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1. 发明申请

WO2004009540A2 METHODS AND KITS FOR TRANSFERASES 审中-公开
标题翻译：用于转移的方法和工具
公开(公告)号：WO2004009540A2
公开(公告)日：2004-01-29
申请号：PCT/US2003/022315
申请日：2003-07-17
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , BULLEIT, Robert, F.
发明人： GOUELI, Said, A. , BULLEIT, Robert, F.
IPC分类号： C07D
CPC分类号： G01N33/542 , C07K7/06 , C07K7/08 , C07K2319/60 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/485
摘要： A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
摘要翻译：用于检测样品的转移酶活性的方法包括使样品与底物和磷酸基供体和磷酸酯基受体中的至少一种接触。底物包括报告化合物和氨基酸。加入肽酶，其以第一速率和磷酸化底物和第二速率切割非磷酸化底物。检测报告化合物的输出。在优选的实施方案中，所检测的转移酶活性是激酶活性。在另一个优选的实施方案中，所检测的转移酶活性是磷酸酶活性。还提供了筛选转移酶反应中的改变的方法。还提供了用于实施本发明的至少一种方法的试剂盒和肽底物。

2. 发明申请

WO2010011607A1 ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY 审中-公开
标题翻译：基于ADP检测的LUMINESCENT PHOSPHOTRANSFERASE或ATP水解测定
公开(公告)号：WO2010011607A1
公开(公告)日：2010-01-28
申请号：PCT/US2009/051170
申请日：2009-07-20
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , ZEGZOUTI, Hicham
发明人： GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/008
摘要： The invention provides provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP.
摘要翻译：本发明提供了确定或检测酶的活性的组合物和方法，包括磷酸转移酶例如激酶（例如蛋白质，脂质和糖激酶）和ATP水解酶例如使用ATP作为底物的ATP酶，例如HSP90，通过监测ADP的变化，形成ADP作为产品。

3. 发明申请

WO2004009540A3 METHODS AND KITS FOR TRANSFERASES 审中-公开
标题翻译：转移方法和试剂盒
公开(公告)号：WO2004009540A3
公开(公告)日：2004-11-04
申请号：PCT/US0322315
申请日：2003-07-17
申请人： PROMEGA CORP , GOUELI SAID A , BULLEIT ROBERT F
发明人： GOUELI SAID A , BULLEIT ROBERT F
IPC分类号： C07K7/06 , C07K7/08 , C12Q1/37 , C12Q1/48 , G01N33/542 , C12N9/10 , C12N9/12 , C12N9/16 , C12Q1/42
CPC分类号： G01N33/542 , C07K7/06 , C07K7/08 , C07K2319/60 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/485
摘要： A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
摘要翻译：用于检测样品的转移酶活性的方法包括使样品与底物以及磷酸基供体和磷酸基受体中的至少一个接触。底物包括报道化合物和氨基酸。添加肽酶，其以第一速率裂解非磷酸化底物并且以第二速率裂解磷酸化底物。检测到报道化合物的输出。在一个优选的实施方案中，检测到的转移酶活性是激酶活性。在另一个优选的实施方案中，检测的转移酶活性是磷酸酶活性。还提供了筛选转移酶反应中的改变的方法。还提供试剂盒和肽底物用于实施本发明的至少一种方法。

4. 发明申请

WO2007067557A3 METHODS FOR CYCLIC NUCLEOTIDE DETERMINATION 审中-公开
标题翻译：循环核糖核酸测定方法
公开(公告)号：WO2007067557A3
公开(公告)日：2007-12-13
申请号：PCT/US2006046431
申请日：2006-12-06
申请人： PROMEGA CORP , GOUELI SAID A , HSAIO KUEI-HSUAN , KUMAR MEERA , VIDUGIRIENE JOLANTA
发明人： GOUELI SAID A , HSAIO KUEI-HSUAN , KUMAR MEERA , VIDUGIRIENE JOLANTA
IPC分类号： C07K16/00 , C12P21/08
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples.
摘要翻译：本发明一般涉及细胞分析工具，更具体地涉及用于检测或确定样品中环核苷酸浓度的方法。

5. 发明申请

WO2004009540A8 METHODS AND KITS FOR TRANSFERASES 审中-公开
标题翻译：用于转移的方法和工具
公开(公告)号：WO2004009540A8
公开(公告)日：2005-09-01
申请号：PCT/US0322315
申请日：2003-07-17
申请人： PROMEGA CORP , GOUELI SAID A , BULLEIT ROBERT F
发明人： GOUELI SAID A , BULLEIT ROBERT F
IPC分类号： C07K7/06 , C07K7/08 , C12Q1/37 , C12Q1/48 , G01N33/542 , C12N9/10 , C12N9/12 , C12N9/16 , C12Q1/42
CPC分类号： G01N33/542 , C07K7/06 , C07K7/08 , C07K2319/60 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/485
摘要： A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
摘要翻译：用于检测样品的转移酶活性的方法包括使样品与底物和磷酸基供体和磷酸酯基受体中的至少一种接触。底物包括报告化合物和氨基酸。加入肽酶，其以第一速率和磷酸化底物和第二速率切割非磷酸化底物。检测报告化合物的输出。在优选的实施方案中，所检测的转移酶活性是激酶活性。在另一个优选的实施方案中，所检测的转移酶活性是磷酸酶活性。还提供了筛选转移酶反应中的改变的方法。还提供了用于实施本发明的至少一种方法的试剂盒和肽底物。

6. 发明申请

WO2007067557A2 METHODS FOR CYCLIC NUCLEOTIDE DETERMINATION 审中-公开
标题翻译：循环核苷酸测定方法
公开(公告)号：WO2007067557A2
公开(公告)日：2007-06-14
申请号：PCT/US2006/046431
申请日：2006-12-06
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , HSAIO, Kuei-Hsuan , KUMAR, Meera , VIDUGIRIENE, Jolanta
发明人： GOUELI, Said, A. , HSAIO, Kuei-Hsuan , KUMAR, Meera , VIDUGIRIENE, Jolanta
IPC分类号： G01N33/53 , C12Q1/66 , C12Q1/48
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples.
摘要翻译：本发明一般涉及细胞分析工具，更具体地涉及用于检测或确定样品中环核苷酸浓度的方法。

7. 发明申请

WO2004023098A3 METHOD FOR DETECTING TRANSFERASE ENZYMATIC ACTIVITY 审中-公开
标题翻译：检测转移酶活性的方法
公开(公告)号：WO2004023098A3
公开(公告)日：2004-07-01
申请号：PCT/US0327854
申请日：2003-09-05
申请人： PROMEGA CORP , SOMBERG RICHARD , GOUELI SAID A
发明人： SOMBERG RICHARD , GOUELI SAID A
IPC分类号： C12P13/16 , C12Q1/48 , C12Q1/66 , G01N20060101 , G01N33/53 , C12N13/16
CPC分类号： C12Q1/66 , C12Q1/485
摘要： Methods and kits for detecting transferase activity in a sample by measuring ATP using a composition comprising an ATP-dependent bioluminescence-generating enzyme, a luminogenic molecule and one or more transferase quenching agents.
摘要翻译：通过使用包含ATP依赖性生物发光酶，发光分子和一种或多种转移酶猝灭剂的组合物测量ATP来检测样品中转移酶活性的方法和试剂盒。

8. 发明申请

WO2004023098A2 METHOD FOR DETECTING TRANSFERASE ENZYMATIC ACTIVITY 审中-公开
标题翻译：检测转移酶活性的方法
公开(公告)号：WO2004023098A2
公开(公告)日：2004-03-18
申请号：PCT/US2003/027854
申请日：2003-09-05
申请人： PROMEGA CORPORATION , SOMBERG, Richard , GOUELI, Said, A.
发明人： SOMBERG, Richard , GOUELI, Said, A.
IPC分类号： G01N
CPC分类号： C12Q1/66 , C12Q1/485
摘要： Methods and kits for detecting transferase activity in a sample by measuring ATP using a composition comprising an ATP-dependent bioluminescence-generating enzyme, a luminogenic molecule and one or more transferase quenching agents.
摘要翻译：通过使用包含ATP依赖性生物发光生成酶，发光分子和一种或多种转移酶猝灭剂的组合物测量ATP来检测样品中转移酶活性的方法和试剂盒。

9. 发明申请

WO1998022122A1 ALKYL PEPTIDE AMIDES AND APPLICATIONS 审中-公开
标题翻译：烷基肽类药物和应用
公开(公告)号：WO1998022122A1
公开(公告)日：1998-05-28
申请号：PCT/US1997021509
申请日：1997-11-20
申请人： PROMEGA CORPORATION , GOUELI, Said, A.
发明人： PROMEGA CORPORATION
IPC分类号： A61K38/00
CPC分类号： C07K14/47 , A61K38/00
摘要： The present invention is directed to an alkyl peptide amide, to pharmaceutical compositions and to a process for introducing at least the amide portion thereof into the intracellular space of living cells. The compositions and methods of the present invention can be used to introduce peptide or peptido-mimetic moieties, including anchor inhibiting peptide moieties, into the intracellular space of a variety of different types of cells, including sperm cells from various species.
摘要翻译：本发明涉及烷基肽酰胺，药物组合物和至少将其酰胺部分引入活细胞的细胞内空间的方法。本发明的组合物和方法可用于将肽或肽模拟部分（包括锚定抑制肽部分）引入各种不同类型细胞的细胞内空间，包括来自各种物种的精细胞。

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