- 专利权人： BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG
- 当前专利权人： BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG
- 当前专利权人地址： DE Ingelheim am Rhein
- 优先权： EP07103406.0 20070302; EP07104226.1 20070315; EP07116358.8 20070913
- 主分类号： C07K16/00
- IPC分类号： C07K16/00 ; C07K14/435
摘要：
The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.