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    • 3. 发明授权
    • Cloned genes coding for avian coccidiosis antigens which induce a
cell-mediated immune response
    • 编码诱导细胞介导的免疫应答的禽类球虫抗原的克隆基因
    • US5122471A
    • 1992-06-16
    • US308219
    • 1989-02-09
    • Mark C. JenkinsHyun S. LillehojJohn B. DameHarry D. DanforthMichael D. Ruff
    • Mark C. JenkinsHyun S. LillehojJohn B. DameHarry D. DanforthMichael D. Ruff
    • A61K39/00C07K14/455C07K16/20C12N1/21
    • C07K14/455C07K16/20A61K39/00
    • Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences.The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis.The DNA sequences of the present invention comprise cloned genes or fragments thereof that code on expression for an antigenic protein that activates white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein.
    • 公开了编码抗原蛋白质的DnA序列,鉴定此类DNA序列的方法和由该DNA序列编码的抗原。 该方法的第一步是提供多个DNA序列。 然后将这些序列插入DNA表达载体中以形成重组表达载体。 将表达载体插入合适的宿主中以形成表达DNA序列的转化体。 然后将转化体与针对艾美球虫抗原的抗体接触,以鉴定含有编码艾美球虫抗原的DNA序列的转化体。 然后从鉴定为编码抗原的DNA序列产生这些抗原。 这样产生的抗原与影响细胞介导的免疫应答的白细胞接触,白血球对抗原性艾美球虫蛋白致敏,从而鉴定编码诱导细胞介导的免疫应答的抗原的DNA序列 球虫病 本发明的DNA序列包含编​​码激活影响细胞介导的免疫应答的白细胞的抗原性蛋白质的表达的克隆基因或其片段,所述白细胞对抗原性艾美球虫蛋白致敏。