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1. 发明专利

CA3058904A1 HIGH PRECISION MICROPURIFICATION SYSTEM AND METHODS OF USE 未知
公开(公告)号：CA3058904A1
公开(公告)日：2018-10-11
申请号：CA3058904
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

2. 发明专利

AU2018249596A1 High precision micropurification system and methods of use 未知
公开(公告)号：AU2018249596A1
公开(公告)日：2019-10-31
申请号：AU2018249596
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

3. 发明专利

AU2018249596B2 High precision micropurification system and methods of use 未知
公开(公告)号：AU2018249596B2
公开(公告)日：2022-01-27
申请号：AU2018249596
申请日：2018-04-06
申请人： EMMERT BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
发明人： EMMERT-BUCK MICHAEL R , TANGREA MICHAEL ANTHONY
IPC分类号： C12Q1/68
摘要： Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.

4. 发明申请

WO2004080579A3 LIQUID TISSUE PREPARATION FROM HISTOPATOLOGICALLY PROCESSED BIOLOGICAL SAMPLES, TISSUES AND CELLS 审中-公开
标题翻译：来自生物化学生物样品，组织和细胞的液体组织制备
公开(公告)号：WO2004080579A3
公开(公告)日：2005-03-31
申请号：PCT/US2004007142
申请日：2004-03-10
申请人： EXPRESSION PATHOLOGY INC , EMMERT-BUCK MICHAEL R , DARFLER MARLENE M , KRIZMAN DAVID B
发明人： EMMERT-BUCK MICHAEL R , DARFLER MARLENE M , KRIZMAN DAVID B
IPC分类号： B01F20060101 , B01F1/00 , C07K16/00 , C12N15/00 , C12P21/04 , G01N1/30 , G01N1/40 , G01N1/44 , G01N33/53 , G01N33/574
CPC分类号： G01N1/4044 , C12N15/1003 , C12Q1/37 , G01N1/30 , G01N1/44
摘要： The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.
摘要翻译：本发明提供了将组织病理学处理的生物样品，组织和细胞直接转化成多用途生物分子裂解物的方法。该方法允许同时提取，分离，溶解和储存包含在组织病理学处理的生物样品中的所有生物分子，从而形成所述样品的代表性文库。这种多用途生物分子裂解物是可稀释的，可溶的，能够分级，并用于任何数量的后续实验。

5. 发明专利

AU2003253582A1 BIODETECTION METHOD 未知
公开(公告)号：AU2003253582A1
公开(公告)日：2003-12-22
申请号：AU2003253582
申请日：2003-03-20
申请人： EMMERT BUCK MICHAEL R
发明人： EMMERT-BUCK MICHAEL R
IPC分类号： G01N33/532 , G01N33/542 , G01N33/58 , C07H21/02 , C12Q1/70 , C07H21/04 , G01N33/53
摘要： A new and useful biodetection method for identifying a target biomolecule within a sample is provided which permits the accurate measurement of DNA, mRNA or protein in a complex biological sample. In particular, the inventive biodetection method provides a new method for identifying a target biomolecule in a sample in which the target biomolecule is present in low abundance relative to one or more non-target biomolecules, or when the target biomolecule and a non-target biomolecule have a shared epitope or nucleic acid sequence. The biodetection method utilizes a combination of at least one target probe and a competitor molecule in order to identify a target biomolecule in a sample. When the sample contains the target biomolecule and a non-target biomolecule having a shared epitope or nucleic acid sequence, two target probes are utilized in combination with a competitor molecule. A detectable signal is produced when the at least one target probe is in high concentration relative to the competitor molecule, thereby identifying the target biomolecule. If the competitor molecule is in high concentration in the vicinity of the at least one target probe, then a detectable signal is not produced.

6. 发明专利

AU2003278535A1 HISTOSCREEN METHOD 未知
公开(公告)号：AU2003278535A1
公开(公告)日：2004-01-06
申请号：AU2003278535
申请日：2003-04-24
申请人： EMMERT BUCK MICHAEL R
发明人： EMMERT-BUCK MICHAEL R
IPC分类号： G01N1/28 , G01N33/569 , G01N33/53 , G01N33/543
摘要： A method for analyzing biomolecules, such as DNA, mRNA and proteins, in tissue samples, including tissue samples in which these biomolecules are expressed at moderate or low levels of abundance. The method allows multiple tissue samples to be analyzed simultaneously and can be utilized for fresh/frozen tissue samples as well as archival formalin-fixed, paraffin-embedded tissue specimens. The method comprises determining the cellular content of a tissue block, obtaining a plurality of small tissue sections from the tissue block in such a manner that each of said plurality of small tissue section contains at least one cell type, homogenizing each of said plurality of tissue sections in a buffer to create a homogenized specimen, applying said homogenized specimen onto a membrane in order to produce a HistoStamp, applying a labeled probe that is specific for an individual biomolecule corresponding to said at least one cell type to said HistoStamp, and measuring the signal from said labeled probe to determine the abundance level of said biomolecule.

7. 发明申请

WO03066878A2 NUCLEIC ACID ANALYSIS USING LAYERED EXPRESSION SCANNING 审中-公开
标题翻译：使用层状表达扫描的核酸分析
公开(公告)号：WO03066878A2
公开(公告)日：2003-08-14
申请号：PCT/US0302933
申请日：2003-01-31
申请人： US GOV HEALTH & HUMAN SERV , EMMERT-BUCK MICHAEL R , CHUAQUI RODRIGO , FLAIG MICHAEL J , KNEZEVIC VLADMIR , 20 20 GENE SYSTEMS INC
发明人： EMMERT-BUCK MICHAEL R , CHUAQUI RODRIGO , FLAIG MICHAEL J , KNEZEVIC VLADMIR
IPC分类号： B01L3/00 , C07H19/00 , C07H21/02 , C07H21/04 , C12Q20060101 , C12Q1/68 , C12Q
CPC分类号： B01L3/5023 , B01L2300/0636 , B01L2300/0874 , B01L2300/0887 , B01L2400/0406
摘要： Provided herein are methods of producing duplicates of nucleic acid samples, such as Northern blots, Southern blots, and sequencing gels, and methods of analyzing nucleic acids on such duplicates. Also provided are kits for using in producing duplicates of nucleic acid samples, and reagents for use in these kits and methods.
摘要翻译：本文提供了生产核酸样品重复的方法，例如Northern印迹，Southern印迹和测序凝胶，以及在这些重复物上分析核酸的方法。还提供了用于生产核酸样品的重复的试剂盒和用于这些试剂盒和方法的试剂。

8. 发明申请

WO0107915A2 METHOD AND DEVICE FOR ANALYSIS OF BIOLOGICAL SPECIMENS 审中-公开
标题翻译：用于分析生物样本的方法和装置
公开(公告)号：WO0107915A2
公开(公告)日：2001-02-01
申请号：PCT/US0020354
申请日：2000-07-26
申请人： US HEALTH , EMMERT BUCK MICHAEL R
发明人： EMMERT-BUCK MICHAEL R
IPC分类号： G01N33/48 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/02 , C12Q1/68 , C40B30/04 , G01N27/447 , G01N33/483 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/574 , G01N33/68
CPC分类号： G01N33/6803 , C12Q1/6841 , C40B30/04 , G01N33/54386 , G01N33/57434 , G01N33/6845 , G01N2333/4712 , G01N2333/4742 , G01N2333/7051 , G01N2333/96455 , G01N2333/96486
摘要： Disclosed herein are substrates having different capture regions, such as contiguous layers, wherein the different capture regions of the substrate contain different identification molecules. Components of the specimen are transfered through the capture regions under conditions which allow the components to interact with different identification molecules in the different regions of the substrate. Transfer is effected by capillary action of a solution moving through the cellular specimen or by electrophoresis. The transfer may occur in such a way as to maintain a geometric correspondence to the specimen, e.g. a correspondence to the cytoarchitecture of a cellular specimen. Example of cellular specimens include tissue sections such as tumor tissue sections. Cytostat sections cut slightly thicker than usual, i.e. 25 to 50 mu m, allow the detection of molecules of moderate and low level abundance.
摘要翻译：本文公开了具有不同捕获区域（例如连续层）的底物，其中底物的不同捕获区域含有不同的鉴定分子。在允许组分与衬底的不同区域中的不同识别分子相互作用的条件下将样品的组分转移通过捕获区域。转移通过移动通过细胞标本的溶液的毛细管作用或通过电泳来实现。转移可以以保持与样品的几何对应关系的方式发生，例如，对应于细胞样品的细胞结构。细胞标本的实例包括组织切片如肿瘤组织切片。细胞定位切片比通常切割得稍微厚一些，即25至50微米，允许检测中等和低水平丰度的分子。

9. 发明申请

WO2004013607A3 METHODS, DEVICES AND KITS FOR MULTIPLEX BLOTTING OF BIOLOGICAL SAMPLES FROM MULTI-WELL PLATES 审中-公开
标题翻译：多孔玻璃生物样品的多重蒸煮方法，装置和工具
公开(公告)号：WO2004013607A3
公开(公告)日：2004-04-08
申请号：PCT/US0324225
申请日：2003-08-01
申请人： 20 20 GENESYSTEMS INC , US GOV HEALTH & HUMAN SERV , KNEZEVIC VLADIMIR , HARTMANN DAN-PAUL , EMMERT-BUCK MICHAEL R
发明人： KNEZEVIC VLADIMIR , HARTMANN DAN-PAUL , EMMERT-BUCK MICHAEL R
IPC分类号： B01J19/00 , B01L3/00 , C12Q1/68 , C40B40/06 , C40B40/10 , G01N33/543 , G01N33/53
CPC分类号： B01L3/50255 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体的示例方法是制备多孔样品保持器的生物分子含量的多个实质性复制品的方法。还提供了用于执行所述方法的设备和套件。

10. 发明申请

WO0210751A2 TRANSFER MICRODISSECTION 审中-公开
标题翻译：转移显微镜
公开(公告)号：WO0210751A2
公开(公告)日：2002-02-07
申请号：PCT/US0108095
申请日：2001-03-14
申请人： US GOV HEALTH & HUMAN SERV , EMMERT BUCK MICHAEL R , ENGLERT CHAD R , BONNER ROBERT F , LIOTTA LANCE A
发明人： EMMERT-BUCK MICHAEL R , ENGLERT CHAD R , BONNER ROBERT F , LIOTTA LANCE A
IPC分类号： G01N1/28 , G01N33/50 , G01N33/543
CPC分类号： G01N33/5005 , G01N1/2813 , G01N33/54386 , G01N2001/284
摘要： The present disclosure concerns methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. In one disclosed example, the method selectively transfers biomolecules from a target region of interest in a biological sample (such as a tissue section). The transfer may occur, for example, by selectively focally altering a characteristic of a transfer layer adjacent the target region, such that the biomolecules can move through the altered area of the transfer layer. In particular examples, the transfer layer is altered by focally increasing a permeability of the transfer layer, for example by removing a focal portion of the transfer layer, and transporting the biomolecules through the altered region of the transfer layer, to microdissect the biomolecules of interest from the biological sample. In yet other embodiments, the microdissected biomolecules can be applied to an analysis substrate containing an identification molecule, such as a nucleic acid array, layered expression scan, or wells containing antibodies. Transfer microdissection allows biomolecules from regions of interest in the biological specimen to be selectively analyzed. For example, nests of highly atypical or metastatic cells in a tumor section can be analyzed for differential expression of certain proteins.
摘要翻译：本公开涉及用于分析生物材料（例如细胞或其它样品）的方法，系统和装置。在一个公开的实施例中，该方法选择性地将生物分子从目标感兴趣区域转移到生物样品（例如组织切片）中。转移可以例如通过选择性地改变邻近目标区域的转移层的特性，使得生物分子可以移动通过转移层的改变的区域而发生。在具体实例中，转移层通过聚焦增加转移层的渗透性而被改变，例如通过去除转移层的焦点部分，以及将生物分子传输通过转移层的改变区域，以显微分解感兴趣的生物分子来自生物样品。在其他实施方案中，显微切割的生物分子可以应用于含有鉴定分子，如核酸阵列，分层表达扫描或含有抗体的孔的分析底物。转移显微切割允许选择性分析来自生物样本中感兴趣区域的生物分子。例如，可以分析肿瘤部分中的非典型或转移性细胞的巢，以鉴别某些蛋白质的差异表达。

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