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1. 发明申请

WO2010005525A1 POLYMERS OF ISOPRENE FROM RENEWABLE RESOURCES 审中-公开
标题翻译：可再生能源的ISOPRENE聚合物
公开(公告)号：WO2010005525A1
公开(公告)日：2010-01-14
申请号：PCT/US2009/003897
申请日：2009-06-30
申请人： THE GOODYEAR TIRE & RUBBER COMPANY , GENENCOR INTERNATIONAL, INC. , FEHER, Frank, J. , WHITED, Gregory, M. , CHOTANI, Gopal, K. , VALLE, Fernando , FIORESI, Carol , SANFORD, Karl, J. , MCAULIFFE, Joseph, C. , CERVIN, Marguerite , PUHALA, Aaron, S. , MIASNIKOV, Andrei , ALDOR, Illana, S.
发明人： FEHER, Frank, J. , WHITED, Gregory, M. , CHOTANI, Gopal, K. , VALLE, Fernando , FIORESI, Carol , SANFORD, Karl, J. , MCAULIFFE, Joseph, C. , CERVIN, Marguerite , PUHALA, Aaron, S. , MIASNIKOV, Andrei , ALDOR, Illana, S.
IPC分类号： C12P5/02 , C07C1/00
CPC分类号： C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
摘要： It has been found that certain cells in culture can convert carbon available in the cell culture medium into isoprene These cells have a heterologous nucleic acid which is operably linked to a promoter and encodes an isoprene synthase polypeptide The isoprene produced in such a cultured medium can then be recovered and polymenzed into synthetic rubbers and other useful polymeπc matenals The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being denved from non- petrochemical based resources They can also be analytically distinguished from rubbers that come from natural sources The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are denved from isoprene monomer, wherein the polyisoprene polymer has delta 13C value of greater than -22%o This type of polyisoprene can be a αs-1 A- polyisoprene homopolymer rubber.
摘要翻译：已经发现，培养物中的某些细胞可将细胞培养基中可获得的碳转化为异戊二烯。这些细胞具有与启动子可操作地连接并编码异戊二烯合酶多肽的异源核酸。然后可以在这样的培养基中产生的异戊二烯被回收和聚合成合成橡胶和其他有用的聚合物材料本发明的合成异戊二烯聚合物提供了可被验证为从非石化基础资源中被证实的益处。它们还可以与来自天然来源的橡胶进行分析区分。本发明更具体地公开了由异戊二烯单体所取代的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物具有大于-22％的δ13 C值。这种类型的聚异戊二烯可以是一种1-聚异戊二烯均聚物橡胶。

2. 发明申请

WO2017079756A1 PAENIBACILLUS AND BACILLUS SPP. MANNANASES 审中-公开
标题翻译： PAENIBACILLUS和BACILLUS SPP。甘露聚糖
公开(公告)号：WO2017079756A1
公开(公告)日：2017-05-11
申请号：PCT/US2016/060850
申请日：2016-11-07
申请人： DANISCO US INC , BAO, Kai , FIORESI, Carol Marie , FINAN, Dina , ADAMS, Christian D. , ALEKSEYEV, Viktor Yuryevich , BOTT, Richard R. , RIFE, Christopher L.
发明人： BAO, Kai , FIORESI, Carol Marie , FINAN, Dina , ADAMS, Christian D. , ALEKSEYEV, Viktor Yuryevich , BOTT, Richard R. , RIFE, Christopher L.
IPC分类号： C12N9/24 , C11D3/386 , C12P21/06 , A23J3/34
CPC分类号： C11D3/386 , C12N9/2488 , C12N9/2494 , C12Y302/01078
摘要： Disclosed herein are mannanases from Paenibacillus or Bacillus spp , polynucleotides encoding the mannanases, compositions containing the mannanases, and methods of use thereof. Compositions containing mannanases are suitable for use as detergents and for cleaning fabrics and hard surfaces, as well as in a variety of other industrial applications.
摘要翻译：本文公开了来自类芽孢杆菌属或芽孢杆菌属的甘露聚糖酶，编码甘露聚糖酶的多核苷酸，含有甘露聚糖酶的组合物及其使用方法。含有甘露聚糖酶的组合物适合用作洗涤剂和用于清洁织物和硬质表面以及用于各种其他工业应用。

3. 发明申请

WO2005007818A8 EXO-SPECIFIC AMYLASE POLYPEPTIDES, NUCLEIC ACIDS ENCODING THOSE POLYPEPTIDES AND USES THEREOF 审中-公开
标题翻译：超特异性氨基酸多肽，编码多肽的核酸及其用途
公开(公告)号：WO2005007818A8
公开(公告)日：2006-03-16
申请号：PCT/US2004021723
申请日：2004-07-07
申请人： GENENCOR INT , DANISCO , BERG CASPER TUNE , BOJSEN KIRSTEN , DERKX PATRICK M F , FIORESI CAROL , GERRITSE GIJSBERT , KRAGH KARSTEN M , LIU WEI , SHAW ANDREW , THOUDAHL CHARLOTTE R
发明人： BERG CASPER TUNE , BOJSEN KIRSTEN , DERKX PATRICK M F , FIORESI CAROL , GERRITSE GIJSBERT , KRAGH KARSTEN M , LIU WEI , SHAW ANDREW , THOUDAHL CHARLOTTE R
IPC分类号： A21D8/02 , A21D8/04 , A23L1/03 , A23L1/09 , A23L1/305 , C07H21/04 , C11D3/386 , C12N1/21 , C12N9/28 , C12N9/32 , C12N15/74 , C12N
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶表现出改变的促进作用。

4. 发明申请

WO2007007053A1 MODIFIED AMYLASE FROM PSEUDOMONAS SACCHAROPHILIA 审中-公开
标题翻译：来自PSEUDOMONAS SACCHAROPHILIA的修饰的AMYLASE
公开(公告)号：WO2007007053A1
公开(公告)日：2007-01-18
申请号：PCT/GB2006/002513
申请日：2006-07-07
申请人： DANISCO A/S , GENENCOR INTERNATIONAL, INC. , BERG, Casper, Tune , DERKX, Patrick, Maria, Franciscus , FIORESI, Carol , GERRITSE, Gijsbert , KELLET-SMITH, Anja, Hemmingen , KRAGH, Karsten, Matthias , LIU, Wei , SHAW, Andrew , SØRENSEN, Bo, Spange , THOUDAHL, Charlotte, Refdahl
发明人： BERG, Casper, Tune , DERKX, Patrick, Maria, Franciscus , FIORESI, Carol , GERRITSE, Gijsbert , KELLET-SMITH, Anja, Hemmingen , KRAGH, Karsten, Matthias , LIU, Wei , SHAW, Andrew , SØRENSEN, Bo, Spange , THOUDAHL, Charlotte, Refdahl
IPC分类号： C12N9/28
CPC分类号： A21D8/042 , A23L33/18 , C12N9/2411 , C12N9/2414 , C12N9/2417 , C12N9/2425 , C12N9/2428
摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1 , uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
摘要翻译：我们描述了可从具有选自以下的淀粉酶活性的亲本多肽衍生的PS4变体多肽：（a）包含位置33,34,121,134,141,146,157,161中的每一个的氨基酸突变的多肽，178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,177,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参考如SEQ ID NO：1所示的假单胞菌属（Saccharophilia）淀粉酶序列的位置编号，使用这样的多肽作为食品或饲料添加剂，以及编码其的核酸。

5. 发明申请

WO2006003461A2 POLYPEPTIDE 审中-公开
标题翻译：多肽
公开(公告)号：WO2006003461A2
公开(公告)日：2006-01-12
申请号：PCT/GB2005/002675
申请日：2005-07-07
申请人： DANISCO A/S , GENENCOR INTERNATIONAL, INC. , BERG, Casper, Tune , DERKX, Patrick, M., F. , FIORESI, Carol , GERRITSE, Gijsbert , KELLET-SMITH, Anja, Hemmingen , KRAGH, Karsten, Matthias , LIU, Wei , SHAW, Andrew , SØRENSEN, Bo, Spange , THOUDAHL, Charlotte, Refdahl
发明人： BERG, Casper, Tune , DERKX, Patrick, M., F. , FIORESI, Carol , GERRITSE, Gijsbert , KELLET-SMITH, Anja, Hemmingen , KRAGH, Karsten, Matthias , LIU, Wei , SHAW, Andrew , SØRENSEN, Bo, Spange , THOUDAHL, Charlotte, Refdahl
IPC分类号： C12N9/00
CPC分类号： C12Y302/0106 , A21D8/042 , A23K20/189 , A23L7/107 , A23L29/35 , A23V2002/00 , C12N9/2417 , A23V2300/21
摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译：我们描述了源自具有非麦芽糖外淀粉酶活性的亲本多肽的PS4变体多肽，其中PS4变体多肽在选自以下的一个或多个位置包含氨基酸突变：121 ，161,223,146,157,158,198,229,303,306,309,316,353,26,70,145,188,272,339，参考嗜糖假单胞菌（Pseudomonas saccharophilia）的位置编号如SEQ ID NO：1所示的外切淀粉酶序列。

6. 发明申请

WO2005007818A2 EXO-SPECIFIC AMYLASE POLYPEPTIDES, NUCLEIC ACIDS ENCODING THOSE POLYPEPTIDES AND USES THEREOF 审中-公开
标题翻译：超特异性氨基酸多肽，编码这些多聚核苷酸的核酸及其用途
公开(公告)号：WO2005007818A2
公开(公告)日：2005-01-27
申请号：PCT/US2004/021723
申请日：2004-07-07
申请人： GENENCOR INTERNATIONAL, INC. , DANISCO A/S , GERRITSE, Gijsbert , BERG, Casper, Tune , BOJSEN, Kirsten , DERKX, Patrick, M.,.F. , FIORESI, Carol , KRAGH, Karsten, M. , LIU, Wei , SHAW, Andrew , THOUDAHL, Charlotte, R.
发明人： BERG, Casper, Tune , BOJSEN, Kirsten , DERKX, Patrick, M.,.F. , FIORESI, Carol , KRAGH, Karsten, M. , LIU, Wei , SHAW, Andrew , THOUDAHL, Charlotte, R.
IPC分类号： C12N
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶表现出改变的促进作用。

7. 发明申请

WO2010123754A1 PROTEASES WITH MODIFIED PRO REGIONS 审中-公开
标题翻译：具有修改的PRO区域的PROTEASES
公开(公告)号：WO2010123754A1
公开(公告)日：2010-10-28
申请号：PCT/US2010/031269
申请日：2010-04-15
申请人： DANISCO US INC. , FERRARI, Eugenio , FIORESI, Carol , VAN KIMMENADE, Anita
发明人： FERRARI, Eugenio , FIORESI, Carol , VAN KIMMENADE, Anita
IPC分类号： C12N15/75 , C12N9/54
CPC分类号： C12N9/54 , C12N15/75
摘要： The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
摘要翻译：本发明提供了用于在细菌宿主细胞中生产成熟蛋白酶的方法和组合物。所述组合物包括编码经修饰的蛋白酶的修饰的多核苷酸，所述修饰的多核苷酸在所述亲区具有至少一个突变; 由修饰的多核苷酸编码的修饰的丝氨酸蛋白酶; 表达盒，DNA构建体和包含编码修饰的蛋白酶的修饰的多核苷酸的载体; 和用本发明的载体转化的细菌宿主细胞。所述方法包括用于增强细菌宿主细胞中成熟蛋白酶的产生的方法，例如芽孢杆菌宿主细胞。产生的蛋白酶可用于工业生产酶，适用于各种行业，包括但不限于清洁，动物饲料和纺织加工业。

8. 发明申请

WO2009076676A3 COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE 审中-公开
公开(公告)号：WO2009076676A3
公开(公告)日：2009-06-18
申请号：PCT/US2008/086869
申请日：2008-12-15
申请人： DANISCO US INC. , THE GOODYEAR TIRE & RUBBER COMPANY , CERVIN, Marguerite , WHITED, Gregory, M. , CHOTANI, Gopal, K. , VALLE, Fernando , FIORESI, Carol , SANFORD, Karl, J. , MCAULIFFE, Joseph, C. , FEHER, Frank, J. , PUHALA, Aaron, S. , MIASNIKOV, Andrei , ALDOR, Ilana, S.
发明人： CERVIN, Marguerite , WHITED, Gregory, M. , CHOTANI, Gopal, K. , VALLE, Fernando , FIORESI, Carol , SANFORD, Karl, J. , MCAULIFFE, Joseph, C. , FEHER, Frank, J. , PUHALA, Aaron, S. , MIASNIKOV, Andrei , ALDOR, Ilana, S.
IPC分类号： C12P5/02
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

9. 发明申请

WO2008141281A1 A MODIFIED SECRETION SYSTEM TO INCREASE EXPRESSION OF POLYPEPTIDES IN BACTERIA 审中-公开
标题翻译：增加细菌中多聚糖表达的改良分泌系统
公开(公告)号：WO2008141281A1
公开(公告)日：2008-11-20
申请号：PCT/US2008/063418
申请日：2008-05-12
申请人： DANISCO US INC., GENENCOR DIVISION , VAN KIMMENADE, Anita , FIORESI, Carol , PERES, Caroline , FERRARI, Eugenio
发明人： VAN KIMMENADE, Anita , FIORESI, Carol , PERES, Caroline , FERRARI, Eugenio
IPC分类号： C07K14/32 , C12N15/31 , C12N15/75 , C12P21/02
CPC分类号： C12N15/75
摘要： The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.
摘要翻译：本发明提供了改变宿主细胞中所需多肽的产生的方法。特别地，本发明提供了编码截短的SecG蛋白的多核苷酸，其能够促进由细菌宿主细胞如芽孢杆菌属物种分泌所需的蛋白酶，以及表达载体和含有多核苷酸的宿主细胞。

10. 发明申请

WO2006003461A3 POLYPEPTIDE 审中-公开
标题翻译：多肽
公开(公告)号：WO2006003461A3
公开(公告)日：2006-08-17
申请号：PCT/GB2005002675
申请日：2005-07-07
申请人： DANISCO , GENENCOR INT , BERG CASPER TUNE , DERKX PATRICK M F , FIORESI CAROL , GERRITSE GIJSBERT , KELLET-SMITH ANJA HEMMINGEN , KRAGH KARSTEN MATTHIAS , LIU WEI , SHAW ANDREW , SOERENSEN BO SPANGE , THOUDAHL CHARLOTTE REFDAHL
发明人： BERG CASPER TUNE , DERKX PATRICK M F , FIORESI CAROL , GERRITSE GIJSBERT , KELLET-SMITH ANJA HEMMINGEN , KRAGH KARSTEN MATTHIAS , LIU WEI , SHAW ANDREW , SOERENSEN BO SPANGE , THOUDAHL CHARLOTTE REFDAHL
IPC分类号： C12N9/28 , A21D8/04 , A23K1/165 , A23L1/09 , C12P19/14
CPC分类号： C12Y302/0106 , A21D8/042 , A23K20/189 , A23L7/107 , A23L29/35 , A23V2002/00 , C12N9/2417 , A23V2300/21
摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译：我们描述了可从具有非麦芽糖苷外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽在选自以下的一个或多个位置包含氨基酸突变：121,161,223,146,157 ，158,198,229,303,306,309,316,353,26,70,205,188,272,339，参考SEQ ID NO：1所示的假单胞菌属的嗜酸芽胞弧菌外切酶序列的位置编号。

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