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1. 发明申请

US20090119787A1 CELL NUCLEAR TRANSFER 有权
标题翻译：细胞核转移
公开(公告)号：US20090119787A1
公开(公告)日：2009-05-07
申请号：US12066169
申请日：2006-09-08
申请人： Yutao Du , Lars Axel Bolund , Gabor Vajta , Peter Michael Kragh
发明人： Yutao Du , Lars Axel Bolund , Gabor Vajta , Peter Michael Kragh
IPC分类号： A01K67/027 , A01K67/00
CPC分类号： C12N15/877 , A01K2217/05 , C12N15/8778
摘要： The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.
摘要翻译：本发明公开了用于细胞核转移的方法，其包括例如修饰卵母细胞的透明带，和/或将卵母细胞分成若干部分。本发明还公开了用于生产遗传修饰的非人哺乳动物的方法。通过公开的方法可获得的转基因非人哺乳动物也在本发明的范围内。还公开了冷冻保存细胞的方法。

2. 发明授权

US06399375B2 Method and apparatus for culturing cells and tissues 失效
标题翻译：培养细胞和组织的方法和装置
公开(公告)号：US06399375B2
公开(公告)日：2002-06-04
申请号：US09341025
申请日：1999-07-15
申请人： Gabor Vajta
发明人： Gabor Vajta
IPC分类号： C12N500
CPC分类号： C12M21/06 , C12M23/14 , C12M41/22
摘要： Cells and tissues, in particular sensitive cells and tissues, such as oocytes, fertilized oocytes and preimplantation embryos, which require highly stable physical and chemical environment for in vitro development, are cultured in closed containers submerged or immersed in thermostatically controlled liquid baths, the containers being provided with an appropriate inner atmosphere containing, e.g. carbondioxide, oxygen and humidity in appropriate levels. The incubator containers may e.g. be gas and liquid impervious, flexible, sealable, preferably transparent bags which after sealing are submerged or immersed directly in the thermostatically controlled liquid bath. The liquid in the bath is preferably water. An incubator for submerse or immerse culture of cells and tissues in the above manner is also described. Also a transportable liquid incubator for culturing cells and tissues in the field is described.
摘要翻译：将需要高度稳定的物理和化学环境用于体外发育的细胞和组织，特别是敏感细胞和组织，例如卵母细胞，受精卵母细胞和植入前胚胎培养在浸没在或浸没在恒温控制液浴中的封闭容器中，容器提供适当的内部气氛，包含例如二氧化碳，氧气和湿度在适当的水平。培养箱容器可以例如。是气体和液体不可渗透的，柔性的，可密封的，优选的透明袋，其在密封之后被浸没或直接浸入恒温控制液浴中。浴中的液体优选为水。还描述了以上述方式浸泡或浸泡细胞和组织培养的培养箱。还描述了用于在田间培养细胞和组织的可运送液体培养箱。

3. 发明授权

US08513486B2 Cell nuclear transfer 有权
标题翻译：细胞核转移
公开(公告)号：US08513486B2
公开(公告)日：2013-08-20
申请号：US12066169
申请日：2006-09-08
申请人： Yutao Du , Lars Axel Bolund , Gabor Vajta , Peter Michael Kragh
发明人： Yutao Du , Lars Axel Bolund , Gabor Vajta , Peter Michael Kragh
IPC分类号： C12N15/00 , C12N15/02
CPC分类号： C12N15/877 , A01K2217/05 , C12N15/8778
摘要： The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.
摘要翻译：本发明公开了用于细胞核转移的方法，其包括例如修饰卵母细胞的透明带，和/或将卵母细胞分成若干部分。本发明还公开了用于生产遗传修饰的非人哺乳动物的方法。通过公开的方法可获得的转基因非人哺乳动物也在本发明的范围内。还公开了冷冻保存细胞的方法。

4. 发明申请

US20080092249A1 Method of nuclear transfer 审中-公开
标题翻译：核转移方法
公开(公告)号：US20080092249A1
公开(公告)日：2008-04-17
申请号：US11808383
申请日：2007-06-08
申请人： Ian Lewis , Gabor Vajta , Tayfur Tecirlioglu
发明人： Ian Lewis , Gabor Vajta , Tayfur Tecirlioglu
IPC分类号： A01K67/00 , C12N15/00
CPC分类号： C12N15/873
摘要： The present invention relates to nuclear methods and embryos developed therefrom. In particular, the present invention relates to a method of nuclear comprising the step of transferring a somatic cell nuclei into a zona pellucida-free, enucleated oocyte.
摘要翻译：本发明涉及从其开发的核方法和胚胎。特别地，本发明涉及核的方法，其包括将体细胞核转移到无透明的去核卵母细胞中的步骤。

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