专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07030218B2 Pseudo native chemical ligation 失效
标题翻译：伪天然化学结扎
公开(公告)号：US07030218B2
公开(公告)日：2006-04-18
申请号：US10332386
申请日：2001-07-12
申请人： Christie L. Hunter , Paolo Botti , James A. Bradburne , Shiah-yun Chen , Sonya Cressman , Stephen B. H. Kent , Gerd G. Kochendoerfer , Donald W. Low
发明人： Christie L. Hunter , Paolo Botti , James A. Bradburne , Shiah-yun Chen , Sonya Cressman , Stephen B. H. Kent , Gerd G. Kochendoerfer , Donald W. Low
IPC分类号： C07K1/02 , C07K1/07
CPC分类号： C07K14/505 , A61K38/00
摘要： The present invention concerns methods and compositions for extending the technique of native chemical ligation of a wider range of peptides, polypeptides, other polymers and other molecules via an amide bond (see FIG. 1). The invention further provides methods and uses for such proteins and derivatized proteins. The invention is particularly suitable for use in the synthesis of optionally polymer-modified, synthetic bioactive proteins, and of pharmaceutical compositions that contain such proteins.
摘要翻译：本发明涉及通过酰胺键扩展更宽范围的肽，多肽，其它聚合物和其它分子的天然化学连接技术的方法和组合物（参见图1）。本发明还提供了这些蛋白质和衍生化蛋白质的方法和用途。本发明特别适用于合成任选的聚合物改性的合成生物活性蛋白质和含有这种蛋白质的药物组合物。

2. 发明授权

US06451543B1 Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides 有权
标题翻译：脂质基质辅助化学连接和膜多肽的合成
公开(公告)号：US06451543B1
公开(公告)日：2002-09-17
申请号：US09384302
申请日：1999-08-26
申请人： Gerd. G. Kochendoerfer , Christie L. Hunter , Stephen B. H. Kent , Paolo Botti
发明人： Gerd. G. Kochendoerfer , Christie L. Hunter , Stephen B. H. Kent , Paolo Botti
IPC分类号： G01N3353
CPC分类号： A61K49/0056 , A61K49/0041 , C07C229/16 , C07K1/023 , C07K1/026 , C07K1/04 , C07K1/042 , C07K1/045 , C07K14/005 , C07K14/215 , C07K14/36 , C07K14/43522 , C07K14/5421 , C07K14/705 , C07K2319/00 , C12N2740/16322 , C12N2760/16022 , Y10S435/87
摘要： The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.
摘要翻译：本发明涉及掺入脂质基质中的脂质基质辅助化学连接和膜多肽的合成的方法和组合物。本发明通过逐步化学选择性化学连接未受保护的肽段（其中至少一个肽片段嵌入脂质基质）生产嵌入脂质基质内的预折叠膜多肽。可以使用适用于未保护肽段连接的任何化学选择性反应化学物质。合适的脂质基质包括脂质体，胶束，细胞膜片和光学各向同性立体脂质相基质。根据本发明的方法和组合物合成的预先折叠的合成和半合成膜多肽还允许位点特异性引入一个或多个可检测部分，例如发色团，其可以在合成期间方便地引入。本发明的方法和组合物具有多种用途。例如，它们可用于测定配体结合膜多肽和包含受体的结构域，因此对于结构/功能研究，药物筛选/选择/设计和诊断等（包括高通量应用）非常有用。本发明的方法和组合物特别适用于以前不可接近的膜多肽的FRET分析。

3. 发明授权

US07482425B2 Compositions for lipid matrix-assisted chemical ligation 失效
标题翻译：用于脂质基质辅助化学连接的组合物
公开(公告)号：US07482425B2
公开(公告)日：2009-01-27
申请号：US10207330
申请日：2002-07-30
申请人： Gerd. G. Kochendoerfer , Christie L. Hunter , Stephen B. H. Kent , Paolo Botti
发明人： Gerd. G. Kochendoerfer , Christie L. Hunter , Stephen B. H. Kent , Paolo Botti
IPC分类号： A61K38/00
CPC分类号： C07K14/005 , C07K1/023 , C07K1/026 , C07K1/04 , C07K1/042 , C07K14/215 , C07K14/245 , C07K14/36 , C07K14/43522 , C07K14/5421 , C07K14/705 , C07K2319/00 , C12N2740/16322 , C12N2760/16022
摘要： The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.
摘要翻译：本发明涉及掺入脂质基质中的脂质基质辅助化学连接和膜多肽的合成的方法和组合物。本发明通过逐步化学选择性化学连接未受保护的肽段（其中至少一个肽片段嵌入脂质基质）生产嵌入脂质基质内的预折叠膜多肽。可以使用适用于未保护肽段连接的任何化学选择性反应化学物质。合适的脂质基质包括脂质体，胶束，细胞膜片和光学各向同性立体脂质相基质。根据本发明的方法和组合物合成的预先折叠的合成和半合成膜多肽还允许位点特异性引入一个或多个可检测部分，例如发色团，其可以在合成期间方便地引入。本发明的方法和组合物具有多种用途。例如，它们可用于测定配体结合膜多肽和包含受体的结构域，因此对于结构/功能研究，药物筛选/选择/设计和诊断等（包括高通量应用）非常有用。本发明的方法和组合物特别适用于以前不可接近的膜多肽的FRET分析。

4. 发明授权

US07118737B2 Polymer-modified synthetic proteins 失效
标题翻译：聚合物修饰的合成蛋白
公开(公告)号：US07118737B2
公开(公告)日：2006-10-10
申请号：US10332385
申请日：2001-07-12
申请人： Gerd G. Kochendoerfer , Paolo Botti , James A. Bradburne , Shiah-yun Chen , Sonya Cressman , Christie L. Hunter , Stephen B. H. Kent , Donald W. Low , Jill G. Wilken
发明人： Gerd G. Kochendoerfer , Paolo Botti , James A. Bradburne , Shiah-yun Chen , Sonya Cressman , Christie L. Hunter , Stephen B. H. Kent , Donald W. Low , Jill G. Wilken
IPC分类号： A61K38/16 , A61K38/17 , A61K39/19 , C07K1/113 , C07K14/00 , C07K14/435 , C07K14/52
CPC分类号： C07K14/52 , C07K1/1077 , C12P21/02
摘要： The present invention relates to methods and compositions for modifying peptides, polypeptides and proteins with polymers, especially glyco-mimetic polymers, so as to improve their biological activity or pharmacokinetic properties. The invention further provides methods and uses for such polymer-modified peptides, polypeptides and proteins. The invention is particularly suitable for use in the synthesis of polymer-modified synthetic bioactive proteins (FIG. 1D), and of pharmaceutical compositions that contain such proteins.
摘要翻译：本发明涉及用聚合物，特别是糖模拟聚合物修饰肽，多肽和蛋白质的方法和组合物，以改善其生物学活性或药代动力学性质。本发明进一步提供了这种聚合物修饰的肽，多肽和蛋白质的方法和用途。本发明特别适用于合成聚合物改性的合成生物活性蛋白（图1D）和含有这种蛋白质的药物组合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式