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1. 发明公开

EP3607090A1 HIGH PRECISION MICROPURIFICATION SYSTEM AND METHODS OF USE 审中-公开
公开(公告)号：EP3607090A1
公开(公告)日：2020-02-12
申请号：EP18781399.3
申请日：2018-04-06
申请人： Emmert-Buck, Michael R. , Tangrea, Michael Anthony
发明人： Emmert-Buck, Michael R. , Tangrea, Michael Anthony
IPC分类号： C12Q1/68

2. 发明专利

AU2018249596B2 High precision micropurification system and methods of use 未知
公开(公告)号：AU2018249596B2
公开(公告)日：2022-01-27
申请号：AU2018249596
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

3. 发明专利

CA3058904A1 HIGH PRECISION MICROPURIFICATION SYSTEM AND METHODS OF USE 未知
公开(公告)号：CA3058904A1
公开(公告)日：2018-10-11
申请号：CA3058904
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

4. 发明专利

AU2003253582A1 BIODETECTION METHOD 未知
公开(公告)号：AU2003253582A1
公开(公告)日：2003-12-22
申请号：AU2003253582
申请日：2003-03-20
申请人： EMMERT BUCK MICHAEL R
发明人： EMMERT-BUCK MICHAEL R
IPC分类号： G01N33/532 , G01N33/542 , G01N33/58 , C07H21/02 , C12Q1/70 , C07H21/04 , G01N33/53
摘要： A new and useful biodetection method for identifying a target biomolecule within a sample is provided which permits the accurate measurement of DNA, mRNA or protein in a complex biological sample. In particular, the inventive biodetection method provides a new method for identifying a target biomolecule in a sample in which the target biomolecule is present in low abundance relative to one or more non-target biomolecules, or when the target biomolecule and a non-target biomolecule have a shared epitope or nucleic acid sequence. The biodetection method utilizes a combination of at least one target probe and a competitor molecule in order to identify a target biomolecule in a sample. When the sample contains the target biomolecule and a non-target biomolecule having a shared epitope or nucleic acid sequence, two target probes are utilized in combination with a competitor molecule. A detectable signal is produced when the at least one target probe is in high concentration relative to the competitor molecule, thereby identifying the target biomolecule. If the competitor molecule is in high concentration in the vicinity of the at least one target probe, then a detectable signal is not produced.

5. 发明专利

AU2018249596A1 High precision micropurification system and methods of use 未知
公开(公告)号：AU2018249596A1
公开(公告)日：2019-10-31
申请号：AU2018249596
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

6. 发明专利

AU2003278535A1 HISTOSCREEN METHOD 未知
公开(公告)号：AU2003278535A1
公开(公告)日：2004-01-06
申请号：AU2003278535
申请日：2003-04-24
申请人： EMMERT BUCK MICHAEL R
发明人： EMMERT-BUCK MICHAEL R
IPC分类号： G01N1/28 , G01N33/569 , G01N33/53 , G01N33/543
摘要： A method for analyzing biomolecules, such as DNA, mRNA and proteins, in tissue samples, including tissue samples in which these biomolecules are expressed at moderate or low levels of abundance. The method allows multiple tissue samples to be analyzed simultaneously and can be utilized for fresh/frozen tissue samples as well as archival formalin-fixed, paraffin-embedded tissue specimens. The method comprises determining the cellular content of a tissue block, obtaining a plurality of small tissue sections from the tissue block in such a manner that each of said plurality of small tissue section contains at least one cell type, homogenizing each of said plurality of tissue sections in a buffer to create a homogenized specimen, applying said homogenized specimen onto a membrane in order to produce a HistoStamp, applying a labeled probe that is specific for an individual biomolecule corresponding to said at least one cell type to said HistoStamp, and measuring the signal from said labeled probe to determine the abundance level of said biomolecule.

7. 发明申请

WO2004080579A3 LIQUID TISSUE PREPARATION FROM HISTOPATOLOGICALLY PROCESSED BIOLOGICAL SAMPLES, TISSUES AND CELLS 审中-公开
标题翻译：来自生物化学生物样品，组织和细胞的液体组织制备
公开(公告)号：WO2004080579A3
公开(公告)日：2005-03-31
申请号：PCT/US2004007142
申请日：2004-03-10
申请人： EXPRESSION PATHOLOGY INC , EMMERT-BUCK MICHAEL R , DARFLER MARLENE M , KRIZMAN DAVID B
发明人： EMMERT-BUCK MICHAEL R , DARFLER MARLENE M , KRIZMAN DAVID B
IPC分类号： B01F20060101 , B01F1/00 , C07K16/00 , C12N15/00 , C12P21/04 , G01N1/30 , G01N1/40 , G01N1/44 , G01N33/53 , G01N33/574
CPC分类号： G01N1/4044 , C12N15/1003 , C12Q1/37 , G01N1/30 , G01N1/44
摘要： The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.
摘要翻译：本发明提供了将组织病理学处理的生物样品，组织和细胞直接转化成多用途生物分子裂解物的方法。该方法允许同时提取，分离，溶解和储存包含在组织病理学处理的生物样品中的所有生物分子，从而形成所述样品的代表性文库。这种多用途生物分子裂解物是可稀释的，可溶的，能够分级，并用于任何数量的后续实验。

8. 发明申请

WO2004080579A2 LIQUID TISSUE PREPARATION FROM HISTOPATOLOGICALLY PROCESSED BIOLOGICAL SAMPLES, TISSUES AND CELLS 审中-公开
标题翻译：来自生物化学生物样品，组织和细胞的液体组织制备
公开(公告)号：WO2004080579A2
公开(公告)日：2004-09-23
申请号：PCT/US2004/007142
申请日：2004-03-10
申请人： EXPRESSION PATHOLOGY, INC. , EMMERT-BUCK, Michael, R. , DARFLER, Marlene, M. , KRIZMAN, David, B.
发明人： EMMERT-BUCK, Michael, R. , DARFLER, Marlene, M. , KRIZMAN, David, B.
IPC分类号： B01F
CPC分类号： G01N1/4044 , C12N15/1003 , C12Q1/37 , G01N1/30 , G01N1/44
摘要： The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.
摘要翻译：本发明提供了将组织病理学处理的生物样品，组织和细胞直接转化成多用途生物分子裂解物的方法。该方法允许同时提取，分离，溶解和储存包含在组织病理学处理的生物样品中的所有生物分子，从而形成所述样品的代表性文库。这种多用途生物分子裂解物是可稀释的，可溶的，能够分级，并用于任何数量的后续实验。

9. 发明申请

WO2003066878A3 NUCLEIC ACID ANALYSIS USING LAYERED EXPRESSION SCANNING 审中-公开
公开(公告)号：WO2003066878A3
公开(公告)日：2003-08-14
申请号：PCT/US2003/002933
申请日：2003-01-31
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES , 20/20 GENE SYSTEMS, INC. , EMMERT-BUCK, Michael, R. , CHUAQUI, Rodrigo , FLAIG, Michael, J. , KNEZEVIC, Vladmir
发明人： EMMERT-BUCK, Michael, R. , CHUAQUI, Rodrigo , FLAIG, Michael, J. , KNEZEVIC, Vladmir
IPC分类号： C12Q1/68
摘要： Provided herein are methods of producing duplicates of nucleic acid samples, such as Northern blots, Southern blots, and sequencing gels, and methods of analyzing nucleic acids on such duplicates. Also provided are kits for using in producing duplicates of nucleic acid samples, and reagents for use in these kits and methods.

10. 发明申请

WO2010088517A8 METHODS AND SYSTEMS FOR PURIFYING TRANSFERRING AND/OR MANIPULATING NUCLEIC ACIDS 审中-公开
标题翻译：用于净化转运和/或操作核酸的方法和系统
公开(公告)号：WO2010088517A8
公开(公告)日：2011-07-28
申请号：PCT/US2010022586
申请日：2010-01-29
申请人： US HEALTH , UNIV MARYLAND , EMMERT-BUCK MICHAEL R , ARMANI MICHAEL , SMELA ELISABETH , SHAPIRO BENJAMIN , TANGREA MICHAEL A , RODRIGUEZ-CANALES JAIME , CHUAQUI RODRIGO , GILLESPIE JOHN
发明人： EMMERT-BUCK MICHAEL R , ARMANI MICHAEL , SMELA ELISABETH , SHAPIRO BENJAMIN , TANGREA MICHAEL A , RODRIGUEZ-CANALES JAIME , CHUAQUI RODRIGO , GILLESPIE JOHN
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , B01L3/5025 , B01L3/50853 , B01L3/50857 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0487 , C12N15/1006 , C12Q2565/631 , C12Q2565/513 , C12Q2547/101 , C12Q2523/125
摘要： The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information.
摘要翻译：本公开提供用于纯化，转移或操纵核酸的方法，系统和装置，同时保持核酸的2D空间关系，因为它们存在于具有2D空间信息的原始样品中。

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