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1. 发明申请

WO2010150213A1 PROTEIN 审中-公开
标题翻译：蛋白
公开(公告)号：WO2010150213A1
公开(公告)日：2010-12-29
申请号：PCT/IB2010/052868
申请日：2010-06-23
申请人： DANISCO A/S , MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
发明人： MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/18 , C12N9/20 , A23L1/015 , C12N15/55
CPC分类号： C12N9/20 , A21D8/042 , C12N9/18 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032
摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.
摘要翻译：本发明涉及一种制备变体脂肪分解酶的方法，其包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包括在对应的位置处的至少一个修饰在编码的氨基酸序列中a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点（或另外一个糖基化位点）; b）在多肽的表面位置和远离更亲水的酶（与原始氨基酸相比）的活性位点（催化三单元组）的外部环路的位置处引入至少一个氨基酸; 或c）在位置33,63,78,190,305,306或320中的一个或多个处的取代或插入或在一个或多个位置311-312或307-319处的缺失，其中每个氨基酸位置对应于当与SEQ ID No.2对齐时，氨基酸序列的位置; 其中当所述核苷酸序列与编码SEQ ID No.22或SEQ ID No.23所示的真菌脂肪分解酶的核苷酸序列具有至少90％同一性时，所述修饰不是位置63处的取代，并且缺失不在位置311 -312。优选地，核苷酸序列与SEQ ID No.1具有至少90％的同一性。本发明还涉及通过该方法产生的多肽和新的核酸。

2. 发明申请

WO2010124146A2 THREE-DIMENSIONAL STRUCTURE OF ISOPRENE SYNTHASE AND ITS USE THEREOF FOR GENERATING VARIANTS 审中-公开
标题翻译：异丙肾上腺素的三维结构及其用于产生变异体
公开(公告)号：WO2010124146A2
公开(公告)日：2010-10-28
申请号：PCT/US2010/032134
申请日：2010-04-22
申请人： DANISCO US INC. , THE GOODYEAR TIRE & RUBBER COMPANY , BECK, Zachary, Q. , BOTT, Richard, R. , RIFE, Christopher, Lee , WELLS, Derek, H.
发明人： BECK, Zachary, Q. , BOTT, Richard, R. , RIFE, Christopher, Lee , WELLS, Derek, H.
IPC分类号： C12N9/88
CPC分类号： C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
摘要： The present invention provides a three-dimensional structures of P . tremuloides isoprene synthase and P . alba isoprene synthase. The invention also provides methods of using the three dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。本发明还提供了使用三维结构来设计异戊二烯合酶的方法，所述异戊二烯合酶具有改善的微生物宿主细胞中异戊二烯生产增加的活性。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

3. 发明申请

WO2009002480A9 ACYL TRANSFERASE HAVING ALTERED SUBSTRATE SPECIFICITY 审中-公开
标题翻译： ACYL转移酶具有改变的基质特异性
公开(公告)号：WO2009002480A9
公开(公告)日：2009-04-09
申请号：PCT/US2008007807
申请日：2008-06-23
申请人： DANISCO US INC GENENCOR DIV , BOTT RICHARD R , CERVIN MARGUERITE A
发明人： BOTT RICHARD R , CERVIN MARGUERITE A
IPC分类号： C07K14/435
CPC分类号： C12N9/1029
摘要： The present invention provides methods for engineering the M. smegmatis acyltransferase to create compositions comprising at least one enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis. The present invention further provides compositions comprising at least one perhydrolase enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis.
摘要翻译：本发明提供了用于工程化耻垢分枝杆菌酰基转移酶以产生包含至少一种适用于酶水性酰化和/或过水解的酶的组合物的方法。本发明进一步提供了包含至少一种适用于酶水性酰化和/或过水解的过水解酶的组合物。

4. 发明申请

WO2005056782A2 PERHYDROLASE 审中-公开
标题翻译：水解酶
公开(公告)号：WO2005056782A2
公开(公告)日：2005-06-23
申请号：PCT/US2004/040438
申请日：2004-12-03
申请人： GENENCOR INTERNATIONAL, INC. , THE PROCTER & GAMBLE COMPANY , AMIN, Neelam, S. , BOSTON, Matthew, G. , BOTT, Richard, R. , CERVIN, Marguerite, A. , CONCAR, Edward, M. , GUSTWILLER, Marc, E. , JONES, Brian, Edward , LIEBETON, Klaus , MIRACLE, Gregory, S. , OH, Hiroshi , POULOSE, Ayrookaran, J. , RAMER, Sandra, W. , SCHEIBEL, Jeffrey, J. , WEYLER, Walter , WHITED, Gregory M.
发明人： AMIN, Neelam, S. , BOSTON, Matthew, G. , BOTT, Richard, R. , CERVIN, Marguerite, A. , CONCAR, Edward, M. , GUSTWILLER, Marc, E. , JONES, Brian, Edward , LIEBETON, Klaus , MIRACLE, Gregory, S. , OH, Hiroshi , POULOSE, Ayrookaran, J. , RAMER, Sandra, W. , SCHEIBEL, Jeffrey, J. , WEYLER, Walter , WHITED, Gregory M.
IPC分类号： C12N9/00
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译：本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。本发明特别用于涉及清洗，漂白和消毒的应用中。

5. 发明申请

WO2003101404A2 PREPARATIONS FOR TOPICAL SKIN USE AND TREATMENT 审中-公开
标题翻译：用于局部皮肤使用和治疗的准备
公开(公告)号：WO2003101404A2
公开(公告)日：2003-12-11
申请号：PCT/US2003/007411
申请日：2003-03-11
申请人： DOW CORNING CORPORATION , GENENCOR INTERNATIONAL, INC. , BOTT, Richard, R. , GEBERT, Mark, S. , KLYKKEN, Paal, Christian , MAZEAUD, Isabelle , THOMAS, Xavier, Jean-Paul
发明人： BOTT, Richard, R. , GEBERT, Mark, S. , KLYKKEN, Paal, Christian , MAZEAUD, Isabelle , THOMAS, Xavier, Jean-Paul
IPC分类号： A61K
CPC分类号： A61K47/24 , A61K9/0014 , A61K9/7007 , A61K9/7069 , A61K38/465 , A61K38/482 , A61L26/0019 , A61L26/0066 , A61L2300/254 , A61L2300/602 , A61L2300/622 , C08L83/04
摘要： The present invention is related to topical preparations for release of an active agent and to methods of making and using the topical preparations. The preparations may have an internal phase dispersed within an external phase. The internal phase may be a hydrophilic carrier and an active agent. The external phase may be a silicone matrix. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that is will not be used to interpret or limit the scope or meaning of the claims.
摘要翻译：本发明涉及用于释放活性剂的局部制剂以及制备和使用局部制剂的方法。制剂可能具有分散在外部相中的内相。内相可以是亲水载体和活性剂。外部相可以是硅树脂基质。要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。提交的理解是不会用于解释或限制权利要求的范围或含义。

6. 发明申请

WO0064485A8 SPECIFICALLY TARGETED CATALYTIC ANTAGONISTS AND USES THEREOF 审中-公开
标题翻译：特异性靶向催化拮抗剂及其用途
公开(公告)号：WO0064485A8
公开(公告)日：2001-02-08
申请号：PCT/US0010988
申请日：2000-04-21
申请人： GENENCOR INT , DAVIS BENJAMIN G , JONES JOHN BRYAN , BOTT RICHARD R , SANFORD KARL JOHN , ESTELL DAVID AARON
发明人： DAVIS BENJAMIN G , JONES JOHN BRYAN , BOTT RICHARD R , SANFORD KARL JOHN , ESTELL DAVID AARON
IPC分类号： C12N9/50 , A45C15/06 , A61K38/43 , A61K38/55 , A61K45/00 , A61K47/48 , A61P3/06 , A61P3/10 , A61P5/26 , A61P5/28 , A61P5/30 , A61P7/02 , A61P9/06 , A61P9/10 , A61P9/12 , A61P11/02 , A61P11/08 , A61P21/02 , A61P25/04 , A61P25/08 , A61P25/16 , A61P25/18 , A61P25/24 , A61P29/00 , A61P31/12 , A61P43/00 , C12N9/54 , G08B5/00
CPC分类号： B82Y5/00 , A61K47/51 , A61K47/665 , A61K47/6898
摘要： The invention provides chimeric molecules that are catalytic antagonists of a target molecule. The catalytic antagonists of this invention preferably comprise a targeting moiety attached to an enzyme that degrades the molecule specifically bound by the targeting moiety. The catalytic antagonists of this invention thus bind to a target recognized by the targeting moiety (e.g., a receptor) the enzyme component of the chimera then degrades all or part of the target. This typically results in a reduction or loss of activity of the target and release of the chimeric molecule. The chimeric molecule is then free to attack and degrade another target molecule.
摘要翻译：本发明提供作为靶分子的催化拮抗剂的嵌合分子。本发明的催化拮抗剂优选包含与降解由靶向部分特异性结合的分子的酶连接的靶向部分。因此，本发明的催化拮抗剂与靶向部分识别的靶（例如受体）结合，嵌合体的酶组分然后降解靶的全部或部分。这通常导致靶的活性降低或丧失，并释放嵌合分子。然后嵌合分子可以自由地攻击和降解另一个靶分子。

7. 发明申请

WO2011130222A2 COMPOSITIONS AND METHODS COMPRISING VARIANT PROTEASES 审中-公开
标题翻译：包含变种蛋白质的组合物和方法
公开(公告)号：WO2011130222A2
公开(公告)日：2011-10-20
申请号：PCT/US2011032050
申请日：2011-04-12
申请人： DANISCO US INC , BOTT RICHARD R , CASCAO-PEREIRA LUIS G , ESTELL DAVID A , GOEDEGEBUUR FRITS , POULOSE AYROOKARAN JOSEPH
发明人： BOTT RICHARD R , CASCAO-PEREIRA LUIS G , ESTELL DAVID A , GOEDEGEBUUR FRITS , POULOSE AYROOKARAN JOSEPH
IPC分类号： C12N9/56
CPC分类号： C12N9/54
摘要： The present invention provides variant proteases, compositions comprising such variant proteases, and methods of cleaning comprising such variant proteases.
摘要翻译：本发明提供了变体蛋白酶，包含这种变体蛋白酶的组合物和包含这种变体蛋白酶的清洁方法。

8. 发明申请

WO2009132220A9 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于改善微生物生产异烟肼的异丙肾上腺素变异体
公开(公告)号：WO2009132220A9
公开(公告)日：2010-05-06
申请号：PCT/US2009041581
申请日：2009-04-23
申请人： DANISCO US INC , GOODYEAR TIRE & RUBBER , CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R , RIFE CHRISTOPHER LEE
发明人： CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R , RIFE CHRISTOPHER LEE
IPC分类号： C12N9/88 , C12N5/10 , C12N15/52
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

9. 发明申请

WO2009132220A2 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于改善微生物生产异烟肼的异丙肾上腺素变异体
公开(公告)号：WO2009132220A2
公开(公告)日：2009-10-29
申请号：PCT/US2009/041581
申请日：2009-04-23
申请人： DANISCO US INC. , THE GOODYEAR TIRE & RUBBER COMPANY , CERVIN, Marguerite, A. , WHITED, Gregory, M. , MIASNIKOV, Andrei , PERES, Caroline, M. , WEYLER, Walter , WELLS, Derek, H. , BOTT, Richard, R.
发明人： CERVIN, Marguerite, A. , WHITED, Gregory, M. , MIASNIKOV, Andrei , PERES, Caroline, M. , WEYLER, Walter , WELLS, Derek, H. , BOTT, Richard, R.
IPC分类号： C12N9/88 , C12N15/52 , C12N5/10
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供包含至少一种具有改进的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

10. 发明申请

WO2009002480A3 ACYL TRANSFERASE HAVING ALTERED SUBSTRATE SPECIFICITY 审中-公开
标题翻译：具有改变基质特性的ACYL转移
公开(公告)号：WO2009002480A3
公开(公告)日：2009-02-19
申请号：PCT/US2008007807
申请日：2008-06-23
申请人： DANISCO US INC GENENCOR DIV , BOTT RICHARD R , CERVIN MARGUERITE A
发明人： BOTT RICHARD R , CERVIN MARGUERITE A
IPC分类号： C07K14/435
CPC分类号： C12N9/1029
摘要： The present invention provides methods for engineering the M. smegmatis acyltransferase to create compositions comprising at least one enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis. The present invention further provides compositions comprising at least one perhydrolase enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis.
摘要翻译：本发明提供了用于工程化耻垢分枝杆菌酰基转移酶以产生包含至少一种适用于酶水解酰化和/或过水解的酶的组合物的方法。本发明还提供了包含至少一种适用于酶水解酰化和/或过水解的过水解酶的组合物。

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