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    • 5. 发明授权
    • Replication of hepatitis C virus genome and identification of virus
having high infectivity
    • 复制丙型肝炎病毒基因组和识别感染性高的病毒
    • US5766919A
    • 1998-06-16
    • US654128
    • 1996-05-28
    • Hiroshi YoshikuraYohko ShimizuAikichi IwamotoMinako Hijikata
    • Hiroshi YoshikuraYohko ShimizuAikichi IwamotoMinako Hijikata
    • C12N7/00C12Q1/04G01N33/50G01N33/576C12N7/01
    • G01N33/5767C12N7/00C12Q1/04G01N33/5091C12N2770/24221C12N2770/24251G01N2333/18
    • A method for replication of hepatitis C virus genome comprising infecting animal cells that have been infected with mouse retrovirus, with hepatitis C virus, and then culturing those cells. The present invention further provides a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising centrifugating a sample comprising a virus to be tested in a liquid medium to determine the density of the virus, and assigning the virus to a hepatitis C virus with high infectivity it the density thereof is up to about 1.06 g/ml, and assigning the virus to a hepatitis C virus with less infectivity if the density thereof is at least 1.13 g/ml; a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising bringing a virus to be tested into contact with an anti-human immunoglobulin, and assigning the virus to hepatitis C virus with high infectivity if the virus does not form an immunocomplex and assigning the virus to a hepatitis C virus with less infectivity if it does form an immunocomplex; and a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising inoculating a virus to be tested to cells infected with murine retrovirus, culturing the cells, and assigning the virus to a hepatitis C virus with high infectivity if the virus replicates in the cells, or assigning the virus to a hepatitis C virus with less infectivity if the virus does not replicate in the cells.
    • 一种用于复制丙型肝炎病毒基因组的方法,包括用丙型肝炎病毒感染已被小鼠逆转录病毒感染的动物细胞,然后培养这些细胞。 本发明还提供了一种鉴别具有较高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法,包括在液体培养基中离心包含待测试病毒的样品以确定病毒的密度, 病毒感染性高的丙型肝炎病毒,其密度高达约1.06μg/ ml,并且如果其密度至少为1.13μg/ ml,则将该病毒分配给感染性较差的丙型肝炎病毒; 一种区分具有高感染性的丙型肝炎病毒和较少感染性的丙型肝炎病毒的方法,包括使待测试的病毒与抗人免疫球蛋白接触,并将病毒分配给感染性高的丙型肝炎病毒,如果病毒 如果它形成免疫复合物,则不形成免疫复合物并将病毒分配给具有较少感染性的丙型肝炎病毒; 以及用于区分具有高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法,包括将待测试的病毒接种到用鼠逆转录病毒感染的细胞,培养细胞,并将病毒分配给具有高度感染性的丙型肝炎病毒 如果病毒在细胞中复制,或者如果病毒不在细胞中复制,则将病毒分配给具有较少感染性的丙型肝炎病毒的感染性。
    • 9. 发明授权
    • Replication of hepatitis C virus genome and identification of virus
having high infectivity
    • 复制丙型肝炎病毒基因组和识别感染性高的病毒
    • US5552310A
    • 1996-09-03
    • US963490
    • 1992-10-20
    • Hiroshi YoshikuraYohko ShimizuAikichi IwamotoMinako Hijikata
    • Hiroshi YoshikuraYohko ShimizuAikichi IwamotoMinako Hijikata
    • C12N7/00C12Q1/04G01N33/50G01N33/576C12Q1/70C12N7/01C12N7/02
    • G01N33/5767C12N7/00C12Q1/04G01N33/5091C12N2770/24221C12N2770/24251G01N2333/18
    • A method for replication of hepatitis C virus genome comprising infecting animal cells that have been infected with mouse retrovirus, with hepatitis C virus, and then culturing those cells. The present invention further provides a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising centrifugating a sample comprising a virus to be tested in a liquid medium to determine the density of the virus, and assigning the virus to a hepatitis C virus with high infectivity if the density thereof is up to about 1.06 g/ml, and assigning the virus to a hepatitis C virus with less infectivity if the density thereof is at least 1.13 g/ml; a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising brining a virus to be tested into contact with an anti-human immunoglobulin, and assigning the virus to hepatitis C virus with high infectivity if it does not form an immunocomplex and assigning the virus to a hepatitis C virus with less infectivity if it does form an immunocomplex; and a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising inoculating a virus to be tested to cells infected with murine retrovirus, culturing the cells, and assigning the virus to a hepatitis C virus with high infectivity if the virus replicates in the cells, or assigning the virus to a hepatitis C virus with less infectivity if the virus does not replicate in the cells.
    • 一种用于复制丙型肝炎病毒基因组的方法,包括用丙型肝炎病毒感染已被小鼠逆转录病毒感染的动物细胞,然后培养这些细胞。 本发明还提供了一种鉴别具有较高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法,包括在液体培养基中离心包含待测试病毒的样品以确定病毒的密度, 如果其密度高达约1.06μg/ ml,则具有高感染性的丙型肝炎病毒,如果其密度至少为1.13μg/ ml,则将病毒分配给具有较低感染性的丙型肝炎病毒; 一种区分具有高感染性的丙型肝炎病毒和较少感染性的丙型肝炎病毒的方法,包括将待测试的病毒与抗人免疫球蛋白接触,并将该病毒分配给感染性高的丙型肝炎病毒 不形成免疫复合物,如果确实形成免疫复合物,则将病毒分配给感染性较差的丙型肝炎病毒; 以及用于区分具有高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法,包括将待测试的病毒接种到用鼠逆转录病毒感染的细胞,培养细胞,并将病毒分配给具有高度感染性的丙型肝炎病毒 如果病毒在细胞中复制,或者如果病毒不在细胞中复制,则将病毒分配给具有较少感染性的丙型肝炎病毒的感染性。
    • 10. 发明授权
    • Vortex flowmeter
    • 涡街流量计
    • US4924710A
    • 1990-05-15
    • US354222
    • 1989-05-19
    • Yutaka InadaKazumasa KawasakiShigeru NishiyamaHiroshi Yoshikura
    • Yutaka InadaKazumasa KawasakiShigeru NishiyamaHiroshi Yoshikura
    • G01F1/32
    • G01F1/3281
    • A vortex flowmeter comprising ultrasonic wave transmitters and ultrasonic wave receivers adapted to receive ultrasonic waves transmitted by the transmitters through a flow of fluid in which vortices are generated by a vortex generator, the ultrasonic wave signals output by the ultrasonic wave receivers being compared by a phase comparator to measure the flow rate of the fluid by detecting the difference in phase of the ultrasonic waves due to generation of the vortices. First and second ultrasonic wave transmitters are disposed on the wall of a pipe line defining the flow path for the fluid downstream of the vortex generator and first and second ultrasonic wave receivers are respectively disposed at positions on the wall of the pipe line opposite to the first and second transmitters and in such a manner that the first propagation route for the ultrasonic waves propagating from the first ultrasonic wave transmitter to the first ultrasonic wave receiver and the second propagation route for ultrasonic waves propagating from the second ultrasonic wave transmitter to the second ultrasonic wave receiver intersect each other and extend in opposite directions. The phase of the respective ultrasonic wave signals output by the first and second ultrasonic wave receivers are compared by a phase comparator so that the vortices generated by the vortex generator can be detected.
    • 一种包括超声波发射器和超声波接收器的涡街流量计,其适用于通过涡流发生器产生涡流的流体流接收由发射器发射的超声波,由超声波接收器输出的超声波信号被相位 比较器通过检测由于涡流的产生引起的超声波的相位差来测量流体的流速。 第一和第二超声波发射器设置在管线的壁上,其限定用于涡流发生器下游的流体的流动路径,并且第一和第二超声波接收器分别设置在与第一 以及第二发送器,并且使得从第一超声波发射器传播到第一超声波接收器的超声波的第一传播路径和从第二超声波发射器传播到第二超声波的超声波的第二传播路径 接收器彼此相交并在相反方向上延伸。 通过相位比较器比较由第一和第二超声波接收器输出的各超声波信号的相位,从而可以检测由涡流发生器产生的涡流。